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Kentaro Shiraki
University of Tsukuba
Organic chemistryChemistryLysozymeAmino acidBiochemistry
231Publications
29H-index
3,341Citations
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Publications 228
Newest
#1Eiko N. MinakawaH-Index: 3
#2Popiel Ha (Osaka University)H-Index: 3
Last. Toshihide Takeuchi (Osaka University)H-Index: 1
view all 27 authors...
: The polyglutamine (polyQ) diseases are a group of inherited neurodegenerative diseases that include Huntington's disease, various spinocerebellar ataxias, spinal and bulbar muscular atrophy, and dentatorubral pallidoluysian atrophy. They are caused by the abnormal expansion of a CAG repeat coding for the polyQ stretch in the causative gene of each disease. The expanded polyQ stretches trigger abnormal β-sheet conformational transition and oligomerization followed by aggregation of the polyQ pr...
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#1Kentaro Shiraki (University of Tsukuba)H-Index: 29
#2Masahiro Mimura (University of Tsukuba)H-Index: 2
Last. Tomoto Ura (University of Tsukuba)H-Index: 1
view all 4 authors...
This review briefly summarizes the effect of additives on the formation of liquid droplets and aggregates of proteins. Proteins have the property of forming liquid droplets and aggregates both in vivo and in vitro. The liquid droplets of proteins are mainly stabilized by electrostatic and cation–π interactions, whereas the amorphous aggregates are mainly stabilized by hydrophobic interactions. Crowders usually stabilize liquid droplets, whereas ions and hexandiols destabilize the droplets. Addit...
1 CitationsSource
#1Shunsuke Tomita (AIST: National Institute of Advanced Industrial Science and Technology)H-Index: 12
#2Hiroka Sugai (AIST: National Institute of Advanced Industrial Science and Technology)H-Index: 1
Last. Ryoji Kurita (AIST: National Institute of Advanced Industrial Science and Technology)H-Index: 2
view all 6 authors...
The detection of proteases and their complexes with inhibitor proteins is of great importance for diagnosis and medical treatment applications. In this study, we report a fingerprint-based sensor using an array of single-stranded DNAs (ssDNAs) labeled with environment-responsive 3’-carboxytetramethylrhodamine (TAMRA) for the identification of proteases. Four TAMRA-modified ssDNAs with different sequences solubilized in two different buffer solutions were incorporated in an array that was capable...
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#1T. MoriH-Index: 20
Last. Seiji KojimaH-Index: 31
view all 11 authors...
Disordered systems exhibit universal excitation, referred to as the boson peak, in the terahertz region. Meanwhile, the so-called fracton is expected to appear in the nanoscale region owing to the self-similar structure of monomers in polymeric glasses. We demonstrate that such excitations can be detected using terahertz spectroscopy. For the interaction between terahertz light and the vibrational density of states of the fractal structure, we formulate an infrared light-vibration coupling coeff...
#1Chika Shibata (University of Tsukuba)H-Index: 1
#2Kazuki Iwashita (University of Tsukuba)H-Index: 6
Last. Kentaro Shiraki (University of Tsukuba)H-Index: 29
view all 3 authors...
Abstract Aggregation of immunoglobulin G (IgG) is a serious concern that results in immunogenicity in pharmaceutical applications. Removal of the small and soluble aggregates in protein solutions through a simple method remains challenging. Here we show that an aqueous two-phase system (ATPS) can be used for the elimination of soluble aggregates from IgG solution. Polyethylene glycol (PEG) and dextran (DEX) were selected as components of the ATPS. As expected, IgG monomers were partitioned into ...
1 CitationsSource
#1T. Mori (University of Tsukuba)H-Index: 20
#2Leona Motoji (University of Tsukuba)
Last. Seiji Kojima (University of Tsukuba)H-Index: 31
view all 8 authors...
Terahertz time-domain spectroscopy has been applied to protein myoglobin dried sample. The boson peak (BP) which is a universal excitation of disordered system has been detected around 0.5 THz. While, fracton will be a universal excitation in the self-similar structure, and it has been observed above the BP frequency. How the fracton appear in the infrared spectrum is explained using the fracton model and the linear response theory for amorphous materials.
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#1Suguru Nishinami (University of Tsukuba)H-Index: 2
#2Tomoshi Kameda (AIST: National Institute of Advanced Industrial Science and Technology)H-Index: 15
Last. Kentaro Shiraki (University of Tsukuba)H-Index: 29
view all 4 authors...
Therapeutic antibodies for subcutaneous (SC) injection must be formulated at high concentrations because of the large therapeutic dose and volume restriction. However, concentrated antibody solutions have undesirably high viscosity, which hampers SC injection. In this study, we demonstrated that hydantoin and its derivatives suppressed the viscosity of concentrated antibody and bovine serum albumin solutions. Hydantoin derivatives, in particular 1-methylhydantoin, appeared more effective. Both h...
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#1Chika Shibata (University of Tsukuba)H-Index: 1
#2Kazuki Iwashita (University of Tsukuba)H-Index: 6
Last. Kentaro Shiraki (University of Tsukuba)H-Index: 29
view all 3 authors...
Abstract Aqueous two-phase system (ATPS) containing salts has been used for protein purification and enrichment. However, it is unclear how proteins are partitioned in the top or bottom phases of the system. In this study, we demonstrate that the partition of proteins in salt-containing ATPS (SATPS) depends only on the relation between the protein-surface amino acids and the type of salt using SATPS. The partition coefficients of four proteins changed depending on the kind of salt, according to ...
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#1Atsushi Hirano (AIST: National Institute of Advanced Industrial Science and Technology)H-Index: 17
#2Kazuki Iwashita (University of Tsukuba)H-Index: 6
Last. Tomoshi Kameda (AIST: National Institute of Advanced Industrial Science and Technology)H-Index: 15
view all 7 authors...
Abstract Salt solutions are widely used as eluents for ion-exchange chromatography. In general, salts reduce the retention of applied solutes on ion-exchange columns via electrostatic screening effects. The reverse phenomenon, namely, salt-enhanced retention, has not been reported. Here, we report that cations, including arginine, guanidine and sodium ions, enhance the retention of uncharged aromatic solutes on a cation-exchange resin, i.e., a negatively charged resin, with carboxyl groups, wher...
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#1Kazuki Iwashita (University of Tsukuba)H-Index: 6
#2Akihiro HandaH-Index: 5
Last. Kentaro Shiraki (University of Tsukuba)H-Index: 29
view all 3 authors...
Abstract Ovotransferrin (OVT) is the main protein component of egg white responsible for initial gelation due to its high thermal susceptibility. Co-aggregation of lysozyme (LYZ) is involved in OVT aggregate formation at low temperatures during pasteurization. Undesirable formation of aggregates limits the degree of thermal processing that can be applied to egg white products. However, the characteristics of co-aggregates of OVT and LYZ have not been elucidated. Here, we determined the thermal c...
1 CitationsSource
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