Chemistry

Biology

Microbiology

Gastroenterology

Helicobacter pylori

In vitro

Biochemistry

Medicine

Antioxidant

Vitamin E

Journal of Molecular Virology and Immunology

In this study, we aimed to evaluate the effects of vitamins C and E on Helicobacter pylori in vitro, in other words in the absence of the in vivo dynamics of the gastric mucosa.The study included 32 patients with H. pylori-positive nonulcer dyspepsia.Tissue samples from antrum and corpus, which were taken during upper gastrointestinal endoscopy, were Gram stained and seeded onto culture plates supplemented with 7% horse blood for H. pylori culture.Plates were incubated in a microaerophilic environment.Isolated H. pylori strains were identified on Gram-stained smears and with urease test.Different concentrations of vitamins C, E and E+C were added into the Columbia Blood Agar plates containing 7% defibrinated horse blood at appropriate temperature in order to produce vitamin added media.Equal amounts of H. pylori colonies (equivalent to 1McFarland turbidity standard unit) were inoculated onto media plates.Media groups were as follows: 1.Control group, 2. Vitamin C added group, 3. Vitamin E added group, 4. Vita...

In Vitro Effects of Vitamins C and E on Helicobacter pylori