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Jiazhi Hu
Peking University
Molecular biologyV(D)J recombinationGeneticsDNABiology
18Publications
12H-index
835Citations
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Publications 26
Newest
#1Xiaojing Liu (CAS: Chinese Academy of Sciences)
#2Tingting Liu (CAS: Chinese Academy of Sciences)H-Index: 1
Last. Liu Daisy Liu (CAS: Chinese Academy of Sciences)H-Index: 1
view all 20 authors...
Programmed DNA recombination in mammalian cells occurs predominantly in a directional manner. While random DNA breaks are typically repaired both by deletion and by inversion at approximately equal proportions, V(D)J and class switch recombination (CSR) of immunoglobulin heavy chain gene overwhelmingly delete intervening sequences to yield productive rearrangement. What factors channel chromatin breaks to deletional CSR in lymphocytes is unknown. Integrating CRISPR knockout and chemical perturba...
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#1Yongfeng Yang (PKU: Peking University)H-Index: 2
#2Chuanzhen Yang (PKU: Peking University)H-Index: 2
Last. Xiaofeng Zheng (PKU: Peking University)H-Index: 19
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The DNA damage response (DDR) is essential for maintaining genome integrity. Mounting evidence reveals that protein modifications play vital roles in the DDR. Here, we show that USP38 is involved in the DDR by regulating the activity of HDAC1. In response to DNA damage, USP38 interacted with HDAC1 and specifically removed the K63-linked ubiquitin chain promoting the deacetylase activity of HDAC1. As a result, HDAC1 was able to deacetylate H3K56. USP38 deletion resulted in persistent focal accumu...
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#1Jianhang Yin (PKU: Peking University)H-Index: 1
#2Mengzhu Liu (PKU: Peking University)H-Index: 1
Last. Jiazhi Hu (PKU: Peking University)H-Index: 12
view all 9 authors...
Efficient and precise genome editing is essential for clinical applications and generating animal models, which requires engineered nucleases with high editing ability while low off-target activity. Here we present a high-throughput sequencing method, primer-extension-mediated sequencing (PEM-seq), to comprehensively assess both editing ability and specificity of engineered nucleases. We showed CRISPR/Cas9-generated breaks could lead to chromosomal translocations and large deletions by PEM-seq. ...
1 CitationsSource
#1Zhaoqing Ba (Boston Children's Hospital)
#2Suvi Jain (Boston Children's Hospital)
Last. Frederick W. Alt (Boston Children's Hospital)H-Index: 157
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Immunoglobulin heavy (IgH) and light (IgL) chain variable region exons are assembled from V, D and J segments by V(D)J recombination, which is initiated by RAG1/RAG2 endonuclease (RAG) that introduces DNA double-stranded breaks (DSBs) between a pair of V, D, and J coding segments and flanking recombination signal sequences (RSSs). IgH V(D)J recombination is ordered, with D-JH joining occurring before appendage of a VH to a DJH intermediate in pro-B cells. In pre-B cells, a single-step VL-to-JL r...
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#1Cheng-Sheng LeeH-Index: 2
#2Jiazhi HuH-Index: 12
Last. Frederick W. AltH-Index: 157
view all 3 authors...
RAG endonuclease initiates V(D)J recombination by binding to the recombination signal sequences (RSSs), introducing DNA breaks between two V(D)J gene segments, and mediating their joining. This process is tightly regulated to generate diverse antigen receptor repertoires and prevent aberrant events that could cause genomic translocations/deletions and lymphoid cancer. RAG can track directionally over chromosomal loop domain in which they reside. Tracking is evidenced by cleavage/joining of short...
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#1Jianhang YinH-Index: 1
#2Mengzhu LiuH-Index: 1
Last. Jiazhi HuH-Index: 12
view all 4 authors...
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#1Jianhang YinH-Index: 1
#2Mengzhu LiuH-Index: 1
Last. Jiazhi HuH-Index: 12
view all 4 authors...
Source
#1Erwei Zuo (CAS: Chinese Academy of Sciences)H-Index: 6
#2Xiaona Huo (CAS: Chinese Academy of Sciences)H-Index: 2
Last. Hui Yang (CAS: Chinese Academy of Sciences)H-Index: 19
view all 18 authors...
The CRISPR/Cas9 system has become an efficient gene editing method for generating cells carrying precise gene mutations, including the rearrangement and deletion of chromosomal segments. However, whether an entire chromosome could be eliminated by this technology is still unknown. Here we demonstrate the use of the CRISPR/Cas9 system to eliminate targeted chromosomes. Using either multiple cleavages induced by a single-guide RNA (sgRNA) that targets multiple chromosome-specific sites or a cockta...
34 CitationsSource
#1Ling Guan (THU: Tsinghua University)H-Index: 1
#2Peng He (THU: Tsinghua University)H-Index: 1
Last. Daochun Kong (THU: Tsinghua University)H-Index: 13
view all 13 authors...
3 CitationsSource
#1Bochao Liu (PKU: Peking University)H-Index: 1
#2Jiazhi Hu (PKU: Peking University)H-Index: 12
Last. Daochun Kong (PKU: Peking University)H-Index: 13
view all 4 authors...
Abstract During DNA replication in eukaryotic cells, short single-stranded DNA segments known as Okazaki fragments are first synthesized on the lagging strand. The Okazaki fragments originate from ∼35-nucleotide-long RNA-DNA primers. After Okazaki fragment synthesis, these primers must be removed to allow fragment joining into a continuous lagging strand. To date, the models of enzymatic machinery that removes the RNA-DNA primers have come almost exclusively from biochemical reconstitution studi...
14 CitationsSource
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