Original paper
Full-length transcriptome assembly from RNA-Seq data without a reference genome
Abstract
Massively parallel sequencing of cDNA has enabled deep and efficient probing of transcriptomes. Current approaches for transcript reconstruction from such data often rely on aligning reads to a reference genome, and are thus unsuitable for samples with a partial or missing reference genome. Here we present the Trinity method for de novo assembly of full-length transcripts and evaluate it on samples from fission yeast, mouse and whitefly, whose...
Paper Details
Title
Full-length transcriptome assembly from RNA-Seq data without a reference genome
Published Date
May 15, 2011
Journal
Volume
29
Issue
7
Pages
644 - 652
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Notes
History