Jianhang Yin
Peking University
Publications 4
#1Jianhang Yin (PKU: Peking University)H-Index: 1
#2Mengzhu Liu (PKU: Peking University)H-Index: 1
Last.Jiazhi Hu (PKU: Peking University)H-Index: 12
view all 9 authors...
Efficient and precise genome editing is essential for clinical applications and generating animal models, which requires engineered nucleases with high editing ability while low off-target activity. Here we present a high-throughput sequencing method, primer-extension-mediated sequencing (PEM-seq), to comprehensively assess both editing ability and specificity of engineered nucleases. We showed CRISPR/Cas9-generated breaks could lead to chromosomal translocations and large deletions by PEM-seq. ...
1 CitationsSource
#1Jianhang YinH-Index: 1
#2Mengzhu LiuH-Index: 1
Last.Jiazhi HuH-Index: 12
view all 4 authors...
#1Erwei Zuo (CAS: Chinese Academy of Sciences)H-Index: 6
#2Xiaona Huo (CAS: Chinese Academy of Sciences)H-Index: 2
Last.Hui Yang (CAS: Chinese Academy of Sciences)H-Index: 19
view all 18 authors...
The CRISPR/Cas9 system has become an efficient gene editing method for generating cells carrying precise gene mutations, including the rearrangement and deletion of chromosomal segments. However, whether an entire chromosome could be eliminated by this technology is still unknown. Here we demonstrate the use of the CRISPR/Cas9 system to eliminate targeted chromosomes. Using either multiple cleavages induced by a single-guide RNA (sgRNA) that targets multiple chromosome-specific sites or a cockta...
34 CitationsSource