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Alexander McPherson
University of California, Irvine
288Publications
51H-index
10.6kCitations
Publications 288
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#1Steven B. Larson (UCI: University of California, Irvine)H-Index: 22
#2Alexander McPherson (UCI: University of California, Irvine)H-Index: 51
Abstract The β subunit of bovine luteinizing hormone (LH) was crystallized and its structure solved to 3.15 ​A resolution by molecular replacement using human chorionic gonadotropin (hCG) β subunit as search model. The asymmetric unit contains two copies of the β subunit that are related by a non-crystallographic symmetry (NCS) two-fold axis, both copies of which contain proteolytic cleavages after amino acid 100. It is noteworthy that the oligosaccharide moieties covalently attached at asparagi...
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#1Alexander McPherson (UCI: University of California, Irvine)H-Index: 51
#2John W. Day (UCI: University of California, Irvine)H-Index: 60
Last.Steven B. Larson (UCI: University of California, Irvine)H-Index: 22
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In the course of an earlier investigation into the crystallization of proteins based on the addition of intermolecular ligands, the Kunitz type trypsin inhibitor from soybean (SBTI) was crystallized as a complex with 1,5-disulfonylnaphthalene (ligand library 21D). The two molecules within the asymmetric unit of the monoclinic crystals are related by a near-exact NCS 2-fold axis and have essentially the same conformation as was found for them in previous analyses. The protein dimer is maintained ...
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#1Steven B. Larson (UCI: University of California, Irvine)H-Index: 22
#2Jesse A. Jones (UTHSC: University of Tennessee Health Science Center)H-Index: 2
Last.Alexander McPherson (UCI: University of California, Irvine)H-Index: 51
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An iron-containing alcohol dehydrogenase (FeADH) from the hyperthermophilic archaeon Thermococcus thioreducens was crystallized in unit cells belonging to space groups P21, P212121 and P43212, and the crystal structures were solved at 2.4, 2.1 and 1.9 A resolution, respectively, by molecular replacement using the FeADH from Thermotoga maritima (Schwarzenbacher et al., 2004) as a model. In the monoclinic and orthorhombic crystals the dehydrogenase (molecular mass 41.5 kDa) existed as a dimer cont...
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#1Alexander McPherson (UCI: University of California, Irvine)H-Index: 51
#2Steven B. Larson (UCI: University of California, Irvine)H-Index: 22
Human endothelin is a 21-amino-acid polypeptide, constrained by two intra-chain disulfide bridges, that is made by endothelial cells. It is the most potent vasoconstrictor in the body and is crucially important in the regulation of blood pressure. It plays a major role in a host of medical conditions, including hypertension, diabetes, stroke and cancer. Endothelin was crystallized 28 years ago in the putative space group P6122, but the structure was never successfully solved by X-ray diffraction...
2 CitationsSource
#1Alexander McPherson (UCI: University of California, Irvine)H-Index: 51
#2Steven B. Larson (UCI: University of California, Irvine)H-Index: 22
Author(s): McPherson, Alexander; Larson, Steven B | Abstract: Human apolipoprotein C1 (APOC1) is a 57 amino acid long polypeptide that, through its potent inhibition of cholesteryl ester transferase protein, helps regulate the transfer of lipids between lipid particles. We have now determined the structure of APOC1 in four crystal forms by X-ray diffraction. A molecule of APOC1 is a single, slightly bent, α-helix having 13-14 turns and a length of about 80 A. APOC1 exists as a dimer, but the dim...
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#1Alexander McPherson (UCI: University of California, Irvine)H-Index: 51
#2Steven B. Larson (UCI: University of California, Irvine)H-Index: 22
It was found that the crystals of at least a dozen different proteins could be thoroughly stained to an intense color with a panel of dyes. Many, if not most, of the stained protein crystals retained the dyes almost indefinitely when placed in large volumes of dye-free mother liquor. Dialysis experiments showed that most of the dyes that were retained in crystals also bound to the protein when free in solution; less frequently, some dyes bound only in the crystal. The experiments indicated a str...
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#1Debora L. MakinoH-Index: 5
#2John W. DayH-Index: 60
Last.Alexander McPhersonH-Index: 51
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#1Steven B. Larson (UCI: University of California, Irvine)H-Index: 22
#2Alexander McPherson (UCI: University of California, Irvine)H-Index: 51
The Pfp1 protease, a cysteine protease of unknown specificity from the hyperthermophilic archaeon Thermococcus thioreducens, was crystallized in two distinctive crystal forms: from concentrated citrate in one case and PEG in the other. X-ray data were collected from both crystal forms at room temperature to about 1.9 A resolution using a laboratory source and detector, and the structures were solved by molecular replacement using the Pfp1 protease from Pyrococcus horikoshii as the search model. ...
1 CitationsSource
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