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Alexander McPherson
University of California, Irvine
297Publications
53H-index
11.6kCitations
Publications 297
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#1Alexander McPherson (UCI: University of California, Irvine)H-Index: 53
#2John W. Day (UCI: University of California, Irvine)H-Index: 44
Last.Steven B. Larson (UCI: University of California, Irvine)H-Index: 13
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In the course of an earlier investigation into the crystallization of proteins based on the addition of intermolecular ligands, the Kunitz type trypsin inhibitor from soybean (SBTI) was crystallized as a complex with 1,5-disulfonylnaphthalene (ligand library 21D). The two molecules within the asymmetric unit of the monoclinic crystals are related by a near-exact NCS 2-fold axis and have essentially the same conformation as was found for them in previous analyses. The protein dimer is maintained ...
#1Alexander McPherson (UCI: University of California, Irvine)H-Index: 53
#2Steven B. Larson (UCI: University of California, Irvine)H-Index: 13
Human endothelin is a 21-amino-acid polypeptide, constrained by two intra-chain disulfide bridges, that is made by endothelial cells. It is the most potent vasoconstrictor in the body and is crucially important in the regulation of blood pressure. It plays a major role in a host of medical conditions, including hypertension, diabetes, stroke and cancer. Endothelin was crystallized 28 years ago in the putative space group P6122, but the structure was never successfully solved by X-ray diffraction...
#1Alexander McPherson (UCI: University of California, Irvine)H-Index: 53
#2Steven B. Larson (UCI: University of California, Irvine)H-Index: 13
Author(s): McPherson, Alexander; Larson, Steven B | Abstract: Human apolipoprotein C1 (APOC1) is a 57 amino acid long polypeptide that, through its potent inhibition of cholesteryl ester transferase protein, helps regulate the transfer of lipids between lipid particles. We have now determined the structure of APOC1 in four crystal forms by X-ray diffraction. A molecule of APOC1 is a single, slightly bent, α-helix having 13-14 turns and a length of about 80 A. APOC1 exists as a dimer, but the dim...
#1Alexander McPherson (UCI: University of California, Irvine)H-Index: 53
#2Steven B. Larson (UCI: University of California, Irvine)H-Index: 13
It was found that the crystals of at least a dozen different proteins could be thoroughly stained to an intense color with a panel of dyes. Many, if not most, of the stained protein crystals retained the dyes almost indefinitely when placed in large volumes of dye-free mother liquor. Dialysis experiments showed that most of the dyes that were retained in crystals also bound to the protein when free in solution; less frequently, some dyes bound only in the crystal. The experiments indicated a str...
#1Steven B. Larson (UCI: University of California, Irvine)H-Index: 13
#2Alexander McPherson (UCI: University of California, Irvine)H-Index: 53
The Pfp1 protease, a cysteine protease of unknown specificity from the hyperthermophilic archaeon Thermococcus thioreducens, was crystallized in two distinctive crystal forms: from concentrated citrate in one case and PEG in the other. X-ray data were collected from both crystal forms at room temperature to about 1.9 A resolution using a laboratory source and detector, and the structures were solved by molecular replacement using the Pfp1 protease from Pyrococcus horikoshii as the search model. ...
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