Javier Acosta
European University of Madrid
Substrate (chemistry)Organic chemistryEnzymeBiocatalysisBiochemistry
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Publications 2
#1Jon Del Arco (UEM: European University of Madrid)H-Index: 4
#2Javier Acosta (UEM: European University of Madrid)H-Index: 2
Last. Jesús Fernández-Lucas (Grupo México)H-Index: 12
view all 7 authors...
The use of enzymes as biocatalysts applied to synthesis of modified nucleoside-5'-monophosphates (NMPs) is an interesting alternative to traditional multistep chemical methods which offers several advantages, such as stereo, regio and enantioselectivity, simple downstream processing, and mild reaction conditions. Herein we report the recombinant expression, production and purification of uracil phosphoribosyltransferase from Thermus themophilus HB8 (TtUPRT). The structure of TtUPRT has been dete...
5 CitationsSource
#1Javier AcostaH-Index: 2
#2Jon Del ArcoH-Index: 4
Last. Jesús Fernández-LucasH-Index: 12
view all 5 authors...
Biocatalysis reproduce nature’s synthetic strategies in order to synthesize different organic compounds. Natural metabolic pathways usually involve complex networks to support cellular growth and survival. In this regard, multi-enzymatic systems are valuable tools for the production of a wide variety of organic compounds. Methods: The production of different purine nucleosides and nucleoside-5′-monophosphates has been performed for first time, catalyzed by the sequential action of 2′-deoxyribosy...
6 CitationsSource