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Interactions between lactobacilli and chicken macrophages induce antiviral responses against avian influenza virus

Published on Oct 1, 2017in Research in Veterinary Science 1.75
· DOI :10.1016/j.rvsc.2017.10.007
Bahram Shojadoost3
Estimated H-index: 3
(Ontario Veterinary College),
Raveendra R. Kulkarni13
Estimated H-index: 13
(Ontario Veterinary College)
+ 3 AuthorsShayan Sharif35
Estimated H-index: 35
(Ontario Veterinary College)
Cite
Abstract
Abstract Macrophages are an important cell type of the innate immune system that upon activation, can exert antiviral functions and also can induce virus-specific adaptive immune responses. Macrophage interaction with certain probiotic bacteria such as lactobacilli can enhance antiviral functions of these cells. We have previously shown that administration of lactobacilli to chickens can effectively augment immune response to vaccine antigens. Here, we investigated the effects of representative strains of three Lactobacillus species, L. acidophilus , L. reuteri and L. salivarius used alone or in combination, in enhancing antiviral activity of chicken macrophages against avian influenza virus in an in vitro model using MQ-NCSU cells. Treatment of macrophages with probiotic lactobacilli significantly enhanced the antiviral functions, as determined by the virus titration assay. We also found that lactobacilli stimulation of macrophages induced significantly higher expression of interleukin (IL)-1β, interferon (IFN)- γ and IFN-α cytokine genes as well as interferon regulatory factor-7 (IRF7), 2′,5′-oligoadenylate synthetase (OAS) and interferon-inducible transmembrane protein M3 (IFITM3) genes. Furthermore, macrophages that were treated with lactobacilli had significantly enhanced production of nitric oxide (NO) and IFN-γ protein as well as surface expression of the costimulatory molecule CD40. However, the antiviral and immunostimulatory effects of probiotic lactobacilli largely depended on the Lactobacillus species studied. Collectively, the results from our study using an in vitro model showed that certain Lactobacillus species can effectively augment antiviral responses in chicken macrophages.
  • References (47)
  • Citations (2)
Cite
References47
Newest
Published on May 3, 2016in International Reviews of Immunology 3.48
Fiona Long Yan Fong3
Estimated H-index: 3
(Open University of Hong Kong),
Nagendra P. Shah58
Estimated H-index: 58
(HKU: University of Hong Kong)
+ 1 AuthorsHani El-Nezami36
Estimated H-index: 36
(HKU: University of Hong Kong)
Immunomodulation has been shown to be one of the major functions of probiotic bacteria. This review is presented to provide detailed information on the immunomodulatory properties of probiotics in various animal models and clinical practices. Probiotics can regulate helper T (Th) responses and release of cytokines in a strain-specific manner. For example, Lactobacillus rhamnosus GG can induce beneficial Th1 immunomodulatory effect in infants with cow's milk allergy and relieve intestinal inflamm...
Published on Dec 1, 2015in Research in Veterinary Science 1.75
W.M. Quinteiro-Filho11
Estimated H-index: 11
(Ontario Veterinary College),
Jennifer T. Brisbin16
Estimated H-index: 16
(Ontario Veterinary College)
+ 1 AuthorsShayan Sharif35
Estimated H-index: 35
(Ontario Veterinary College)
Lactobacilli are commensal microbes that reside in the intestines of several species, including chickens. Structural constituents of lactobacilli are able to stimulate the host immune system. Macrophages are crucial players in both innate and adaptive immune systems. Here, we investigated the effects of Lactobacillus acidophilus, Lactobacillus reuteri, Lactobacillus salivarius and their cell-free culture supernatants on the pro-inflammatory gene expression profile, nitric oxide (NO) production a...
Published on Nov 2, 2015in Avian Pathology 1.96
Jie-Lun Hu3
Estimated H-index: 3
(AAFC: Agriculture and Agri-Food Canada),
Hai Yu21
Estimated H-index: 21
(AAFC: Agriculture and Agri-Food Canada)
+ 5 AuthorsJoshua Gong13
Estimated H-index: 13
(AAFC: Agriculture and Agri-Food Canada)
Probiotics have been used to control Salmonella colonization in the chicken intestine. Recently, we demonstrated that certain selected Lactobacillus isolates were able to reduce Salmonella infection in the chicken spleen and liver as well as down-regulated Salmonella pathogenicity island 1 virulence gene expression in the chicken caecum. To further understand the mechanisms through which Lactobacillus protected chickens from Salmonella infection, the present study has investigated the Lactobacil...
Published on Jun 1, 2015in Research in Veterinary Science 1.75
Jennifer T. Brisbin16
Estimated H-index: 16
(Ontario Veterinary College),
Lianne Davidge1
Estimated H-index: 1
(Ontario Veterinary College)
+ 1 AuthorsShayan Sharif35
Estimated H-index: 35
(Ontario Veterinary College)
Abstract Lactobacillus acidophilus , Lactobacillus reuteri and Lactobacillus salivarius can influence the adaptive immune responses in chickens but vary in their ability to do so. The present study attempted to identify how these three bacteria alter the innate immune system. A chicken macrophage cell line, MQ-NCSU, was co-cultured with the three live Lactobacillus species, alone or in combination, grown at different temperatures for various durations of time. Late exponential growth phase bacte...
Published on Jun 1, 2015in Research in Veterinary Science 1.75
Bahram Shojadoost3
Estimated H-index: 3
(Ontario Veterinary College),
Shahriar Behboudi19
Estimated H-index: 19
(Institute for Animal Health)
+ 3 AuthorsShayan Sharif7
Estimated H-index: 7
(Ontario Veterinary College)
Abstract Vitamin D 3 is known to modulate both innate and adaptive immune responses in mammals, but there is little information on its effects on avian immune system cells. Here, we studied the effects of vitamin D 3 on chicken macrophages. Chicken macrophages expressed vitamin D receptor (VDR) and lipopolysaccharide (LPS) stimulation increased their VDR expression. Macrophages were treated with 1,25(OH) 2 D 3 in the presence or absence of Toll-like receptor ligands, such as LPS and Pam 3 CSK 4 ...
Published on Aug 28, 2014in PLOS ONE 2.78
Neda Barjesteh10
Estimated H-index: 10
(U of G: University of Guelph),
Shahriar Behboudi19
Estimated H-index: 19
(Institute for Animal Health)
+ 3 AuthorsShayan Sharif35
Estimated H-index: 35
(U of G: University of Guelph)
Chicken macrophages express several receptors for recognition of pathogens, including Toll-like receptors (TLRs). TLRs bind to pathogen-associated molecular patterns (PAMPs) derived from bacterial or viral pathogens leading to the activation of macrophages. Macrophages play a critical role in immunity against viruses, including influenza viruses. The present study was designed to test the hypothesis that treatment of chicken macrophages with TLR ligands reduces avian influenza replication. Furth...
Published on Jan 1, 2014in Frontiers in Immunology 4.72
Sander de Kivit11
Estimated H-index: 11
(Rush University Medical Center),
Mary C. Tobin12
Estimated H-index: 12
(Rush University Medical Center)
+ 2 AuthorsAlan Landay79
Estimated H-index: 79
(UU: Utrecht University)
The intestinal mucosa is constantly facing a high load of antigens including bacterial antigens derived from the microbiota and food. Despite this, the immune cells present in the gastrointestinal tract do not initiate a pro-inflammatory immune response. Toll-like receptors (TLRs) are pattern recognition receptors expressed by various cells in the gastrointestinal tract, including intestinal epithelial cells (IEC) and resident immune cells in the lamina propria. Many diseases, including chronic ...
Published on Mar 1, 2013in Antiviral Research 4.13
Katia E. Garcia-Crespo6
Estimated H-index: 6
(NIH: National Institutes of Health),
Calvin C. Chan2
Estimated H-index: 2
(NIH: National Institutes of Health)
+ 5 AuthorsHelene F. Rosenberg50
Estimated H-index: 50
(NIH: National Institutes of Health)
Abstract We showed previously that wild-type mice primed via intranasal inoculation with live or heat-inactivated Lactobacillus species were fully (100%) protected against the lethal sequelae of infection with the virulent pathogen, pneumonia virus of mice (PVM), a response that is associated with diminished expression of proinflammatory cytokines and diminished virus recovery. We show here that 40% of the mice primed with live Lactobacillus survived when PVM challenge was delayed for 5 months. ...
Published on Nov 16, 2012in Gut microbes
Minja Miettinen20
Estimated H-index: 20
,
Taija E. Pietilä14
Estimated H-index: 14
(UH: University of Helsinki)
+ 6 AuthorsIlkka Julkunen69
Estimated H-index: 69
(National Institute for Health and Welfare)
In this study, we have utilized global gene expression profiling to compare the responses of human primary macrophages to two closely related, well-characterized Lactobacillus rhamnosus strains GG and LC705, since our understanding of the responses elicited by nonpathogenic bacteria in human innate immune system is limited. Macrophages are phagocytic cells of the innate immune system that perform sentinel functions to initiate appropriate responses to surrounding stimuli. Macrophages that reside...
Published on Jul 1, 2012in Journal of Applied Microbiology 2.68
Byoung Joo Seo2
Estimated H-index: 2
(Yeungnam University),
Irfan A. Rather8
Estimated H-index: 8
(Yeungnam University)
+ 4 AuthorsY.H. Park2
Estimated H-index: 2
(Yeungnam University)
Aim: The aims of the study were to isolate anti-H9N2 bacteria from Korean Kimchi isolates and to evaluate its performance in cell line, egg and in specific pathogen-free (SPF) chickens. Methods and Results: Using Madin–Darby canine kidney (MDCK) cell line, 220 bacterial isolates were screened and the isolate YML003 was selected having pronounced antiviral activity against H9N2 virus. This isolate was identified as Leuconostoc mesenteroides by 16S rRNA gene sequencing. Anti-H9N2 activity of the s...
Cited By2
Newest
Published on Mar 6, 2019in Veterinary Research 3.12
Min Feng6
Estimated H-index: 6
(SCAU: South China Agricultural University),
Tingting Xie2
Estimated H-index: 2
(SCAU: South China Agricultural University)
+ 6 AuthorsXiquan Zhang13
Estimated H-index: 13
(SCAU: South China Agricultural University)
Avian leukosis virus subgroup J (ALV-J) infection can cause tumors and immunosuppression in infected chickens. Macrophages play a central role in host defense against invading pathogens. In this study, we discovered an interesting phenomenon: ALV-J replication is weakened from 3 hours post-infection (hpi) to 36 hpi, which was verified using Western blotting and RT-PCR. To further investigate the interaction between ALV-J and macrophages, transcriptome analysis was performed to analyze the host g...
Published on Jan 1, 2019in Veterinary Immunology and Immunopathology 1.85
Bahram Shojadoost3
Estimated H-index: 3
(Ontario Veterinary College),
Raveendra R. Kulkarni13
Estimated H-index: 13
(Ontario Veterinary College)
+ 7 AuthorsShayan Sharif35
Estimated H-index: 35
(Ontario Veterinary College)
Abstract Selenium supplementation in poultry feeds has been known to have beneficial effects on the bird health and performance; however antiviral effects of selenium have remained largely unknown. In this study, we have evaluated the effects of supplementation of chicken diets with organic (Selenium Enriched Yeast; SEY) and inorganic selenium (Sodium Selenite; SS) on low pathogenicity avian influenza virus (H9N2) shedding in the cloacal and oropharyngeal swab samples as well as examined the exp...
Published on Jan 1, 2019
Udayakumar Prithika4
Estimated H-index: 4
(Alagappa University),
Krishnaswamy Balamurugan17
Estimated H-index: 17
(Alagappa University)
Heat Shock Proteins (HSP) are one of the classical molecules that regulate cellular homeostasis. HSP play multifunctional roles that are crucial for folding/unfolding of proteins, cell-cycle control and signaling, and protection of cells against stress/apoptosis. HSP have also been implicated in antigen presentation with the role of chaperoning and transferring antigenic peptides and providing immunity. HSP have been referred as molecular chaperones since they assist in the repair of denatured p...