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Chih-I Chen
National Chung Hsing University
26Publications
11H-index
355Citations
Publications 26
Newest
#1L. P. Lai (NCHU: National Chung Hsing University)H-Index: 1
#2Maw-Rong Lee (NCHU: National Chung Hsing University)H-Index: 3
Last.Tzu-Tai Lee (NCHU: National Chung Hsing University)H-Index: 3
view all 5 authors...
Soybean hulls are a by-product of soybean processing for oil and meal production; Pleurotus eryngii stalk residues (PESR) are by-products of the edible portion of the fruiting body enriched in bioactive metabolites. This study evaluated the effects of co-fermented PESR and soybean hulls with Aureobasidium pullulans on performance and intestinal morphology in broiler chickens. The in vitro experimental results showed that xylananse and mannanase activity of solid-state fermented soybean hulls (10...
5 CitationsSource
#1Chia-Hung Kuo (Wuyi University)H-Index: 17
#2Guan-Jie Chen (NCHU: National Chung Hsing University)H-Index: 3
Last.Chwen-Jen Shieh (NCHU: National Chung Hsing University)H-Index: 25
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Abstract Lipase from Candida rugosa was immobilized on a polyvinylidene fluoride membrane for synthesis of rose flavor ester, 2-phenylethyl acetate. Response surface methodology (RSM) was employed for kinetic modeling of process and prediction the yield. The RSM was used in practice for determining the kinetic models by fitting the initial rate dates based on the equations of ping-pong bi–bi and order bi–bi model. The maximum reaction rate and kinetic constants were matched with the order bi–bi ...
25 CitationsSource
#1Yi-Miao Ko (NCHU: National Chung Hsing University)H-Index: 4
#2Chih-I ChenH-Index: 11
Last.Yung-Chuan Liu (NCHU: National Chung Hsing University)H-Index: 24
view all 9 authors...
Abstract Various immobilized metal ions affinity membranes (IMAMs) were prepared from the regenerated cellulose membrane (RC membrane) and chelated with various metal ions such as Co 2+ , Ni 2+ , Cu 2+ and Zn 2+ . The D-hydantoin-hydrolyzing enzyme (DHTase) harboring a poly-His tagged residue was used as a model protein to be immobilized on the prepared IMAMs through the direct metal–protein interaction forces. The adsorption isotherm and the kinetic parameters V max , K m,app of DHTase on IMAMs...
4 CitationsSource
#1Chia-Chi Lin (NCHU: National Chung Hsing University)H-Index: 5
#2Chih-Hsun Wei (NCHU: National Chung Hsing University)H-Index: 1
Last.Yung-Chuan Liu (NCHU: National Chung Hsing University)H-Index: 24
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Abstract A novel design of a photosynthetic microbial fuel cell (PMFC) is constructed. It is a membrane-less and mediator-free apparatus with a gilding gold mesh as an anode and a graphite carbon cloth as a cathode. The electrode spacing is 4 cm. Spirulina platensis is aggregated and deposited on the anode to form a thin biofilm. Results show that the chlorophyll (Chl) content of the biofilm attributed the PMFC’s electricity performance. The open circuit voltage (OCV) reading of PMFC is proporti...
29 CitationsSource
#1Hua-Bing Chen (NCHU: National Chung Hsing University)H-Index: 4
#2Chih-I ChenH-Index: 11
Last.Yung-Chuan Liu (NCHU: National Chung Hsing University)H-Index: 24
view all 9 authors...
Abstract In this study, Pleurotus eryngii head part waste in a bag-log was collected and processed to yield mushroom hydrolysate powder (MHP). To test its stimulatory effect, a P. eryngii submerged culture was conducted. The prepared MHP (1.6%) and glucose (2%) were found to be the best sources for both mycelial biomass and EPS productions. A specific growth rate of 0.68 day −1 , and a specific EPS yield of 9.55 g/g-dry-cell-weight were obtained under these conditions. It was also found that MHP...
4 CitationsSource
#1Jiun-Yan Wu (NCHU: National Chung Hsing University)H-Index: 4
#2Chih-I ChenH-Index: 11
Last.Yung-Chuan Liu (NCHU: National Chung Hsing University)H-Index: 24
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In this study, three approaches for protein production were devised to enhance the efficiencies in yielding high purity protein via surface display system. A plasmid carrying enhanced green fluorescent protein (EGFP), an intein (INT) and ice nucleation protein (INP) was constructed to produce EGFP via surface display in Escherichia coli. To obtain high purity of the produced EGFP, several procedures, including osmotic shock, surfactant addition and cell disruption were employed. Among these appr...
2 CitationsSource
#1Chi-Yu Chen (NCHU: National Chung Hsing University)H-Index: 5
#2H.-Y. TsenH-Index: 1
Last.Chih-I Chen (NCHU: National Chung Hsing University)H-Index: 11
view all 5 authors...
35 CitationsSource
#1Chih-I Chen (Hsiuping University of Science and Technology)H-Index: 11
#2Yi-Miao Ko (NCHU: National Chung Hsing University)H-Index: 4
Last.Yung-Chuan Liu (NCHU: National Chung Hsing University)H-Index: 24
view all 8 authors...
Abstract In this study, an immobilized metal ion affinity membrane (IMAM) was prepared and used to immobilize penicillin G acylase (PGA). The stability of storage of the immobilized PGA membrane (IPM) was tested. When stored in deionized water (DI) at 4 °C, only 19% residual activity of IPM was retained for 10 days. However, when stored in 10 mM phosphate buffer (PB, pH 8) with 0.1% NaN 3 , the IPM can retain 99% of its activity for a 10-day reaction and storage test. The feasibility of the IPM ...
8 CitationsSource
#1Yi-Miao Ko (NCHU: National Chung Hsing University)H-Index: 4
#2Chih-I ChenH-Index: 11
Last.Yung-Chuan Liu (NCHU: National Chung Hsing University)H-Index: 24
view all 4 authors...
Abstract This study constructs the immobilized metal affinity membrane (IMAM) via coupling of epichlorohydrin, iminodiacetic acid, and nickel ion on the regenerated cellulose membrane. The d -hydantoin-hydrolyzing enzyme (DHTase) harboring a poly-His tagged residue was used as a model protein immobilized on the prepared IMAM. Various immobilization conditions were examined based on the yield of N-carbamoyl- d -p-hydroxyphenylglycine in batch reactions. The immobilization conditions were studied ...
11 CitationsSource
#1Guan-Jie Chen (NCHU: National Chung Hsing University)H-Index: 3
#2Chia-Hung Kuo (NCHU: National Chung Hsing University)H-Index: 17
Last.Yung-Chuan Liu (NCHU: National Chung Hsing University)H-Index: 24
view all 6 authors...
In this study, three membranes: regenerated cellulose (RC), glass fiber (GF) and polyvinylidene fluoride (PVDF), were grafted with 1,4-diaminobutane (DA) and activated with glutaraldehyde (GA) for lipase covalent immobilization. The efficiencies of lipases immobilized on these membranes with different hydrophobic/hydrophilic properties were compared. The lipase immobilized on hydrophobic PVDF-DA-GA membrane exhibited more than an 11-fold increase in activity compared to its immobilization on a h...
27 CitationsSource
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