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Jon Del Arco
European University of Madrid
11Publications
4H-index
34Citations
Publications 11
Newest
#1Jon Del ArcoH-Index: 4
#2Alberto Mills (CSIC: Spanish National Research Council)
Last.Jesús Fernández-Lucas (Grupo México)H-Index: 12
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#1Jon Del Arco (UEM: European University of Madrid)H-Index: 4
#2Justin JordaanH-Index: 2
Last.Jesús Fernández-Lucas (Grupo México)H-Index: 12
view all 4 authors...
Abstract The present work aims to develop a magnetic biocatalyst for customized production of nucleoside analogues using mutant His-tagged purine 2’-deoxyribosyltransferase from Trypanosoma brucei ( Tb PDT V11S ) immobilized onto Ni 2+ chelate magnetic iron oxide porous microparticles (M Tb PDT V11S ). Biochemical characterization revealed M Tb PDT V11S 5 as optimal candidate for further studies (10,552 IU g -1 ; retained activity 54 % at 50 °C and pH 6.5). Interestingly, M Tb PDT V11S 5 display...
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#1Jon Del Arco (UEM: European University of Madrid)H-Index: 4
#2Javier Galindo (UEM: European University of Madrid)
Last.Jesús Fernández-Lucas (Grupo México)H-Index: 12
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Abstract Nowadays enzymatic synthesis of nucleic acid derivatives is gaining momentum over traditional chemical synthetic processes. Biotransformations catalyzed by whole cells or enzymes offer an ecofriendly and efficient alternative to the traditional multistep chemical methods, avoiding the use of chemical reagents and organic solvents that are expensive and environmentally harmful. Herein we report for the first time the covalent immobilization a uracil phosphoribosyltransferase (UPRT). In t...
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#1Jon Del Arco (UEM: European University of Madrid)H-Index: 4
#2Elena Pérez (UEM: European University of Madrid)H-Index: 2
Last.Jesús Fernández-Lucas (Grupo México)H-Index: 12
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Abstract The present work describes the functional and structural characterization of adenine phosphoribosyltransferase 2 from Thermus thermophilus HB8 (TtAPRT2). The combination of structural and substrate specificity data provided valuable information for immobilization studies. Dimeric TtAPRT2 was immobilized onto glutaraldehyde-activated MagReSyn®Amine magnetic iron oxide porous microparticles by two different strategies: a) an enzyme immobilization at pH 8.5 to encourage the immobilization ...
2 CitationsSource
#1Jon Del Arco (UEM: European University of Madrid)H-Index: 4
#2Almudena Perona (UEM: European University of Madrid)H-Index: 7
Last.Pedro A. Sánchez-Murcia (University of Vienna)H-Index: 8
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Insight into the catalytic mechanism of Lactobacillus leichmannii nucleoside 2′-deoxyribosyltransferase (LlNDT) has been gained by calculating a quantum mechanics–molecular mechanics (QM/MM) free-energy landscape of the reaction within the enzyme active site. Our results support an oxocarbenium species as the reaction intermediate and thus an SN1 reaction mechanism in this family of bacterial enzymes. Our mechanistic proposal is validated by comparing experimental kinetic data on the impact of t...
Source
#1Jon Del Arco (UEM: European University of Madrid)H-Index: 4
#2Jesús Fernández-Lucas (UEM: European University of Madrid)H-Index: 12
Due to their similarity to natural counterparts, nucleic acid derivatives (nucleobases, nucleosides, and nucleotides, among others) are interesting molecules for pharmaceutical, biomedical, or food industries. For this reason, there is increasing worldwide demand for the development of efficient synthetic processes for these compounds. Chemical synthetic methodologies require numerous protection-deprotection steps and often lead to the presence of undesirable by-products or enantiomeric mixtures...
4 CitationsSource
#1Jon Del Arco (UEM: European University of Madrid)H-Index: 4
#2Pedro A. Sánchez-Murcia (University of Vienna)H-Index: 8
Last.Jesús Fernández-Lucas (UEM: European University of Madrid)H-Index: 12
view all 5 authors...
In our search for thermophilic and acid-tolerant nucleoside 2′-deoxyribosyltransferases (NDTs), we found a good candidate in an enzyme encoded by Chroococcidiopsis thermalis PCC 7203 (CtNDT). Biophysical and biochemical characterization revealed CtNDT as a homotetramer endowed with good activity and stability at both high temperatures (50–100 °C) and a wide range of pH values (from 3 to 7). CtNDT recognizes purine bases and their corresponding 2′-deoxynucleosides but is also proficient using cyt...
4 CitationsSource
#1Jon Del Arco (UEM: European University of Madrid)H-Index: 4
#2María Antonia Martínez (UEM: European University of Madrid)H-Index: 1
Last.Jesús Fernández Lucas (Grupo México)H-Index: 2
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AbstractPurine phosphoribosyltransferases, purine PRTs, are essential enzymes in the purine salvage pathway of living organisms. They are involved in the formation of C-N glycosidic bonds in purine nucleosides-5′-monophosphate (NMPs) through the transfer of the 5-phosphoribosyl group from 5-phospho-α-D-ribosyl-1-pyrophosphate (PRPP) to purine nucleobases in the presence of Mg2+. Herein, we report a simple and thermostable process for the one-pot, one-step synthesis of some purine NMPs using xant...
8 CitationsSource
#1Jon Del ArcoH-Index: 4
Last.Jesús Fernández-LucasH-Index: 12
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The enzymatic synthesis of nucleotides offers several advantages over traditional multistep chemical methods, such as stereoselectivity, regioselectivity, enantioselectivity, simple downstream processing, and the use of mild reaction conditions. However, in order to scale up these bioprocesses, several drawbacks, such as the low enzyme stability and recycling, must be considered. Enzyme immobilization may overcome these cost-related problems by enhancing protein stability and facilitating the se...
5 CitationsSource
#1Jon Del Arco (UEM: European University of Madrid)H-Index: 4
#2Javier Acosta (UEM: European University of Madrid)H-Index: 2
Last.Jesús Fernández-Lucas (Grupo México)H-Index: 12
view all 7 authors...
The use of enzymes as biocatalysts applied to synthesis of modified nucleoside-5'-monophosphates (NMPs) is an interesting alternative to traditional multistep chemical methods which offers several advantages, such as stereo, regio and enantioselectivity, simple downstream processing, and mild reaction conditions. Herein we report the recombinant expression, production and purification of uracil phosphoribosyltransferase from Thermus themophilus HB8 (TtUPRT). The structure of TtUPRT has been dete...
5 CitationsSource
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