Original paper
A new pH-responsive peptide tag for protein purification
Abstract
This paper describes a new pH-responsive peptide tag that adds a protein reversible precipitation and redissolution character. This peptide tag is a part of a cell surface protein B (CspB) derived from Corynebacterium glutamicum. Proinsulin that genetically fused with a peptide of N-terminal 6, 17, 50, or 250 amino acid residues of CspB showed that the reversible precipitation and redissolution depended on the pH. The transition occurred within...
Paper Details
Title
A new pH-responsive peptide tag for protein purification
Published Date
Jun 1, 2018
Volume
146
Pages
91 - 96
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