Precrystallization aggregation of insulin by dynamic light scattering and comparison with canavalin

The aggregation of insulin prior to crystallization as a rhombohedral crystal of the 2Zn-insulin hexamer was studied by dynamic light scattering (DLS) using photon correlation spectroscopy (PCS). Results indicate that the 2Zn-insulin hexamer does not aggregate into higher oligomers in solution prior to crystallization. It is, therefore, suggested that the hexamer is the crystallizing species, strongly implying that the crystal grows by addition...