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#1Joost van Haasteren (California Institute for Quantitative Biosciences)
#2Jie Li (University of California, Berkeley)H-Index: 7
Last. David V. SchafferH-Index: 63
view all 5 authors...
Genome editing has the potential to treat an extensive range of incurable monogenic and complex diseases. In particular, advances in sequence-specific nuclease technologies have dramatically accelerated the development of therapeutic genome editing strategies that are based on either the knockout of disease-causing genes or the repair of endogenous mutated genes. These technologies are progressing into human clinical trials. However, challenges remain before the therapeutic potential of genome e...
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#1Samuele Ferrari (UniSR: Vita-Salute San Raffaele University)H-Index: 2
#2Aurelien JacobH-Index: 2
Last. Federica Cugnata (UniSR: Vita-Salute San Raffaele University)H-Index: 7
view all 14 authors...
Targeted gene editing in hematopoietic stem cells (HSCs) is a promising treatment for several diseases. However, the limited efficiency of homology-directed repair (HDR) in HSCs and the unknown impact of the procedure on clonal composition and dynamics of transplantation have hampered clinical translation. Here, we apply a barcoding strategy to clonal tracking of edited cells (BAR-Seq) and show that editing activates p53, which substantially shrinks the HSC clonal repertoire in hematochimeric mi...
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#1Min Guo (NIH: National Institutes of Health)H-Index: 32
#2Yue Li (ZJU: Zhejiang University)H-Index: 1
Last. Ivan Rey-Suarez (UMD: University of Maryland, College Park)H-Index: 4
view all 29 authors...
The contrast and resolution of images obtained with optical microscopes can be improved by deconvolution and computational fusion of multiple views of the same sample, but these methods are computationally expensive for large datasets. Here we describe theoretical and practical advances in algorithm and software design that result in image processing times that are tenfold to several thousand fold faster than with previous methods. First, we show that an ‘unmatched back projector’ accelerates de...
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#1Renata S. Magalhaes (Wake Forest Institute for Regenerative Medicine)H-Index: 3
#2J. Koudy Williams (Wake Forest Institute for Regenerative Medicine)H-Index: 33
Last. AtalaAnthony (Wake Forest Institute for Regenerative Medicine)H-Index: 107
view all 5 authors...
Bioengineered uterine tissue could provide a treatment option for women with uterine factor infertility. In large animal models, reconstruction of the uterus has been demonstrated only with xenogeneic tissue grafts. Here we use biodegradable polymer scaffolds seeded with autologous cells to restore uterine structure and function in rabbits. Rabbits underwent a subtotal uterine excision and were reconstructed with autologous cell-seeded constructs, with nonseeded scaffolds or by suturing. At 6 mo...
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#1Christopher Wilson (Broad Institute)H-Index: 43
#2Peter J. Chen (Broad Institute)H-Index: 1
Last. David R. Liu (Broad Institute)H-Index: 81
view all 4 authors...
N6-Methyladenosine (m6A) is the most widespread internal messenger RNA modification in humans. Despite recent progress in understanding the biological roles of m6A, the inability to install m6A site specifically in individual transcripts has hampered efforts to elucidate causal relationships between the presence of a specific m6A and phenotypic outcomes. In the present study, we demonstrate that nucleus-localized dCas13 fusions with a truncated METTL3 methyltransferase domain and cytoplasm-local...
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#1Shengxing Wang (CAS: Chinese Academy of Sciences)H-Index: 1
#2Yuan Zong (CAS: Chinese Academy of Sciences)H-Index: 10
Last. Caixia Gao (CAS: Chinese Academy of Sciences)H-Index: 34
view all 9 authors...
Short insertions and deletions can be produced in plant genomes using CRISPR–Cas editors, but reliable production of larger deletions in specific target sites has proven difficult to achieve. We report the development of a series of APOBEC–Cas9 fusion-induced deletion systems (AFIDs) that combine Cas9 with human APOBEC3A (A3A), uracil DNA-glucosidase and apurinic or apyrimidinic site lyase. In rice and wheat, AFID-3 generated deletions from 5′-deaminated C bases to the Cas9-cleavage site. Approx...
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#1Robert Lorenz Chua (Humboldt University of Berlin)H-Index: 3
#2Soeren Lukassen (Humboldt University of Berlin)H-Index: 6
view all 36 authors...
To investigate the immune response and mechanisms associated with severe coronavirus disease 2019 (COVID-19), we performed single-cell RNA sequencing on nasopharyngeal and bronchial samples from 19 clinically well-characterized patients with moderate or critical disease and from five healthy controls. We identified airway epithelial cell types and states vulnerable to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. In patients with COVID-19, epithelial cells showed an ave...
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The University of Washington’s Institute for Protein Design has become a hub to galvanize de novo protein engineering, citizen science and much more since its founding in 2012.
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#1Andrew V. Anzalone (Broad Institute)H-Index: 2
#2Luke W. Koblan (Broad Institute)H-Index: 6
Last. David R. Liu (Broad Institute)H-Index: 81
view all 3 authors...
The development of new CRISPR–Cas genome editing tools continues to drive major advances in the life sciences. Four classes of CRISPR–Cas-derived genome editing agents—nucleases, base editors, transposases/recombinases and prime editors—are currently available for modifying genomes in experimental systems. Some of these agents have also moved rapidly into the clinic. Each tool comes with its own capabilities and limitations, and major efforts have broadened their editing capabilities, expanded t...
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#1Pranam Chatterjee (MIT: Massachusetts Institute of Technology)H-Index: 3
#2Noah Jakimo (MIT: Massachusetts Institute of Technology)H-Index: 3
Last. Erik J. Sontheimer (UMMS: University of Massachusetts Medical School)H-Index: 34
view all 11 authors...
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