Match!
Takahiro Nonaka
University of Tsukuba
4Publications
1H-index
7Citations
Publications 3
Newest
#1Takahiro Nonaka (University of Tsukuba)H-Index: 1
#2Noriko Tsurui (Ajinomoto)H-Index: 1
Last.Kentaro Shiraki (University of Tsukuba)H-Index: 28
view all 5 authors...
Abstract Cell surface protein B (CspB) from Corynebacterium glutamicum is used as a pH-responsive peptide tag to enable a simple solid–liquid separation method for isolating a CspB fusion protein. Here we demonstrate the first application of a CspB tag for the purification of Teriparatide, which is a biologic drug that is prescribed for osteoporosis. The Teriparatide was constructed as CspB50TEV-Teriparatide, comprising 50 amino acid residues of CspB, the cleavage site of TEV protease, and Terip...
Source
#1Takahiro Nonaka (University of Tsukuba)H-Index: 1
#2Noriko Tsurui (Ajinomoto)H-Index: 1
Last.Kentaro Shiraki (University of Tsukuba)H-Index: 28
view all 5 authors...
Abstract This paper describes a new pH-responsive peptide tag that adds a protein reversible precipitation and redissolution character. This peptide tag is a part of a cell surface protein B (CspB) derived from Corynebacterium glutamicum. Proinsulin that genetically fused with a peptide of N-terminal 6, 17, 50, or 250 amino acid residues of CspB showed that the reversible precipitation and redissolution depended on the pH. The transition occurred within a physiological and narrow pH range. A Csp...
2 CitationsSource
#1Shogo Oki (University of Tsukuba)H-Index: 2
#2Takahiro Nonaka (University of Tsukuba)H-Index: 1
Last.Kentaro Shiraki (University of Tsukuba)H-Index: 28
view all 3 authors...
Abstract Protein purification using non-chromatographic methods is a simple technique that avoids costly resin. Recently, a cell surface protein B (CspB) tag has been developed for a pH-responsive tag for protein purification by solid-liquid separation. Proteins fused with the CspB tag show reversible insolubilization at acidic pH that can be used in solid-liquid separation for protein purification. However, brown-color impurities from co-precipitation hamper further analysis of the target prote...
1 CitationsSource
1