Original paper
Optimizing genome editing strategy by primer-extension-mediated sequencing
Abstract
Efficient and precise genome editing is essential for clinical applications and generating animal models, which requires engineered nucleases with high editing ability while low off-target activity. Here we present a high-throughput sequencing method, primer-extension-mediated sequencing (PEM-seq), to comprehensively assess both editing ability and specificity of engineered nucleases. We showed CRISPR/Cas9-generated breaks could lead to...
Paper Details
Title
Optimizing genome editing strategy by primer-extension-mediated sequencing
Published Date
Mar 26, 2019
Journal
Volume
5
Issue
1
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Notes
History