Probiotic With or Without Fiber Controls Body Fat Mass, Associated With Serum Zonulin, in Overweight and Obese Adults—Randomized Controlled Trial

Published on Nov 1, 2016in EBioMedicine
· DOI :10.1016/j.ebiom.2016.10.036
Lotta Stenman5
Estimated H-index: 5
(DuPont),
Markus J. Lehtinen6
Estimated H-index: 6
(DuPont)
+ 12 AuthorsSampo J. Lahtinen26
Estimated H-index: 26
(DuPont)
Abstract
Abstract Background The gut microbiota is interlinked with obesity, but direct evidence of effects of its modulation on body fat mass is still scarce. We investigated the possible effects of Bifidobacterium animalis ssp. lactis 420 (B420) and the dietary fiber Litesse® Ultra polydextrose (LU) on body fat mass and other obesity-related parameters. Methods 225 healthy volunteers (healthy, BMI 28–34.9) were randomized into four groups (1:1:1:1), using a computer-generated sequence, for 6months of double-blind, parallel treatment: 1) Placebo, microcrystalline cellulose, 12g/d; 2) LU, 12g/d; 3) B420, 10 10 CFU/d in microcrystalline cellulose, 12g/d; 4) LU+B420, 12g+10 10 CFU/d. Body composition was monitored with dual-energy X-ray absorptiometry, and the primary outcome was relative change in body fat mass, comparing treatment groups to Placebo. Other outcomes included anthropometric measurements, food intake and blood and fecal biomarkers. The study was registered in Clinicaltrials.gov (NCT01978691). Findings There were marked differences in the results of the Intention-To-Treat (ITT; n =209) and Per Protocol (PP; n =134) study populations. The PP analysis included only those participants who completed the intervention with >80% product compliance and no antibiotic use. In addition, three participants were excluded from DXA analyses for PP due to a long delay between the end of intervention and the last DXA measurement. There were no significant differences between groups in body fat mass in the ITT population. However, LU+B420 and B420 seemed to improve weight management in the PP population. For relative change in body fat mass, LU+B420 showed a−4.5% (−1.4kg, P =0.02, N =37) difference to the Placebo group, whereas LU (+0.3%, P =1.00, N =35) and B420 (−3.0%, P =0.28, N =24) alone had no effect (overall ANOVA P =0.095, Placebo N =35). A post-hoc factorial analysis was significant for B420 (−4.0%, P =0.002 vs. Placebo). Changes in fat mass were most pronounced in the abdominal region, and were reflected by similar changes in waist circumference. B420 and LU+B420 also significantly reduced energy intake compared to Placebo. Changes in blood zonulin levels and hsCRP were associated with corresponding changes in trunk fat mass in the LU+B420 group and in the overall population. There were no differences between groups in the incidence of adverse events. Discussion This clinical trial demonstrates that a probiotic product with or without dietary fiber controls body fat mass. B420 and LU+B420 also reduced waist circumference and food intake, whereas LU alone had no effect on the measured outcomes.
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References49
Published on Jul 2, 2015in The New England Journal of Medicine 79.26
F. Xavier Pi-Sunyer74
Estimated H-index: 74
,
A.V. Astrup91
Estimated H-index: 91
+ 8 AuthorsChristine B. Jensen22
Estimated H-index: 22
BACKGROUND Obesity is a chronic disease with serious health consequences, but weight loss is difficult to maintain through lifestyle intervention alone. Liraglutide, a glucagonlike peptide-1 analogue, has been shown to have potential benefit for weight management at a once-daily dose of 3.0 mg, injected subcutaneously. METHODS We conducted a 56-week, double-blind trial involving 3731 patients who did not have type 2 diabetes and who had a body-mass index (BMI; the weight in kilograms divided by ...
403 Citations Source Cite
Published on Jan 1, 2015in Journal of Nutritional Science
Junichi Minami3
Estimated H-index: 3
,
Shizuki Kondo10
Estimated H-index: 10
+ 7 AuthorsTaeko Shimoda2
Estimated H-index: 2
Accumulating evidence suggests an association between gut microbiota and the development of obesity, raising the possibility of probiotic administration as a therapeutic approach. Bifidobacterium breve B-3 was found to exhibit an anti-obesity effect on high-fat diet-induced obesity mice. In the present study, a randomised, double-blind, placebo-controlled trial was conducted to evaluate the effect of the consumption of B. breve B-3 on body compositions and blood parameters in adults with a tende...
29 Citations Source Cite
Published on Jul 1, 2015in Cell Metabolism 20.57
Lucile Garidou13
Estimated H-index: 13
(Paul Sabatier University),
Céline Pomié8
Estimated H-index: 8
(Paul Sabatier University)
+ 20 AuthorsSampo J. Lahtinen26
Estimated H-index: 26
Summary A high-fat diet (HFD) induces metabolic disease and low-grade metabolic inflammation in response to changes in the intestinal microbiota through as-yet-unknown mechanisms. Here, we show that a HFD-derived ileum microbiota is responsible for a decrease in Th17 cells of the lamina propria in axenic colonized mice. The HFD also changed the expression profiles of intestinal antigen-presenting cells and their ability to generate Th17 cells in vitro. Consistent with these data, the metabolic p...
79 Citations Source Cite
David J. Brillon12
Estimated H-index: 12
(Cornell University),
B. Zheng1
Estimated H-index: 1
(Cornell University)
+ 1 AuthorsDwight E. Matthews59
Estimated H-index: 59
(Cornell University)
Hydrocortisone was infused overnight into nine normal healthy adults on three occasions at 0, 80, and 200 micrograms.kg-1.h-1, producing plasma cortisol concentrations of 10.6 +/- 1.2, 34.0 +/- 2.0, and 64.9 +/- 4.3 micrograms/dl, respectively. L-[1-13C]leucine, L-[phenyl-2H5]phenylalanine, and L-[2-15N]glutamine were infused during the last 7 h of hypercortisolemia to measure amino acid kinetics. During the last 3.5 h, somatostatin, glucagon, and insulin were infused to reduce the cortisol-indu...
135 Citations Source Cite
Published on Dec 1, 2015in Diabetology & Metabolic Syndrome 2.41
Lotta Stenman5
Estimated H-index: 5
(DuPont),
Aurélie Waget25
Estimated H-index: 25
(French Institute of Health and Medical Research)
+ 4 AuthorsSampo J. Lahtinen26
Estimated H-index: 26
(DuPont)
Background Gut microbiota is now known to control glucose metabolism. Previous studies have shown that probiotics and prebiotics may improve glucose metabolism, but their effects have not been studied in combination with drug therapy. The aim of this study was to investigate whether probiotics and prebiotics combined with drug therapy affect diabetic outcomes.
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Published on Jun 1, 1995in Journal of Nutrition 4.40
Glenn R. Gibson99
Estimated H-index: 99
,
Marcel Roberfroid56
Estimated H-index: 56
(Université catholique de Louvain)
Because the human gut microbiota can play a major role in host health, there is currently some interest in the manipulation of the composition of the gut flora towards a potentially more remedial community. Attempts have been made to increase bacterial groups such as Bifidobacterium and Lactobacillus that are perceived as exerting health-promoting properties. Probiotics, defined as microbial food supplements that beneficially affect the host by improving its intestinal microbial balance, have be...
4,962 Citations Source Cite
Published on Apr 1, 2015in Appetite 3.17
Alvin Ibarra5
Estimated H-index: 5
(DuPont),
Nerys M. Astbury12
Estimated H-index: 12
(Mount Sinai St. Luke's and Mount Sinai Roosevelt)
+ 2 AuthorsKirsti Tiihonen17
Estimated H-index: 17
(DuPont)
Abstract Introduction: Dietary fibers help to control energy intake and reduce the risk of developing obesity. Recent studies show that the consumption of polydextrose reduces energy intake at a subsequent meal. In this systematic review and meta-analysis we examine the subsequent effects of polydextrose on different levels of energy intake (EI). Method: The review followed the PRISMA methodology. Meta-analyses were expressed as Standardized Mean Difference (SMD). A linear regression approach wa...
13 Citations Source Cite
Published on Feb 11, 1998in JAMA 47.66
Urho M. Kujala60
Estimated H-index: 60
,
Jaakko Kaprio128
Estimated H-index: 128
+ 1 AuthorsMarkku Koskenvuo89
Estimated H-index: 89
Context.—Physical activity and fitness are believed to reduce premature mortality, but whether genetic factors modify this effect is not known.Objective.—To investigate leisure physical activity and mortality with respect to familial aggregation of health habits during childhood and factors that may enable some individuals to achieve higher levels of fitness.Design.—Prospective twin cohort study.Setting.—Finland.Subjects.—In 1975, at baseline, 7925 healthy men and 7977 healthy women of the Finni...
424 Citations Source Cite
Published on Nov 1, 2002in Gastroenterology 20.77
Rahzi El Asmar1
Estimated H-index: 1
(University of Maryland, Baltimore),
Pinaki Panigrahi23
Estimated H-index: 23
(University of Maryland, Baltimore)
+ 5 AuthorsAlessio Fasano82
Estimated H-index: 82
(University of Maryland, Baltimore)
Background & Aims: Enteric infections have been implicated in the pathogenesis of both food intolerance and autoimmune diseases secondaryto the impairment of the intestinal barrier. On the basis of our recent discoveryof zonulin, a modulator of small-intestinal tight junctions, we asked whether microorganisms might induce zonulin secretion and increased small-intestinal permeability. Methods: Both ex vivo mammalian small intestines and intestinal cell monolayers were exposed to either pathogenic...
146 Citations Source Cite
Published on Jul 1, 2012in Obesity 4.04
Patrick M. O'Neil26
Estimated H-index: 26
(Medical University of South Carolina),
Steven R. Smith70
Estimated H-index: 70
(Sanford-Burnham Institute for Medical Research)
+ 6 AuthorsWilliam R. Shanahan16
Estimated H-index: 16
(Arena Pharmaceuticals, Inc.)
The BLOOM-DM (Behavioral Modification and Lorcaserin for Obesity and Overweight Management in Diabetes Mellitus) study evaluated efficacy and safety of lorcaserin for weight loss in patients with type 2 diabetes. Secondary objectives included evaluations of glycemic control, lipids, blood pressure, and quality of life. This 1-year, randomized, placebo-controlled trial enrolled 604 patients 1:1:1 to placebo, lorcaserin 10 mg once daily (QD) or lorcaserin 10 mg twice daily (BID). Patients were tre...
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Published on Mar 4, 2017in Gut microbes
Sharon M. Donovan41
Estimated H-index: 41
(University of Illinois at Urbana–Champaign)
ABSTRACTOver the past decade, application of culture-independent, next generation DNA sequencing has dramatically enhanced our understanding of the composition of the gut microbiome and its association with human states of health and disease. Host genetics, age, and environmental factors such as where and who you live with, use of pre-, pro- and antibiotics, exercise and diet influence the short- and long-term composition of the microbiome. Dietary intake is a key determinant of microbiome compo...
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Published on Dec 1, 2017in Scientific Reports 4.12
Ghulam Shere Raza2
Estimated H-index: 2
(University of Oulu),
Heli Putaala7
Estimated H-index: 7
(DuPont)
+ 4 AuthorsKarl-Heinz Herzig44
Estimated H-index: 44
(University of Oulu)
Obesity and dyslipidemia are hallmarks of metabolic and cardiovascular diseases. Polydextrose (PDX), a soluble fiber has lipid lowering effects. We hypothesize that PDX reduces triglycerides and cholesterol by influencing gut microbiota, which in turn modulate intestinal gene expression. C57BL/6 male mice were fed a Western diet (WD) ±75 mg PDX twice daily by oral gavage for 14 days. Body weight and food intake were monitored daily. Fasting plasma lipids, caecal microbiota and gene expression in...
7 Citations Source Cite
Published on Nov 1, 2017in Journal of Functional Foods 3.47
Antoni Caimari16
Estimated H-index: 16
,
Josep Maria del Bas9
Estimated H-index: 9
+ 7 AuthorsLluís Arola32
Estimated H-index: 32
Abstract To evaluate the ability of the heat-killed probiotic Bifidobacterium animalis subsp. lactis CECT 8145 (Ba8145) to ameliorate Metabolic Syndrome (MetS), four groups of male Wistar rats were fed either standard chow (ST) or the obesogenic cafeteria diet (CAF) and were orally supplemented with either heat-killed Ba8145 (10 10 CFU/day) (ST-Ba8145 and CAF-Ba8145 groups) or vehicle (ST-veh and CAF-veh groups) for 84 days. Both ST-Ba8145 and CAF-Ba8145 rats displayed increased energy expenditu...
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Published on Jan 1, 2017in Mediators of Inflammation 3.55
Carmela Colica3
Estimated H-index: 3
,
Ennio Avolio1
Estimated H-index: 1
+ 5 AuthorsLaura Di Renzo18
Estimated H-index: 18
Background. Gut microbiota is implied in obesity, because of its ability to harvest energy from diet, and in the regulation of behavior. Given the link between gut microbiota, body composition, obesity, and anxiety, the aim of this study was to evaluate the effects of a new psychobiotic formulation. Methods. Eligible patients were randomly divided into three groups: psychobiotics oral suspension group (POSG); dietary treatment group (DTG); combined treatment group (CTG). All subjects underwent b...
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Published on Feb 27, 2018in Beneficial Microbes 2.31
Kati Mokkala5
Estimated H-index: 5
(University of Turku),
Pirkko J. Pussinen41
Estimated H-index: 41
(University of Helsinki)
+ 3 AuthorsKirsi Laitinen26
Estimated H-index: 26
(University of Turku)
A disruption in intestinal barrier integrity may predispose individuals to metabolic aberrations, particularly during the vulnerable period of pregnancy. We investigated whether intestinal permeability, as measured by serum zonulin concentration, changes over the duration of pregnancy and whether this change is reflected in lipopolysaccharide (LPS) activity. Second, we tested in a randomised double-blind placebo controlled clinical trial the impact of consuming dietary probiotics and/or long cha...
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Published on Mar 16, 2018in Genes 3.19
George Kunnackal John6
Estimated H-index: 6
(Johns Hopkins University),
Lin Wang2
Estimated H-index: 2
(Johns Hopkins University)
+ 3 AuthorsGerard E. Mullin25
Estimated H-index: 25
Dietary alteration of the gut microbiome is an important target in the treatment of obesity. Animal and human studies have shown bidirectional weight modulation based on the probiotic formulation used. In this study, we systematically reviewed the literature and performed a meta-analysis to assess the impact of prebiotics, probiotics and synbiotics on body weight, body mass index (BMI) and fat mass in adult human subjects. We searched Medline (PubMed), Embase, the Cochrane Library and the Web of...
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Published on Apr 1, 2018in Obesity Reviews 8.48
R. J. Mulders1
Estimated H-index: 1
(Utrecht University),
K. C. G. de Git3
Estimated H-index: 3
(Utrecht University)
+ 3 AuthorsRoger A.H. Adan45
Estimated H-index: 45
(Utrecht University)
Western diets, with high consumption of simple sugars and saturated fats, contribute to the rise in the prevalence of obesity. It now seems clear that high-fat diets cause obesity, at least in part, by modifying the composition and function of the microorganisms that colonize in the gastrointestinal tract, the microbiota. The exact pathways by which intestinal microbiota contribute to obesity remain largely unknown. High-fat diet-induced alterations in intestinal microbiota have been suggested t...
12 Citations Source Cite
Published on Feb 1, 2019in European Journal of Nutrition 4.42
Sebastián Torres10
Estimated H-index: 10
(National Scientific and Technical Research Council),
Emanuel Fabersani3
Estimated H-index: 3
+ 1 AuthorsPaola Gauffin-Cano3
Estimated H-index: 3
(National Scientific and Technical Research Council)
Purpose The first part of this review focuses on the role of cells and molecules of adipose tissue involved in metabolic syndrome-induced inflammation and in the maintenance of this pathology. In the second part of the review, the potential role of probiotics-modulating metabolic syndrome-related inflammatory components is summarized and discussed.
3 Citations Source Cite