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Alexander McPherson
University of California, Irvine
282Publications
54H-index
11.5kCitations
Publications 282
Newest
Alexander McPherson54
Estimated H-index: 54
(University of California, Irvine),
Steven B. Larson19
Estimated H-index: 19
(University of California, Irvine)
It was found that the crystals of at least a dozen different proteins could be thoroughly stained to an intense color with a panel of dyes. Many, if not most, of the stained protein crystals retained the dyes almost indefinitely when placed in large volumes of dye-free mother liquor. Dialysis experiments showed that most of the dyes that were retained in crystals also bound to the protein when free in solution; less frequently, some dyes bound only in the crystal. The experiments indicated a str...
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Alexander McPherson54
Estimated H-index: 54
,
Lawrence J. DeLucas28
Estimated H-index: 28
5 Citations Download PDF Cite
2015 in Crystallography Reviews [IF: 3.07]
Alexander McPherson54
Estimated H-index: 54
(University of California, Irvine),
Steven B. Larson19
Estimated H-index: 19
(University of California, Irvine)
Determining the structure of an icosahedral virus crystal by X-ray diffraction follows very much the same course as conventional protein crystallography. The major differences arise from the relatively large sizes of the particles, which significantly affect the data collection process, data processing and management, and later, the refinement of a model. Most of the other differences are due to the high 5 3 2 point group symmetry of icosahedral viruses. This alters dramatically the means by whi...
1 Citations Source Cite
Alexander McPherson54
Estimated H-index: 54
(University of California, Irvine),
Jose A. Gavira23
Estimated H-index: 23
(Spanish National Research Council)
Protein crystallization was discovered by chance about 150 years ago and was developed in the late 19th century as a powerful purification tool and as a demonstration of chemical purity. The crystallization of proteins, nucleic acids and large biological complexes, such as viruses, depends on the creation of a solution that is supersaturated in the macromolecule but exhibits conditions that do not significantly perturb its natural state. Supersaturation is produced through the addition of mild p...
78 Citations Download PDF Cite
Alexander McPherson54
Estimated H-index: 54
(University of California, Irvine),
Bob Cudney3
Estimated H-index: 3
© 2014 International Union of Crystallography. For the successful X-ray structure determination of macromolecules, it is first necessary to identify, usually by matrix screening, conditions that yield some sort of crystals. Initial crystals are frequently microcrystals or clusters, and often have unfavorable morphologies or yield poor diffraction intensities. It is therefore generally necessary to improve upon these initial conditions in order to obtain better crystals of sufficient quality for ...
27 Citations Source Cite
Alexander McPherson54
Estimated H-index: 54
(University of California, Irvine),
Yurii G. Kuznetsov16
Estimated H-index: 16
(University of California, Irvine)
The nucleation and growth of protein, nucleic acid and virus crystals from solution are functions of underlying kinetic and thermodynamic parameters that govern the process, and these are all supersaturation-dependent. While the mechanisms of macromolecular crystal growth are essentially the same as for conventional crystals, the underlying parameters are vastly different, in some cases orders of magnitude lower, and this produces very different crystallization processes. Numerous physical featu...
12 Citations Source Cite
Steven B. Larson19
Estimated H-index: 19
(University of California, Irvine),
John Day9
Estimated H-index: 9
(University of California, Irvine),
Alexander McPherson54
Estimated H-index: 54
(University of California, Irvine)
Satellite tobacco mosaic virus (STMV) is among the smallest viruses, having 60 identical subunits arranged with T = 1 icosahedral symmetry. Its crystal structure was solved at 290 K and was refined using, in part, crystals grown in microgravity. Electron-density maps revealed nearly 57% of the genomic ssRNA. Using six flash-cooled crystals, diffraction data were recorded to 1.4 A resolution and independent refinements of the STMV model were carried out versus the previous 1.8 A resolution data r...
7 Citations Source Cite
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