Biochemical Engineering Journal
Papers 4284
1 page of 429 pages (4,284 results)
Abstract The unconventional yeast Yarrowia lipolytica is known for its capability to synthesize different metabolites of industrial interest such as citric acid (CA). The numerous applications of CA trigger continuous research on improved production systems and substrates costs reduction. In this context, the present contribution explored the production of CA using chosen strains of Y. lipolytica in fermentation media formulated with low-cost and non-seasonal nutrient sources derived from the co...
Abstract To scale-up a filling process in a cell manufacturing system, the impact of the processing time on the final product quality should be evaluated. It is known that the suspended cells in a cryopreservation solution are subjected to various types of stress factors, which eventually results in apoptotic or necrotic death. In this study, the aim was to investigate a critical factor of cell decay in human induced pluripotent stem cells suspended in a cryopreservation solution and to propose ...
Abstract The carbon release behaviour of polylactic acid (PLA)/starch blends and the correlation between solid-phase denitrification (SPD) performance and solid-carbon decomposition were investigated by both batch experiments and numerical simulations. The results showed that the mass ratio of PLA to starch is a key parameter in determining the total organic carbon (TOC) release rate of the blends. Hydrolysis of the blends contributed more markedly to the fraction of TOC released during denitrif...
Abstract Woodchip solid-phase denitrification bioreactors (SPDRs) are widely applied to remove NO3- from water. In this study, two SPDRs with different woodchip filling patterns, namely a gradient filling bioreactor (G-SPDR) and a uniform filling bioreactor (U-SPDR), were constructed and their denitrification performances were investigated. Analysis of the NO3- removal pathway along the water flow path showed that NO3- removal rates could be enhanced using the G-SPDR (35.66-174.55 mg N·L-1·d-1) ...
Abstract A new dynamic model that mimics gastrointestinal function was designed. Elastic and contractile silica gel with gastrointestinal rugae and folds were used to simulate the stomach and large intestine, and silica gel with intestinal folds and villi was utilized to simulate small intestines. Mixing times for Newtonian fluids with Reynolds numbers of 10–5000 and for non-Newtonian fluids with Reynolds numbers of 1–100 were shortened in the new dynamic model bionic gastrointestinal reactor (B...
Abstract Realization of binding reagents that can perform high-fidelity, sequential recognition and detection of amino acids is important for the success of many proposed approaches for single-molecule protein sequencing. Towards this purpose, a variant of the Agrobacterium tumefaciens protein ClpS was previously engineered with improved binding affinity for phenylalanine at the N-terminus of a peptide. In this study, this variant was further engineered for attributes necessary for robust applic...
Abstract A lipase gene was identified and isolated from a fosmid metagenomics library constructed from forest topsoil samples. Error prone PCR and saturation mutagenesis were used to generate seven lipase variants: S69 P/R194Q, S69 G, S69 P, S69E, S69 P/T99S/N190S/R194Q, R194S, and R194A; by testing five different fatty acids of p-nitrophenyl (p-NP) ester, positions S69 and R194 were found to influence the catalytic activity. Specifically, variant R194S was identified that had 5.0, 4.5, 2.1, 2.2...
Abstract The integrated production of biodiesel and biogas has been explored by using N. gaditana, I. galbana and A. platensis as substrate. Three fatty acid ethyl ester (FAEE) production approaches were assessed, namely an indirect process with previously extracted lipids (IPPEL), an indirect process with extracted lipids as free fatty acids (IPELFFA) and a direct process (DP) without a previous lipid extraction stage. Besides de FAEE production, these approaches are considered as pre-treatment...
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Abstract A quantitative analytical method to determine the dead cell ratio of bacteria (death ratio) in sludge was developed for evaluating technologies and determining a sufficient amount of ozone for the sludge ozone process. The method with SYTO9 and propidium iodide as staining agents for live and dead cells, respectively, was optimized. The death ratio developed in this study could successfully predict the reduction ratio of the excess sludge in the sludge ozone process with different ozone...
Abstract Understanding and modeling of the metabolic pathway of a cell by probing the fraction of extracellular metabolites is gaining much interest in biological reactions. This communication deals with metabolic footprinting technique to understand the effect of static- and shake-culture conditions on the production of lovastatin by Monascus purpureus by probing the predominant exometabolites. A strong glycolytic flux pattern was observed in the shake culture of M. purpureus that resulted in t...
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