Improved HTGTS for CRISPR/Cas9 off-target detection.

Precise genome editing is essential for scientific research and clinical application. At present, linear amplification-mediated high-throughput genome-wide translocation sequencing (LAM-HTGTS) is one of most effective methods to evaluate the off-target activity of CRISPR-Cas9, which is based on chromosomal translocation and employs a bait DNA double-stranded break (DSB) to capture genome-wide prey DNA DSBs. Here, we described an improved HTGTS...