Global transcriptomic response of Listeria monocytogenes during growth on cantaloupe slices

Published on Feb 1, 2019in Food Microbiology 4.09
· DOI :10.1016/j.fm.2018.09.012
Jihun Kang3
Estimated H-index: 3
(Center for Food Safety and Applied Nutrition),
Laurel S. Burall8
Estimated H-index: 8
(Center for Food Safety and Applied Nutrition)
+ 1 AuthorsAtin R. Datta14
Estimated H-index: 14
(Center for Food Safety and Applied Nutrition)
Abstract
Abstract Understanding a pathogen's response to food environments is imperative to develop effective control strategies as well as to elucidate the impact of foods on virulence potential. The purpose of this study was to assess transcriptional response of Listeria monocytogenes after growth in cantaloupe, as well as its impact on survival in synthetic gastric fluid (SGF). The transcriptional profiles of L. monocytogenes grown in cantaloupe or Brain Heart Infusion (BHI) under refrigeration were compared by a custom-designed microarray. A total of 286 and 175 genes were significantly up- and down-regulated, respectively, in L. monocytogenes grown in cantaloupe as compared to BHI (fold change ≥ 2.5 and adj. P  L. monocytogenes . Enhanced survival of L. monocytogenes in SGF following growth in cantaloupe further demonstrated the impact of cantaloupe-associated growth on the pathogen's subsequent response to a host relevant stress.
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References64
Published on Jun 1, 2004in Journal of Food Protection 1.51
Laura D. Wonderling6
Estimated H-index: 6
,
Darrell O. Bayles4
Estimated H-index: 4
Listeria monocytogenes strain H7762, a frankfurter isolate, was tested to determine whether it was able to survive at 4°C in frankfurter pack fluid (exudate) and to determine whether food exposure affects its acid sensitivity. Cultures were sampled and tested for acid sensitivity by challenge with simulated gastric fluid (SGF). SGF challenges performed immediately after inoculation revealed that between 20 and 26% of the cells survived the full 30 min of SGF challenge regardless of whether the c...
8 Citations Source Cite
Published on Nov 1, 2007in Journal of Food Protection 1.51
Luke D. Peterson3
Estimated H-index: 3
,
Nancy G. Faith18
Estimated H-index: 18
,
Charles J. Czuprynski33
Estimated H-index: 33
Ready-to-eat (RTE) deli meats have been categorized as high-risk foods for contraction of foodborne listeriosis. Several recent listeriosis outbreaks have been associated with the consumption of RTE deli turkey meat. In this study, we examined whether the growth of Listeria monocytogenes F2365 on commercially prepared RTE deli turkey meat causes listerial cells to become more resistant to inactivation by synthetic gastric fluid (SGF). Listerial cells grown on turkey meat to late logarithmic-earl...
20 Citations Source Cite
Published on Jul 1, 2015in Journal of Food Protection 1.51
Dike O. Ukuku12
Estimated H-index: 12
,
Lihan Huang21
Estimated H-index: 21
,
Christopher H. Sommers24
Estimated H-index: 24
For health reasons, people are consuming fresh-cut fruits with or without minimal processing and, thereby, exposing themselves to the risk of foodborne illness if such fruits are contaminated with bacterial pathogens. This study investigated survival and growth parameters of Escherichia coli O157:H7, Salmonella, Listeria monocytogenes, and aerobic mesophilic bacteria transferred from cantaloupe rind surfaces to fresh-cut pieces during fresh-cut preparation. All human bacterial pathogens inoculat...
13 Citations Source Cite
Published on May 1, 2012in Journal of Food Safety 1.27
Kalliopi Rantsiou37
Estimated H-index: 37
(University of Turin),
Marios Mataragas21
Estimated H-index: 21
(Agricultural University of Athens)
+ 1 AuthorsLuca Simone Cocolin47
Estimated H-index: 47
(University of Turin)
ABSTRACT In the present study, the development of reverse transcription-quantitative polymerase chain reaction protocols for three virulence genes (plcA, iap and hly) and the sigB stress response gene that may be applied for the determination of the virulence gene expression of Listeria monocytogenes in situ was reported. When applied to L. monocytogenes strains of different origin (#3, isolated from meat; National Collection of Type Cultures 10527, a collection strain; and #162, isolated from c...
19 Citations Source Cite
Published on Jul 1, 1993in Journal of Bacteriology 3.22
Jean-Jacques Godon35
Estimated H-index: 35
(Institut national de la recherche agronomique),
Christine Delorme18
Estimated H-index: 18
(Institut national de la recherche agronomique)
+ 3 AuthorsPierre Renault41
Estimated H-index: 41
(Institut national de la recherche agronomique)
The Lactococcus lactis subsp. lactis strains isolated from dairy products are auxotrophs for branched-chain amino acids (leucine, isoleucine, and valine), while most strains isolated from nondairy media are prototrophs. We have cloned and sequenced the leu genes from one auxotroph, IL1403. The sequence is 99% homologous to that of the prototroph NCDO2118, which was determined previously. Two nonsense mutations and two small deletions were found in the auxotroph sequence, which might explain the ...
93 Citations Source Cite
Published on Dec 9, 2004in Molecular Microbiology 3.82
Roy D. Sleator36
Estimated H-index: 36
(University College Cork),
Henrike H. Wemekamp-Kamphuis6
Estimated H-index: 6
(Wageningen University and Research Centre)
+ 2 AuthorsColin Hill85
Estimated H-index: 85
(University College Cork)
Summary The ability to colonize the gall bladder has recently been shown to be an important feature of virulent Listeria monocytogenes (J. Hardy, K. P. Francis, M. DeBoer, P. Chu, K. Gibbs, C. H. Contag. Science 303: 851–853, 2004). We suggest that the cytotoxic effects of bile may be increased upon release from the gall bladder into the upper small intestine, and report the identification of a novel bile exclusion system which plays an essential role in intestinal colonization and virulence of ...
110 Citations Source Cite
Published on Dec 1, 2008in International Journal of Food Microbiology 3.45
Sana Mujahid2
Estimated H-index: 2
(Mississippi State University),
Tibor Pechan17
Estimated H-index: 17
(Mississippi State University),
Chinling Wang5
Estimated H-index: 5
(Mississippi State University)
Little is known about whether the growth of L. monocytogenes on a ready-to-eat (RTE) meat matrix has an impact on the bacterium's pathogenic capabilities. In this report, we examined protein expression by L. monocytogenes grown on RTE sliced turkey meat, using L. monocytogenes grown on brain-heart-infusion agar as a control. Total protein fractions of L. monocytogenes from both growth conditions were extracted and compared by two-dimensional gel electrophoresis. Seventy-seven proteins expressed ...
18 Citations Source Cite
Published on Nov 15, 2008in Applied and Environmental Microbiology 3.63
Amy Ream2
Estimated H-index: 2
(United States Department of Agriculture)
To study how Listeria monocytogenes survives and grows in ultrahigh-temperature-processed (UHT) skim milk, microarray technology was used to monitor the gene expression profiles of strain F2365 in UHT skim milk. Total RNA was isolated from strain F2365 in UHT skim milk after 24 h of growth at 4°C, labeled with fluorescent dyes, and hybridized to “custom-made” commercial oligonucleotide (35-mers) microarray chips containing the whole genome of L. monocytogenes strain F2365. Compared to L. monocyt...
52 Citations Source Cite
Published on Mar 21, 2012in PLOS ONE 2.77
Pongpan Laksanalamai6
Estimated H-index: 6
(Center for Food Safety and Applied Nutrition),
Scott A. Jackson18
Estimated H-index: 18
(Center for Food Safety and Applied Nutrition)
+ 1 AuthorsAtin R. Datta14
Estimated H-index: 14
(Center for Food Safety and Applied Nutrition)
Listeria monocytogenes, a foodborne bacterial pathogen, causes invasive and febrile gastroenteritis forms of listeriosis in humans. Both invasive and febrile gastroenteritis listeriosis is caused mostly by serotypes 1/2a, 1/2b and 4b strains. The outbreak strains of serotype 1/2a and 4b could be further classified into several epidemic clones but the genetic bases for the diverse pathophysiology have been unsuccessful. DNA microarray provides an important tool to scan the entire genome for genet...
26 Citations Source Cite
Published on Jan 31, 2011in International Journal of Food Microbiology 3.45
Brigitte Carpentier7
Estimated H-index: 7
(ANSES),
Olivier Cerf6
Estimated H-index: 6
Abstract To understand why Listeria monocytogenes may persist in food industry equipment and premises, notably at low temperature, scientific studies have so far focused on adhesion potential, biofilm forming ability, resistance to desiccation, acid and heat, tolerance to increased sublethal concentration of disinfectants or resistance to lethal concentrations. Evidence from studies in processing plants shows that the factors associated with the presence of L. monocytogenes are those that favor ...
374 Citations Source Cite
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