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Enhancement of Androgen-Dependent Transcription and Cell Proliferation by Tributyltin and Triphenyltin in Human Prostate Cancer Cells

Published on Dec 1, 2000in Toxicology and Applied Pharmacology 3.58
· DOI :10.1006/taap.2000.9067
Yukako Yamabe4
Estimated H-index: 4
(Kitasato University),
Azusa Hoshino1
Estimated H-index: 1
(Kitasato University)
+ 2 AuthorsSeiichiro Himeno16
Estimated H-index: 16
(Kitasato University)
Cite
Abstract
Tributyltin (TBT) and triphenyltin (TPT) are known to cause imposex, the superimposing of male genitals on female ones, in some species of gastropods. However, the molecular mechanism of the trialkyltin-induced endocrine dysfunction remains to be elucidated. To clarify the effects of organotin compounds on the activation of androgen receptor (AR)-mediated responses in mammals, a LA16 clone that stably expresses androgen-responsive luciferase reporter gene and proliferates in response to androgen was established from human prostate cancer cell line LNCaP. Stimulation of LA16 cells with 100 nM TBT or 1 nM TPT enhanced both AR-dependent transcription of luciferase gene and cell growth to the same extent as those by 1 nM dihydrotestosterone (DHT). TBT or TPT also enhanced the DNA synthesis and expression of endogenous AR target genes such as prostate specific antigen, but not the expression of AR itself. However, an androgen antagonist, flutamide, did not inhibit the TBT- or TPT-induced AR activation. On the other hand, simultaneous treatment of LA16 cells with DHT and TBT or TPT caused highly enhanced effects on AR activation. These results indicate that trialkyltin compounds have an ability to activate AR-mediated transcription in mammalian cells and suggest that a novel target site other than the ligand-binding site of AR is involved in this activation.
  • References (45)
  • Citations (70)
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References45
Newest
Published on May 1, 2000in Toxicological Sciences 3.56
S. Miller1
Estimated H-index: 1
,
D. Kennedy1
Estimated H-index: 1
+ 5 AuthorsD. Busbee1
Estimated H-index: 1
A reporter gene sequence was constructed within a eukaryotic expression vector. The altered plasmid contained 2 sequential estrogen response elements (ERE) coupled to a human phosphoglycerate kinase (PGK) promoter inserted upstream from a cDNA sequence encoding enhanced green fluorescent protein (GFP) with a 3*-polyadenylation signal. The plasmid was linearized and transfected into MCF-7 cells, a human breast cancer-derived line that expresses the estrogen receptor (ER). No selectable marker was...
42 Citations Source Cite
Published on Apr 1, 2000in Food and Chemical Toxicology 3.77
C.L.J. Parfett2
Estimated H-index: 2
(Health Canada),
T. Marquardt1
Estimated H-index: 1
(Health Canada),
R. Pilon3
Estimated H-index: 3
(Health Canada)
Abstract Previous studies in our laboratory have shown that chemical treatments may induce increases in proliferin gene family mRNA accumulation in cultured murine embryonic cells. Proliferin inductions are highly correlated with subsequent promotional outcomes during two-stage focus-formation assays in C3H/10T1/2 cell cultures. In work reported here, the strong affiliation between these two responses was further validated after treating cells with di -n- butyltin dichloride which is a polyvinyl...
10 Citations Source Cite
Published on Feb 1, 2000in Molecular and Cellular Endocrinology 3.69
Béatrice Térouanne20
Estimated H-index: 20
(French Institute of Health and Medical Research),
Bouchra Tahiri3
Estimated H-index: 3
(French Institute of Health and Medical Research)
+ 6 AuthorsCharles Sultan44
Estimated H-index: 44
(French Institute of Health and Medical Research)
Abstract We developed a new stable prostatic cell line expressing the human androgen receptor (AR) and the AR-responsive reporter gene to generate a powerful tool for investigating androgen action and for rapid screening of agonists and antagonists. The AR-deficient PC-3 cells were stably transfected with pSG 5 -puro-hAR and pMMTV-neo-Luc. After selection with puromycin and neomycin, one highly inducible clone was isolated and named PALM, for P C-3- A ndrogen receptor- L uciferase- M MTV. The ex...
117 Citations Source Cite
Published on Dec 23, 1999in Environmental Health Perspectives 8.05
Cameron Q. Sheeler4
Estimated H-index: 4
,
Mark W. Dudley2
Estimated H-index: 2
,
Sohaib A. Khan32
Estimated H-index: 32
We used three yeast genetic systems to investigate the estrogen-like activity of octylphenol (OP), bisphenol-A (BPA), o,p'-DDT, and o, p'-DDE to induce human estrogen receptor (hER) dimerization an...
60 Citations Source Cite
Published on Nov 1, 1999in Environmental Research 5.03
Yolanda Morcillo11
Estimated H-index: 11
(CSIC: Spanish National Research Council),
Cinta Porte51
Estimated H-index: 51
(CSIC: Spanish National Research Council)
Testosterone/estradiol tissue levels and cytochrome P450-dependent aromatase activity have been evaluated in the gastropod Bolinus brandaris. Samples were collected from two locations on the Mediterranean coast (St. Carles and Barcelona) according to their different degrees of imposex development and organotin residues. St. Carles was chosen as a relatively clean site, as only 37% of the females were imposex affected compared to 100% of the females from Barcelona. A strong reduction of estradiol...
74 Citations Source Cite
Published on Aug 1, 1999in Life Sciences 3.45
Takahiro Yanagiya7
Estimated H-index: 7
(Kitasato University),
Nobumasaimura39
Estimated H-index: 39
(Kitasato University)
+ 1 AuthorsSeiichiro Himeno16
Estimated H-index: 16
(Kitasato University)
Abstract Metallothionein (MT) is known to play a predominant role in the protection of cells from cadmium (Cd) toxicity. To investigate other factors involved in Cd resistance, we established Cd-resistant cell lines from simian virus 40-transformed MT null fibroblasts. Cd-resistant MT null cells, Cd-rA7 and Cd-rB5, developed approximately 10-fold resistance to Cd compared to parental cells, but showed no cross-resistance to Zn, Cu, Hg, Ni, As, cisplatin or H 2 O 2 . Accumulation of Cd in the res...
28 Citations Source Cite
Published on Mar 1, 1999in Toxicology and Applied Pharmacology 3.58
Anne Marie Vinggaard43
Estimated H-index: 43
,
Eva Cecilie Bonefeld Joergensen1
Estimated H-index: 1
(AU: Aarhus University),
John Christian Larsen7
Estimated H-index: 7
Abstract Reports on increasing incidences in developmental abnormalities of the human male reproductive tract and the recent identifications of environmental chemicals with antiandrogenic activity necessitate the screening of a larger number of compounds in order to get an overview of potential antiandrogenic chemicals present in our environment. Thus, there is a great need for an effective in vitro screening method for (anti)androgenic chemicals. We have developed a rapid, sensitive, and reprod...
123 Citations Source Cite
Published on Jan 1, 1999in Toxicology and Applied Pharmacology 3.58
Jun-ichi Nishikawa22
Estimated H-index: 22
(Osaka University),
Koichi Saito2
Estimated H-index: 2
(Osaka University)
+ 3 AuthorsTsutomunishihara32
Estimated H-index: 32
(Osaka University)
Abstract The endocrine system exerts important functions in a multitude of physiological processes including embryogenesis, differentiation, and homeostasis. Xenobiotics may modify natural endocrine function and so affect human health and wildlife. It is necessary, therefore, to understand the degree to which xenobiotics can disrupt endocrine systems. The key targets of endocrine disruptors are nuclear hormone receptors, which bind to steroid hormones and regulate their gene transcription. We ha...
296 Citations Source Cite
Okabe T1
Estimated H-index: 1
,
Nawata H1
Estimated H-index: 1
3 Citations
Published on Jul 1, 1998in Toxicology and Applied Pharmacology 3.58
Susan C. Maness9
Estimated H-index: 9
(RTP: Research Triangle Park),
Donald P. McDonnell79
Estimated H-index: 79
(Duke University),
Kevin W. Gaido42
Estimated H-index: 42
(RTP: Research Triangle Park)
Abstract Recent reports have raised new concerns that chemicals in our environment may disrupt normal reproduction and development through inhibition of androgen receptor function. This heightened concern has also increased our need for methods that allow us to characterize chemical interaction with the androgen receptor. In this report we describe an androgen receptor assay that utilizes the HepG2 human hepatoma cell line transiently transfected with the human androgen receptor and an androgen-...
155 Citations Source Cite
Cited By70
Newest
Published on Sep 1, 2019in Chemosphere 5.11
Zhibing Yang1
Estimated H-index: 1
,
Junxia Shi + 4 AuthorsZhenghong Zuo21
Estimated H-index: 21
Source Cite
Published on Oct 1, 2018in Reproductive Toxicology 3.20
Wellerson Rodrigo Scarano14
Estimated H-index: 14
(UNESP: Sao Paulo State University),
Cristiane Figueiredo Pinho2
Estimated H-index: 2
(UNESP: Sao Paulo State University)
+ 3 AuthorsSilvana G.P. Campos13
Estimated H-index: 13
(UNESP: Sao Paulo State University)
Abstract Throughout the last decades, increasing exposure to environmental Endocrine Disruptors Chemicals (EDCs) has been associated with the occurrence of male reproductive disorders, such as impairment of prostate development and function, increase of susceptibility to oncogenesis, Epithelial-Mesenchymal Transition and the metastatic invasive potential. Nevertheless, few studies address the mechanisms involved in these alterations, especially those related to cell junctions, which are hormonal...
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Byung Hwa Min1
Estimated H-index: 1
,
Bo-Mi Kim16
Estimated H-index: 16
+ 3 AuthorsJae-Sung Rhee27
Estimated H-index: 27
(UPR-RP: UPRRP College of Natural Sciences)
Abstract Organotin compounds, such as tributyltin (TBT) and triphenyltin (TPT), have been widely used to control marine fouling. Here, we show that organotin stimulation reduces the hormone levels in the plasma of two economically important aquaculture fish. Blood plasma samples were collected from juvenile red seabream and black rockfish exposed to environmentally realistic concentrations of TBT and TPT for 14 days. The levels of two plasma biomarkers, namely the yolk protein precursor vitellog...
3 Citations Source Cite
Published on May 1, 2018in Environmental Science and Pollution Research 2.91
Ting Deng (Chongqing University), Lei Wu1
Estimated H-index: 1
(Chongqing University)
+ 6 AuthorsWeilin Huang1
Estimated H-index: 1
(RU: Rutgers University)
The occurrence and health risks of organotins (OTs) in the waterworks and source water in the Three Gorges Reservoir Region (TGRR), China were assessed in this study. Water samples were collected at four waterworks (A, B, C, and D) in March and July 2012 to analyze butyltins (BTs) and phenyltins (PTs) using a gas chromatography-mass spectrometry (GC-MS) system. Our results showed that both the waterworks and their nearby water sources were polluted by OTs, with PTs being the most dominant specie...
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Published on Feb 1, 2018in Archives of Toxicology 5.74
Ana Cardoso2
Estimated H-index: 2
(UBI: University of Beira Interior),
Marco G. Alves26
Estimated H-index: 26
(University of Porto)
+ 5 AuthorsLuís Rato17
Estimated H-index: 17
(UBI: University of Beira Interior)
Human exposure to environmental contaminants is widespread. Some of these contaminants have the ability to interfere with adipogenesis, being thus considered as obesogens. Recently, obesogens have been singled out as a cause of male infertility. Sertoli cells (SCs) are essential for male fertility and their metabolic performance, especially glucose metabolism, is under a tight endocrine control, being essential for the success of spermatogenesis. Herein, we studied the impact of the model obesog...
2 Citations Source Cite
Published on Jan 1, 2017
Toshihiro Horiguchi21
Estimated H-index: 21
(National Institute for Environmental Studies)
Basic knowledge of endocrinology or reproductive physiology of prosobranch gastropods is reviewed, focusing on vertebrate-type steroids as possible sex hormones in gastropods. Major points of the view for criticism are steroid-producing cells, enzymes to synthesize and/or metabolize steroids, and functional receptors for steroids. Mechanism of induction and promotion of the development of imposex is also reviewed, regarding six hypotheses proposed as the mechanism by which organotins, such as TB...
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Published on Oct 1, 2016in Chemosphere 5.11
Juan Jiang2
Estimated H-index: 2
(Chongqing University),
Jun-Min Gao1
Estimated H-index: 1
(Chongqing University)
+ 7 AuthorsShixuan He8
Estimated H-index: 8
(CAS: Chinese Academy of Sciences)
Abstract Triphenyltin (TPhT) is a kind of organotin compounds which have been used ubiquitously as herbicide, pesticide, and fungicide in agriculture. The present study provides the possibility to detect and monitor TPhT with normal Raman spectroscopy and surface enhanced Raman scattering (SERS) spectroscopy. Firstly, the complete vibrational Raman spectra characterization of TPhT along with the IR spectroscopy were reported for the first time. Then a wide range of pH values were carried out to ...
9 Citations Source Cite
Published on Aug 1, 2015in Food Chemistry 5.40
Tengfei Li6
Estimated H-index: 6
,
Yongxin She10
Estimated H-index: 10
+ 7 AuthorsHua Shao10
Estimated H-index: 10
Abstract A simple method based on high-performance liquid chromatography–tandem mass spectrometry (LC–MS/MS) was developed for the simultaneous determination of organotins—tri-n-butyltin chloride (TBT), triphenyltin chloride (TPT), dibutyltin dichloride (DBT), and diphenyltin dichloride (DPT)—in plastic food packaging. Samples were prepared by ultrasonic extraction with dichloromethane, followed by dissolution in acetonitrile containing 0.1% formic acid, and purification by an MCX column. The ex...
5 Citations Source Cite
Published on Apr 1, 2015in Medical Hypotheses 1.32
Maria C. Mazioti2
Estimated H-index: 2
(Athens State University),
Konstantinos P. Markakis3
Estimated H-index: 3
(Athens State University),
Athanasios E. Raptis8
Estimated H-index: 8
(Athens State University)
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Published on Nov 1, 2014in Inorganica Chimica Acta 2.43
Sartaj Tabassum25
Estimated H-index: 25
(AMU: Aligarh Muslim University),
Shipra Yadav7
Estimated H-index: 7
(AMU: Aligarh Muslim University),
Farukh Arjmand28
Estimated H-index: 28
(AMU: Aligarh Muslim University)
Abstract The design of metal-based chemotherapeutic drugs depends on choice of ligand framework, therefore, metals incorporated in a bioactive ligand scaffold hold promise for the improved pharmacokinetic profile and better efficacy of drugs. In this context, glycosylated-organotin(IV) antitumor agents are attractive and prove the state-of-the-art design for chemotherapy due to organotin-induced cytotoxicity. In recent years, many organotin(IV) complexes with glycosylated appendage have been dev...
6 Citations Source Cite