Jiazhi Hu
Peking University
Publications 20
#1Yongfeng Yang (PKU: Peking University)H-Index: 2
#2Chuanzhen Yang (PKU: Peking University)H-Index: 2
Last.Xiaofeng Zheng (PKU: Peking University)H-Index: 19
view all 8 authors...
The DNA damage response (DDR) is essential for maintaining genome integrity. Mounting evidence reveals that protein modifications play vital roles in the DDR. Here, we show that USP38 is involved in the DDR by regulating the activity of HDAC1. In response to DNA damage, USP38 interacted with HDAC1 and specifically removed the K63-linked ubiquitin chain promoting the deacetylase activity of HDAC1. As a result, HDAC1 was able to deacetylate H3K56. USP38 deletion resulted in persistent focal accumu...
#1Jianhang Yin (PKU: Peking University)H-Index: 1
#2Mengzhu Liu (PKU: Peking University)H-Index: 1
Last.Jiazhi Hu (PKU: Peking University)H-Index: 12
view all 9 authors...
Efficient and precise genome editing is essential for clinical applications and generating animal models, which requires engineered nucleases with high editing ability while low off-target activity. Here we present a high-throughput sequencing method, primer-extension-mediated sequencing (PEM-seq), to comprehensively assess both editing ability and specificity of engineered nucleases. We showed CRISPR/Cas9-generated breaks could lead to chromosomal translocations and large deletions by PEM-seq. ...
1 CitationsSource
#1Cheng-Sheng LeeH-Index: 2
#2Jiazhi HuH-Index: 12
Last.Frederick W. AltH-Index: 157
view all 3 authors...
RAG endonuclease initiates V(D)J recombination by binding to the recombination signal sequences (RSSs), introducing DNA breaks between two V(D)J gene segments, and mediating their joining. This process is tightly regulated to generate diverse antigen receptor repertoires and prevent aberrant events that could cause genomic translocations/deletions and lymphoid cancer. RAG can track directionally over chromosomal loop domain in which they reside. Tracking is evidenced by cleavage/joining of short...
#1Jianhang YinH-Index: 1
#2Mengzhu LiuH-Index: 1
Last.Jiazhi HuH-Index: 12
view all 4 authors...
#1Jianhang YinH-Index: 1
#2Mengzhu LiuH-Index: 1
Last.Jiazhi HuH-Index: 12
view all 4 authors...
#1Erwei Zuo (CAS: Chinese Academy of Sciences)H-Index: 6
#2Xiaona Huo (CAS: Chinese Academy of Sciences)H-Index: 2
Last.Hui Yang (CAS: Chinese Academy of Sciences)H-Index: 19
view all 18 authors...
The CRISPR/Cas9 system has become an efficient gene editing method for generating cells carrying precise gene mutations, including the rearrangement and deletion of chromosomal segments. However, whether an entire chromosome could be eliminated by this technology is still unknown. Here we demonstrate the use of the CRISPR/Cas9 system to eliminate targeted chromosomes. Using either multiple cleavages induced by a single-guide RNA (sgRNA) that targets multiple chromosome-specific sites or a cockta...
34 CitationsSource
#1Ling Guan (THU: Tsinghua University)H-Index: 1
#2Peng He (THU: Tsinghua University)H-Index: 1
Last.Daochun Kong (THU: Tsinghua University)H-Index: 13
view all 13 authors...
3 CitationsSource
#1Bochao Liu (PKU: Peking University)H-Index: 1
#2Jiazhi Hu (PKU: Peking University)H-Index: 12
Last.Daochun Kong (PKU: Peking University)H-Index: 13
view all 4 authors...
Abstract During DNA replication in eukaryotic cells, short single-stranded DNA segments known as Okazaki fragments are first synthesized on the lagging strand. The Okazaki fragments originate from ∼35-nucleotide-long RNA-DNA primers. After Okazaki fragment synthesis, these primers must be removed to allow fragment joining into a continuous lagging strand. To date, the models of enzymatic machinery that removes the RNA-DNA primers have come almost exclusively from biochemical reconstitution studi...
14 CitationsSource
#1Zhaoqing Ba (HHMI: Howard Hughes Medical Institute)H-Index: 4
#2Jiazhi Hu (HHMI: Howard Hughes Medical Institute)H-Index: 12
Last.Frederick W. Alt (HHMI: Howard Hughes Medical Institute)H-Index: 157
view all 6 authors...
The N-terminal variable regions of immunoglobulin (Ig) heavy (IgH) and light (IgL) chains are involved in specific antigen binding and assembled from germline variable (V), diversity (D), and joining (J) gene segments through a somatic recombination reaction known as V(D)J recombination. V(D)J recombination is initiated by RAG endonuclease and completed by non-homologous DNA end-joining. V(D)J recombination is tightly regulated in the contexts of order, lineage, and allelic exclusion. For IgL th...
#1Lijuan Zhao (HHMI: Howard Hughes Medical Institute)H-Index: 5
#2Richard L. Frock (HHMI: Howard Hughes Medical Institute)H-Index: 14
Last.Frederick W. Alt (HHMI: Howard Hughes Medical Institute)H-Index: 157
view all 7 authors...
T cell antigen receptor δ ( Tcrd ) variable region exons are assembled by RAG-initiated V(D)J recombination events in developing γδ thymocytes. Here, we use linear amplification–mediated high-throughput genome-wide translocation sequencing (LAM-HTGTS) to map hundreds of thousands of RAG-initiated Tcrd D segment ( Trdd1 and Trdd2 ) rearrangements in CD4−CD8− double-negative thymocyte progenitors differentiated in vitro from bone marrow–derived hematopoietic stem cells. We find that Trdd2 joins di...
17 CitationsSource