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Precrystallization aggregation of insulin by dynamic light scattering and comparison with canavalin

Published on Mar 1, 1991in Solid State Communications1.43
· DOI :10.1016/0022-0248(91)90884-8
Webe Kadima4
Estimated H-index: 4
(UCR: University of California, Riverside),
Alexander McPherson51
Estimated H-index: 51
(UCR: University of California, Riverside)
+ 1 AuthorsFrances Jurnak25
Estimated H-index: 25
(UCR: University of California, Riverside)
Abstract
Abstract The aggregation of insulin prior to crystallization as a rhombohedral crystal of the 2Zn-insulin hexamer was studied by dynamic light scattering (DLS) using photon correlation spectroscopy (PCS). Results indicate that the 2Zn-insulin hexamer does not aggregate into higher oligomers in solution prior to crystallization. It is, therefore, suggested that the hexamer is the crystallizing species, strongly implying that the crystal grows by addition of hexamers to the crystal. This behavior is discussed in terms of the high and specific affinity of zinc coordination to insulin and is compared to the aggregation behavior (measured by DLS-PCS) of the oligomeric protein canavalin prior to crystallization.
  • References (20)
  • Citations (25)
References20
Newest
#1Vincent Mikol (CNRS: Centre national de la recherche scientifique)H-Index: 7
#2Pascale Vincendon (CNRS: Centre national de la recherche scientifique)H-Index: 6
Last.Richard Giegé (CNRS: Centre national de la recherche scientifique)H-Index: 54
view all 5 authors...
#1Webe Kadima (UCR: University of California, Riverside)H-Index: 4
#2Alexander McPherson (UCR: University of California, Riverside)H-Index: 51
Last.Frances Jurnak (UCR: University of California, Riverside)H-Index: 25
view all 4 authors...
#1Vincent Mikol (CNRS: Centre national de la recherche scientifique)H-Index: 7
#2Richard Giegé (CNRS: Centre national de la recherche scientifique)H-Index: 54
#1Edward N. Baker (Massey University)H-Index: 64
#2Tom L. Blundell (Birkbeck, University of London)H-Index: 98
Last.Numminate M. Vijayan (IISc: Indian Institute of Science)H-Index: 2
view all 13 authors...
#1Edward N. Baker (Massey University)H-Index: 64
#2F. R. S. Thomas L. Blundell (Birkbeck, University of London)H-Index: 1
Last.Numminate M. Vijayan (IISc: Indian Institute of Science)H-Index: 2
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Cited By25
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#1Elizabeth L. Forsythe (BAE Systems)H-Index: 1
#2Sridhar Gorti (MSFC: Marshall Space Flight Center)H-Index: 3
Last.Marc L. Pusey (MSFC: Marshall Space Flight Center)H-Index: 26
view all 3 authors...
#1Kenji Waizumi (Yamaguchi University)H-Index: 7
#2Marco Plomp (Radboud University Nijmegen)H-Index: 1
Last.Willem J. P. van Enckevort (Radboud University Nijmegen)H-Index: 23
view all 3 authors...
#1Genpei Li (CAS: Chinese Academy of Sciences)H-Index: 1
#2Jianwei Li (CAS: Chinese Academy of Sciences)H-Index: 1
Last.Da-Cheng Wang (CAS: Chinese Academy of Sciences)H-Index: 27
view all 4 authors...
View next paperOn the crystallization of proteins.