Enzymatic Production of Non‐Natural Nucleoside‐5′‐Monophosphates by a Thermostable Uracil Phosphoribosyltransferase

The use of enzymes as biocatalysts applied to synthesis of modified nucleoside‐5′‐monophosphates (NMPs) is an interesting alternative to traditional multistep chemical methods which offers several advantages, such as stereo‐, regio‐, and enantioselectivity, simple downstream processing, and mild reaction conditions. Herein we report the recombinant expression, production, and purification of uracil phosphoribosyltransferase from Thermus...