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Protein Expression and Purification
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#1Yu Liu (SWU: Southwest University)
#2Yanling Li (SWU: Southwest University)
Last.Yanhua Fan (SWU: Southwest University)H-Index: 20
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Abstract Chitosanase (EC 3.2.1.132) is an important chitosan-degrading enzyme involved in industrial applications. In this study, a chitosanase gene (BbCSN-1) from Beauveria bassiana, an insect fungal pathogen, was cloned and expressed in Pichia pastoris. The amount of BbCSN-1 in the fermentation broth of P. pastoris gradually increased after induction with methanol from one to 6 d, reaching 398 μg/ml on the 6th day. The molecular characteristics of BbCSN-1 were measured with colloidal chitosan ...
#1Selisha Ann Sooklal (University of the Witwatersrand)
#2Charles B. de Koning (University of the Witwatersrand)H-Index: 27
Last.Karl Rumbold (University of the Witwatersrand)H-Index: 14
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Abstract The incorporation of fluorine has been shown to improve the biophysical and bioactive properties of several organic compounds. However, sustainable strategies of fluorination are needed. Fluorinases have the unique ability to catalyse a C-F bond, hence, have vast potential to be applied as biocatalysts in the preparation of fine chemicals. But fluorinases are extremely rare in nature with only five representatives isolated thus far. Moreover, the heterologous expression of fluorinases i...
#1Nan WangH-Index: 4
#2Kevin Yueju Wang (Northeastern State University)H-Index: 5
Last.Dehu LiuH-Index: 4
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Abstract The N-glycosylation process that occurs in the Pichia pastoris protein expression system can have a significant effect on the yield of heterologous glycoproteins secreted from the yeast. The basis of the effect of N-glycosylation on yield, however, has not been elucidated. In order to investigate the effect of N-glycosylation on heterologous protein production, site-directed mutation was performed on five potential N-glycosylation sites of the tetanus toxin fragment C (TetC). Unaltered ...
#1Muhammad Umair Naseem (University of Debrecen)
#2Nadeem Ahmed (University of the Punjab)H-Index: 4
Last.Ahmad Usman Zafar (University of the Punjab)H-Index: 11
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Abstract Consensus interferon (cIFN) is a wholly synthetic therapeutic protein which is used to treat hepatitis C/B and certain types of malignancies. It has short serum half-life, therefore, to maintain its therapeutic level in the human body it requires thrice-weekly administration. Various strategies like PEGylation and micro-encapsulation have been developed during the last few years to enhance the pharmacokinetics of small therapeutic peptides. This study executed the human albumin-fusion t...
#1Gianluca Veggiani (UNG: University of Nova Gorica)
#2Barbara Giabbai (Elettra Sincrotrone Trieste)H-Index: 2
Last.Ario de Marco (UNG: University of Nova Gorica)H-Index: 15
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Abstract Recombinant antibodies can be expressed as fusion constructs in combination with tags which simplify their engineering into reliable and homogeneous immunoreagents by allowing site-specific, 1:1 functionalization. Several tags and corresponding reagents for recombinant protein derivatization have been proposed but benchmarking surveys for the evaluation of their effect on the characteristics of recombinant antibodies have not been reported. In this work we evaluated the impact on expres...
#1Masako Kikuchi (TUAT: Tokyo University of Agriculture and Technology)
#2Naotake Konno (Utsunomiya University)H-Index: 10
Last.Naoto Habu (Utsunomiya University)H-Index: 9
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Abstract Cellouronate is a (1,4)-β-D-glucuronan prepared by TEMPO-mediated oxidation from regenerated cellulose. We have previously isolated a cellouronate-degrading bacterial strain, Brevundimonas sp. SH203, that produces a cellouronate lyase (β-1,4-glucuronan lyase, CUL-I). In this study, the gene encoding CUL-I was cloned, and the recombinant enzyme was heterologously expressed in Escherichia coli. The predicted CUL-I protein is composed of 426 amino acid residues and includes a putative 21-a...
#1Yunpeng Shen (UPR-RP: UPRRP College of Natural Sciences)H-Index: 2
#2Masayo Morishita (UPR-RP: UPRRP College of Natural Sciences)H-Index: 4
Last.Eric di Luccio (UPR-RP: UPRRP College of Natural Sciences)H-Index: 13
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Abstract The nuclear receptor-binding SET Domain (NSD) family consists of NSD1, NSD2/MMSET/WHSC1, and NSD3/WHSC1L1 histone methyltransferases that are crucial for chromatin remodeling. NSDs are implicated in developmental disorders such as Wolf-Hirschhorn and Sotos syndromes as well as various cancers, including t(4; 14)(p16; q32) myeloma, an incurable cancer in plasma cells. NSDs have been the target of intensive study to understand their biological functions more fully and inform anti-cancer d...
#1Lei Jing (Tianjin University of Science and Technology)H-Index: 5
#2Juanjuan Liu (Tianjin University of Science and Technology)
Last.Aipo Diao (Tianjin University of Science and Technology)H-Index: 9
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Abstract An affibody is a 58 amino acids peptide derived from the Z domain of staphylococcal protein A and generally applied in areas such as imaging diagnosis, clinical therapeutics and biotechnology research. To screen for an affibody targeting the immune checkpoint PD-L1, a combinatorial affibody library was generated in yeast using degenerate overlap PCR primers and In-fusion technology. Z-j1 and Z-j2 affibodies targeting the Ig-like V domain of PD-L1 were screened and identified from this c...
#1Marina Y. Linova (University of Southern Denmark)
#2Michael W. Risør (AU: Aarhus University)H-Index: 6
Last.Henrik Karring (University of Southern Denmark)H-Index: 15
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Abstract The SUMO fusion system is widely used to facilitate recombinant expression and production of difficult-to-express proteins. After purification of the recombinant fusion protein, removal of the SUMO-tag is accomplished by the yeast cysteine protease, SUMO protease 1 (Ulp1), which specifically recognizes the tertiary fold of the SUMO domain. At present, the expression of the catalytic domain, residues 403–621, is used for obtaining soluble and biologically active Ulp1. However, we have ob...
#1Sedigheh Mohammadzadeh (Sari Agricultural Sciences and Natural Resources University)
#2Fatemeh Moradian (Sari Agricultural Sciences and Natural Resources University)H-Index: 4
Last.Sylvain Milla (University of Lorraine)H-Index: 18
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Abstract GnRH is a neuropeptide known to regulate reproduction in vertebrates. The purpose of this study was to design and produce recombinant gonadotropin-releasing hormone associated peptide (rGnRH/GAP) as an alternative of the previous GnRHs and native extracted hormone from tissue, to induce final maturation in fish. Decapeptide as well as GAP area sequences were compared between GnRH1, GnRH2, and mGnRH from Acipenser sp and Huso huso, respectively. Considering the conserved amino acids and ...
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