Simultaneous purification and immobilization of d-hydantoinase on the immobilized metal affinity membrane via coordination bonds

This study constructs the immobilized metal affinity membrane (IMAM) via coupling of epichlorohydrin, iminodiacetic acid, and nickel ion on the regenerated cellulose membrane. The d-hydantoin-hydrolyzing enzyme (DHTase) harboring a poly-His tagged residue was used as a model protein immobilized on the prepared IMAM. Various immobilization conditions were examined based on the yield of N-carbamoyl-d-p-hydroxyphenylglycine in batch reactions. The...