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Probiotic With or Without Fiber Controls Body Fat Mass, Associated With Serum Zonulin, in Overweight and Obese Adults—Randomized Controlled Trial

Published on Nov 1, 2016in EBioMedicine6.68
· DOI :10.1016/j.ebiom.2016.10.036
Lotta Stenman5
Estimated H-index: 5
(DuPont),
Markus J. Lehtinen8
Estimated H-index: 8
(DuPont)
+ 12 AuthorsSampo J. Lahtinen29
Estimated H-index: 29
(DuPont)
Sources
Abstract
Abstract Background The gut microbiota is interlinked with obesity, but direct evidence of effects of its modulation on body fat mass is still scarce. We investigated the possible effects of Bifidobacterium animalis ssp. lactis 420 (B420) and the dietary fiber Litesse® Ultra polydextrose (LU) on body fat mass and other obesity-related parameters. Methods 225 healthy volunteers (healthy, BMI 28–34.9) were randomized into four groups (1:1:1:1), using a computer-generated sequence, for 6months of double-blind, parallel treatment: 1) Placebo, microcrystalline cellulose, 12g/d; 2) LU, 12g/d; 3) B420, 10 10 CFU/d in microcrystalline cellulose, 12g/d; 4) LU+B420, 12g+10 10 CFU/d. Body composition was monitored with dual-energy X-ray absorptiometry, and the primary outcome was relative change in body fat mass, comparing treatment groups to Placebo. Other outcomes included anthropometric measurements, food intake and blood and fecal biomarkers. The study was registered in Clinicaltrials.gov (NCT01978691). Findings There were marked differences in the results of the Intention-To-Treat (ITT; n =209) and Per Protocol (PP; n =134) study populations. The PP analysis included only those participants who completed the intervention with >80% product compliance and no antibiotic use. In addition, three participants were excluded from DXA analyses for PP due to a long delay between the end of intervention and the last DXA measurement. There were no significant differences between groups in body fat mass in the ITT population. However, LU+B420 and B420 seemed to improve weight management in the PP population. For relative change in body fat mass, LU+B420 showed a−4.5% (−1.4kg, P =0.02, N =37) difference to the Placebo group, whereas LU (+0.3%, P =1.00, N =35) and B420 (−3.0%, P =0.28, N =24) alone had no effect (overall ANOVA P =0.095, Placebo N =35). A post-hoc factorial analysis was significant for B420 (−4.0%, P =0.002 vs. Placebo). Changes in fat mass were most pronounced in the abdominal region, and were reflected by similar changes in waist circumference. B420 and LU+B420 also significantly reduced energy intake compared to Placebo. Changes in blood zonulin levels and hsCRP were associated with corresponding changes in trunk fat mass in the LU+B420 group and in the overall population. There were no differences between groups in the incidence of adverse events. Discussion This clinical trial demonstrates that a probiotic product with or without dietary fiber controls body fat mass. B420 and LU+B420 also reduced waist circumference and food intake, whereas LU alone had no effect on the measured outcomes.
  • References (49)
  • Citations (32)
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References49
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#1Lotta Stenman (DuPont)H-Index: 5
#2Rémy Burcelin (French Institute of Health and Medical Research)H-Index: 62
Last. Sampo J. Lahtinen (DuPont)H-Index: 29
view all 3 authors...
Changes in the gut microbiota are associated with metabolic disorders, such as overweight and elevated blood glucose. Mouse studies have shown that gut microbiota can regulate metabolism with a mechanism related to gut barrier function. An impaired gut barrier permits the translocation of bacteria and their components which, when in contact with the sub-mucosal immune system, evoke metabolic inflammation and distract signalling in metabolically active tissues. Despite thorough research of the to...
29 CitationsSource
#2Nabil E. BoutagyH-Index: 8
Last. Matthew W. HulverH-Index: 34
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Objective The objective was to determine the effects of the probiotic, VSL#3, on body and fat mass, insulin sensitivity, and skeletal muscle substrate oxidation following 4 weeks of a high-fat diet. Methods Twenty non-obese males (18-30 years) participated in the study. Following a 2-week eucaloric control diet, participants underwent dual X-ray absorptiometry to determine body composition, an intravenous glucose tolerance test to determine insulin sensitivity, and a skeletal muscle biopsy for m...
19 CitationsSource
#1Lotta Stenman (DuPont)H-Index: 5
#2Aurélie Waget (French Institute of Health and Medical Research)H-Index: 20
Last. Sampo J. Lahtinen (DuPont)H-Index: 29
view all 7 authors...
Background Gut microbiota is now known to control glucose metabolism. Previous studies have shown that probiotics and prebiotics may improve glucose metabolism, but their effects have not been studied in combination with drug therapy. The aim of this study was to investigate whether probiotics and prebiotics combined with drug therapy affect diabetic outcomes.
19 CitationsSource
#1Xavier Pi-SunyerH-Index: 20
#2A. AstrupH-Index: 96
Last. John WildingH-Index: 58
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BACKGROUND Obesity is a chronic disease with serious health consequences, but weight loss is difficult to maintain through lifestyle intervention alone. Liraglutide, a glucagonlike peptide-1 analogue, has been shown to have potential benefit for weight management at a once-daily dose of 3.0 mg, injected subcutaneously. METHODS We conducted a 56-week, double-blind trial involving 3731 patients who did not have type 2 diabetes and who had a body-mass index (BMI; the weight in kilograms divided by ...
467 CitationsSource
#1Lucile Garidou (Paul Sabatier University)H-Index: 14
#2Céline Pomié (Paul Sabatier University)H-Index: 10
Last. Rémy Burcelin (Paul Sabatier University)H-Index: 62
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Summary A high-fat diet (HFD) induces metabolic disease and low-grade metabolic inflammation in response to changes in the intestinal microbiota through as-yet-unknown mechanisms. Here, we show that a HFD-derived ileum microbiota is responsible for a decrease in Th17 cells of the lamina propria in axenic colonized mice. The HFD also changed the expression profiles of intestinal antigen-presenting cells and their ability to generate Th17 cells in vitro. Consistent with these data, the metabolic p...
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#1Jun-ichi MinamiH-Index: 5
#2Shizuki KondoH-Index: 13
Last. Taeko ShimodaH-Index: 2
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#1K. A. Elisa Kallio (UH: University of Helsinki)H-Index: 4
#2Katja A. Hätönen (National Institute for Health and Welfare)H-Index: 8
Last. Pirkko J. Pussinen (UH: University of Helsinki)H-Index: 42
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Aims Circulating lipopolysaccharides (LPSs), associated with both infection and inflammation, may arise from the gastrointestinal tract microbiota, and the levels may be affected by daily nutrition. We investigated whether nutrient intake affects the association of serum LPS activity with prevalent obesity, metabolic syndrome (MetS), diabetes, and coronary heart disease (CHD) and with the risk of incident CHD events.
43 CitationsSource
#1Alvin Ibarra (DuPont)H-Index: 15
#2Nerys M. Astbury (Mount Sinai St. Luke's and Mount Sinai Roosevelt)H-Index: 13
Last. Kirsti Tiihonen (DuPont)H-Index: 21
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Abstract Introduction: Dietary fibers help to control energy intake and reduce the risk of developing obesity. Recent studies show that the consumption of polydextrose reduces energy intake at a subsequent meal. In this systematic review and meta-analysis we examine the subsequent effects of polydextrose on different levels of energy intake (EI). Method: The review followed the PRISMA methodology. Meta-analyses were expressed as Standardized Mean Difference (SMD). A linear regression approach wa...
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#1Lucia PacificoH-Index: 32
#2Enea BonciH-Index: 22
Last. Claudio ChiesaH-Index: 32
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AIM: To investigate the potential association of circulating zonulin with the stage of liver disease in obese children with biopsy-confirmed nonalcoholic fatty liver disease (NAFLD). METHODS: A case-control study was performed. Cases were 40 obese children with NAFLD. The diagnosis of NAFLD was based on magnetic resonance imaging (MRI) with high hepatic fat fraction (HFF ≥ 5%), and confirmed by liver biopsy with ≥ 5% of hepatocytes containing macrovesicular fat. Controls were selected from obese...
19 CitationsSource
#1Lotta Stenman (DuPont)H-Index: 5
#2Aurélie Waget (French Institute of Health and Medical Research)H-Index: 20
Last. Sampo J. Lahtinen (DuPont)H-Index: 29
view all 6 authors...
Alterations of the gut microbiota and mucosal barrier are linked with metabolic diseases. Our aim was to investigate the potential benefit of the potential probiotic Bifidobacterium animalis ssp. lactis 420 in reducing high-fat diet-induced body weight gain and diabetes in mice. In the obesity model, C57Bl/6J mice were fed a high-fat diet (60 energy %) for 12 weeks, and gavaged daily with B. lactis 420 (109 cfu) or vehicle. In the diabetes model, mice were fed a high-fat, ketogenic diet (72 ener...
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The growing worldwide epidemic of obesity and associated metabolic health comorbidities has resulted in an urgent need for safe and efficient nutritional solutions. The research linking obesity with gut microbiota dysbiosis has led to a hypothesis that certain bacterial strains could serve as probiotics helping in weight management and metabolic health. In the search for such strains, the effect of Bifidobacterium animalis subsp. lactis 420 (B420) on gut microbiota and metabolic health, and the ...
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#1Tatiane Ferreira da Silva (UNESP: Sao Paulo State University)H-Index: 3
Last. Ana Lúcia Barretto Penna (UNESP: Sao Paulo State University)H-Index: 14
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AbstractObesity is currently considered a global epidemic and it leads to several alterations on the human body and its metabolism. There are evidences showing that the intestinal microbiota can in...
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#2Jack AaH-Index: 1
Last. Jak McDonald (Imperial College London)
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In an exploratory, block-randomised, parallel, double-blind, single-centre, placebo-controlled superiority study (ISRCTN12562026, funded by Cultech Ltd), 220 Bulgarian participants (30 to 65 years old) with BMI 25–34.9 kg/m2 received Lab4P probiotic (50 billion/day) or a matched placebo for 6 months. Participants maintained their normal diet and lifestyle. Primary outcomes were changes in body weight, BMI, waist circumference (WC), waist-to-height ratio (WtHR), blood pressure and plasma lipids. ...
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#2Sepideh Soltani (IUMS: Iran University of Medical Sciences)H-Index: 4
Last. Seyed Mohammad Mazloomi (Shiraz University of Medical Sciences)H-Index: 7
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#1Alessio Fasano (Harvard University)H-Index: 85
Improved hygiene leading to reduced exposure to microorganisms has been implicated as one possible cause for the recent “epidemic” of chronic inflammatory diseases (CIDs) in industrialized countries. That is the essence of the hygiene hypothesis that argues that rising incidence of CIDs may be, at least in part, the result of lifestyle and environmental changes that have made us too “clean” for our own good, so causing changes in our microbiota. Apart from genetic makeup and exposure to environm...
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Translocation of microbiome-derived lipopolysaccharide (LPS) to the bloodstream (metabolic endotoxaemia) is associated with a significantly increased risk of cardiovascular diseases (CVD); however, the direction of this association is not fully understood. It has been revealed by some studies that alterations in the intestinal microbiota (dysbiosis) lead to increased intestinal permeability and translocation of LPS to the blood circulation. LPS may trigger toll-like receptor 4- (TLR-4) mediated ...
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Abstract Several studies are contributing to the better understanding of the impact of probiotics and prebiotics on the modulation of the intestinal microbiota and subsequent effects on the host’s health. This review aimed to discuss the results of studies using different experimental models to evaluate the impact of the supplementation with probiotics and/or prebiotics on the different risk factors related to metabolic syndrome (MetS). A better understanding of the daily supplementation of prob...
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