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Global transcriptomic response of Listeria monocytogenes during growth on cantaloupe slices

Published on Feb 1, 2019in Food Microbiology 4.09
· DOI :10.1016/j.fm.2018.09.012
Jihun Kang3
Estimated H-index: 3
(CFSAN: Center for Food Safety and Applied Nutrition),
Laurel S. Burall10
Estimated H-index: 10
(CFSAN: Center for Food Safety and Applied Nutrition)
+ 1 AuthorsAtin R. Datta16
Estimated H-index: 16
(CFSAN: Center for Food Safety and Applied Nutrition)
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Abstract
Abstract Understanding a pathogen's response to food environments is imperative to develop effective control strategies as well as to elucidate the impact of foods on virulence potential. The purpose of this study was to assess transcriptional response of Listeria monocytogenes after growth in cantaloupe, as well as its impact on survival in synthetic gastric fluid (SGF). The transcriptional profiles of L. monocytogenes grown in cantaloupe or Brain Heart Infusion (BHI) under refrigeration were compared by a custom-designed microarray. A total of 286 and 175 genes were significantly up- and down-regulated, respectively, in L. monocytogenes grown in cantaloupe as compared to BHI (fold change ≥ 2.5 and adj. P  L. monocytogenes . Enhanced survival of L. monocytogenes in SGF following growth in cantaloupe further demonstrated the impact of cantaloupe-associated growth on the pathogen's subsequent response to a host relevant stress.
  • References (64)
  • Citations (1)
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References64
Newest
Published on Sep 1, 2017in International Journal of Food Microbiology 4.01
Dumitru Macarisin10
Estimated H-index: 10
(CFSAN: Center for Food Safety and Applied Nutrition),
Anna Wooten1
Estimated H-index: 1
(CFSAN: Center for Food Safety and Applied Nutrition)
+ 7 AuthorsYi Chen18
Estimated H-index: 18
(CFSAN: Center for Food Safety and Applied Nutrition)
Abstract Recent listeriosis outbreaks and recalls associated with cantaloupes urge for studies to understand the mechanisms of cantaloupe contamination by Listeria monocytogenes . Postharvest practices such as washing and hydrocooling were suggested to facilitate the contamination of fresh fruits by human pathogens. This study assessed the potential of L. monocytogenes internalization into cantaloupes during dump tank washing and immersion-type hydrocooling in water contaminated with L. monocyto...
8 Citations Source Cite
Published on Aug 1, 2016in International Journal of Food Microbiology 4.01
Dike O. Ukuku22
Estimated H-index: 22
(USDA: United States Department of Agriculture),
David J. Geveke19
Estimated H-index: 19
(USDA: United States Department of Agriculture)
+ 1 AuthorsBrendan A. Niemira26
Estimated H-index: 26
(USDA: United States Department of Agriculture)
Fresh-cut cantaloupes have been associated with outbreaks of Salmonellosis. Minimally processed fresh-cut fruits have a limited shelf life because of deterioration caused by spoilage microflora and physiological processes. The objectives of this study were to use a wet steam process to 1) reduce indigenous spoilage microflora and inoculated populations of Salmonella, Escherichia coli O157:H7 and Listeria monocytogenes on the surface of cantaloupes, and 2) reduce the populations counts in cantalo...
8 Citations Source Cite
Published on Jun 1, 2016in Applied and Environmental Microbiology 4.08
Isha R. Patel12
Estimated H-index: 12
(CFSAN: Center for Food Safety and Applied Nutrition),
Jayanthi Gangiredla8
Estimated H-index: 8
(CFSAN: Center for Food Safety and Applied Nutrition)
+ 4 AuthorsChristopher A. Elkins13
Estimated H-index: 13
(CFSAN: Center for Food Safety and Applied Nutrition)
ABSTRACT Most Escherichia coli strains are nonpathogenic. However, for clinical diagnosis and food safety analysis, current identification methods for pathogenic E. coli either are time-consuming and/or provide limited information. Here, we utilized a custom DNA microarray with informative genetic features extracted from 368 sequence sets for rapid and high-throughput pathogen identification. The FDA Escherichia coli Identification (FDA-ECID) platform contains three sets of molecularly informati...
15 Citations Source Cite
Published on May 1, 2016in Journal of Food Protection 1.56
Adrienne E. H. Shearer11
Estimated H-index: 11
,
Kyle LeStrange2
Estimated H-index: 2
+ 1 AuthorsKalmia E. Kniel20
Estimated H-index: 20
ABSTRACT Whole and cut cantaloupes have been implicated as vehicles in foodborne illness outbreaks of norovirus, salmonellosis, and listeriosis. Preparation methods that minimize pathogen transfer from external surfaces to the edible tissue are needed. Two preparation methods were compared for the transfer of Listeria monocytogenes, Salmonella enterica serovar Typhimurium LT2, murine norovirus, and Tulane virus from inoculated cantaloupe rinds to edible tissue and preparation surfaces. For the f...
2 Citations Source Cite
Published on May 1, 2016in Journal of Food Protection 1.56
Mira Rakic Martinez2
Estimated H-index: 2
,
Jason W. Osborne33
Estimated H-index: 33
+ 2 AuthorsSophia Kathariou28
Estimated H-index: 28
ABSTRACT The 2011 listeriosis outbreak attributed to whole cantaloupe involved several genetically distinct strains of serotypes 1/2a and 1/2b that had not been previously reported in invasive listeriosis outbreaks. Here we investigated the potential of strains from the 2011 cantaloupe outbreak to adhere, survive, and grow on cantaloupe rind and flesh and in juice extracted from cantaloupe at different temperatures (4, 8, and 25°C). All strains were able to adhere and grow, with ~10-fold increas...
12 Citations Source Cite
Published on May 1, 2016in Food Microbiology 4.09
Agni Hadjilouka3
Estimated H-index: 3
(AUA: Agricultural University of Athens),
Christina Molfeta1
Estimated H-index: 1
(AUA: Agricultural University of Athens)
+ 3 AuthorsEleftherios H. Drosinos32
Estimated H-index: 32
(AUA: Agricultural University of Athens)
Abstract The aim of the present study was to assess the expression of key virulence genes, during growth of a Listeria monocytogenes isolate in liquid medium, on melon and rocket at different temperatures and time. For that purpose, BHI broth, rocket and melon were inoculated at 7.0–7.5 log CFU mL −1 or g −1 and stored at 4, 10 and 30 °C. Sampling took place upon inoculation and after 0.5, 6 and 24 h of incubation. The RNA was stabilized and the expression of hly , plcA , plcB , sigB , inlA , in...
12 Citations Source Cite
Published on Feb 1, 2016in Journal of Food Protection 1.56
Danisha Garner1
Estimated H-index: 1
,
Sophia Kathariou28
Estimated H-index: 28
ABSTRACT Foodborne transmission of Listeria monocytogenes was first demonstrated through the investigation of the 1981 Maritime Provinces outbreak involving coleslaw. In the following two decades, most listeriosis outbreaks involved foods of animal origin, e.g., deli meats, hot dogs, and soft cheeses. L. monocytogenes serotype 4b, especially epidemic clones I, II, and Ia, were frequently implicated in these outbreaks. However, since 2008 several outbreaks have been linked to diverse types of fre...
36 Citations Source Cite
Published on Jan 4, 2016in Nucleic Acids Research 11.15
Minoru Kanehisa68
Estimated H-index: 68
(Kyoto University),
Yoko Sato9
Estimated H-index: 9
(Fujitsu)
+ 2 AuthorsMao Tanabe11
Estimated H-index: 11
(Kyoto University)
KEGG (http://www.kegg.jp/ or http://www.genome.jp/kegg/) is an integrated database resource for biological interpretation of genome sequences and other high-throughput data. Molecular functions of genes and proteins are associated with ortholog groups and stored in the KEGG Orthology (KO) database. The KEGG pathway maps, BRITE hierarchies and KEGG modules are developed as networks of KO nodes, representing high-level functions of the cell and the organism. Currently, more than 4000 complete geno...
1,511 Citations Source Cite
Published on Jan 1, 2016in Methods of Molecular Biology
Minoru Kanehisa68
Estimated H-index: 68
(Kyoto University)
In the era of high-throughput biology it is necessary to develop not only elaborate computational methods but also well-curated databases that can be used as reference for data interpretation. KEGG ( http://www.kegg.jp/ ) is such a reference knowledge base with two specific aims. One is to compile knowledge on high-level functions of the cell and the organism in terms of the molecular interaction and reaction networks, which is implemented in KEGG pathway maps, BRITE functional hierarchies, and ...
18 Citations Source Cite
Published on Oct 1, 2015in Applied and Environmental Microbiology 4.08
Silin Tang4
Estimated H-index: 4
(Cornell University),
Renato H. Orsi20
Estimated H-index: 20
(Cornell University)
+ 3 AuthorsTeresa M. Bergholz15
Estimated H-index: 15
(NDSU: North Dakota State University)
ABSTRACT The foodborne pathogen Listeria monocytogenes is able to survive and grow in ready-to-eat foods, in which it is likely to experience a number of environmental stresses due to refrigerated storage and the physicochemical properties of the food. Little is known about the specific molecular mechanisms underlying survival and growth of L. monocytogenes under different complex conditions on/in specific food matrices. Transcriptome sequencing (RNA-seq) was used to understand the transcription...
15 Citations Source Cite
Cited By1
Newest
Published on Nov 1, 2019in Food Research International 3.58
Alexandre Lamas7
Estimated H-index: 7
(University of Santiago de Compostela),
Patricia Regal13
Estimated H-index: 13
(University of Santiago de Compostela)
+ 3 AuthorsA. Cepeda32
Estimated H-index: 32
(University of Santiago de Compostela)
Abstract In recent years, the development of high-throughput transcriptomic tools such as RNA-seq and microarrays have revolutionized biological scientific research. Despite these techniques being widely used in biomedical research, their introduction into the field of food microbiology is still limited. However, transcriptomic research is of great importance to fully understand the resistance mechanisms and metabolic pathways involved in foodborne pathogen survival from farm to fork. Throughout...
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Published on Apr 1, 2019in Fems Microbiology Letters 1.99
Victor Jayeola2
Estimated H-index: 2
(NCSU: North Carolina State University),
Cameron Parsons3
Estimated H-index: 3
(NCSU: North Carolina State University)
+ 1 AuthorsSophia Kathariou28
Estimated H-index: 28
(NCSU: North Carolina State University)
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