Match!

Modulation of fibroblast inflammatory response by surface modification of a perfluorinated ionomer

Published on Jun 1, 2011in Biointerphases2.009
· DOI :10.1116/1.3583535
Thelma I. Valdes2
Estimated H-index: 2
(University of Connecticut Health Center),
Winston Ciridon4
Estimated H-index: 4
(UW: University of Washington)
+ 1 AuthorsJames D. Bryers31
Estimated H-index: 31
(UW: University of Washington)
Abstract
An ideal surface for implantable glucose sensors would be able to evade the events leading to chronic inflammation and fibrosis, thereby extending its utility in an in vivo environment. Nafion™, a perfluorinated ionomer, is the membrane material preferred for in situ glucose sensors. Unfortunately, the surface properties of Nafion™ promote random protein adsorption and eventual foreign body encapsulation, thus leading to loss of glucose signal over time. Details of the techniques to render Nafion™ nonprotein fouling are given in a previous article [T. I. Valdes et al., Biomaterials 29, 1356 (2008)]. Once random protein adsorption is prevented, a biologically active peptide can be covalently bonded to the treated Nafion™ to induce cellular adhesion. Cellular responses to these novel decorated Nafion™ surfaces are detailed here, including cell viability, cell spreading, and type I collagen synthesis. Normal human dermal fibroblasts NHDFs) were cultured on control and modified Nafion™ surfaces. Findings indicate that Nafion™ modified with 10% 2-hydroxyethyl methacrylate and 90% tetraglyme created a nonfouling surface that was subsequently decorated with the YRGDS peptide. NHDFs were shown to have exhibited decreased type I collagen production in comparison to NHDF cells on unmodified Nafion™ surfaces. Here, the authors report evidence that proves that optimizing conditions to prevent protein adsorption and enhance cellular adhesion may eliminate fibrous encapsulation of an implant.
  • References (111)
  • Citations (5)
📖 Papers frequently viewed together
19 Citations
2004EMBC: International Conference of the IEEE Engineering in Medicine and Biology Society
8 Citations
78% of Scinapse members use related papers. After signing in, all features are FREE.
References111
Newest
#1Xueyan Zhao (UMN: University of Minnesota)H-Index: 12
#2Hongfei Jia (University of Akron)H-Index: 11
Last. Ping Wang (University of Akron)H-Index: 46
view all 4 authors...
Carbon nanotubes (CNTs) have been used for various bioelectrochemical applications, presumably for substantial improvement in performance. However, often only moderate results observed, with many governing factors have been considered and suggested yet without much systematic evaluation and verification. In this study, CNT-supported glucose oxidase (CNT–GOx) was examined in the presence of 1,4-benzoquinone (BQ). The intrinsic Michaelis parameters of the reaction catalyzed by CNT–GOx were found v...
36 CitationsSource
#1Liuqing Yang (CAS: Chinese Academy of Sciences)H-Index: 6
#2Xiangling Ren (CAS: Chinese Academy of Sciences)H-Index: 26
Last. Lin Zhang (CAS: Chinese Academy of Sciences)H-Index: 51
view all 4 authors...
Abstract A practical glucose biosensor was developed by combining the intrinsic peroxidase-like activity of Fe 3 O 4 nanoparticles (Fe 3 O 4 NPs) and the anti-interference ability of the nafion film. Glucose oxidase (GOD) was simply mixed with Fe 3 O 4 NPs and cross-linked on the Pt electrode with chitosan (Cs) medium by glutaraldehyde, and then covered with a thin nafion film. The biosensor showed high sensitivity (11.54 μA cm −2 mM −1 ), low detection limit (6 × 10 −6 M), and good storage stab...
158 CitationsSource
#1Dan Wen (CAS: Chinese Academy of Sciences)H-Index: 32
#2Xiangqin Zou (CAS: Chinese Academy of Sciences)H-Index: 5
Last. Shaojun Dong (CAS: Chinese Academy of Sciences)H-Index: 101
view all 5 authors...
Abstract Platinum nanoparticles (Pt NPs) were deposited onto multi-walled carbon nanotubes (MWNTs) through direct chemical reduction without any other stabilizing agents. Transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS) and cyclic voltammetry were employed to characterize the morphology of the as-prepared nanocomposite (noted as Pt NPs–MWNTs) and further identify the Pt NPs on the surface of MWNTs. The nanocomposite demonstrated the ability to electrocatalyze the ox...
30 CitationsSource
#1A. I. Gopalan (KNU: Kyungpook National University)H-Index: 7
#2Kwang P. Lee (KNU: Kyungpook National University)H-Index: 1
Last. Jong W. Lee (KNU: Kyungpook National University)H-Index: 1
view all 5 authors...
Abstract A glucose biosensor was fabricated with loading of glucose oxidase (GOx) into a new organic–inorganic hybrid nanocomposite. The preparation involves formation of silica network into a Nafion (perfluorosulfonate ionomer) and subsequent loading of polyaniline grafted multiwalled carbon nanotubes (MWNT-g-PANI) onto Nafion–silica nanocomposite. Field emission scanning electron microscopy (FE-SEM) of Nafion–silica/MWNT-g-PANI composite reveals the presence of spherical silica particles (size...
90 CitationsSource
#1Seong Hwan Kim (ORNL: Oak Ridge National Laboratory)H-Index: 21
#2Touhidur Rahman (ORNL: Oak Ridge National Laboratory)H-Index: 1
Last. Thomas Thundat (ORNL: Oak Ridge National Laboratory)H-Index: 60
view all 5 authors...
Cellulolytic microbes occur in diverse natural niches and are being screened for industrial modification and utility. A microbe for Consolidated bioprocessing (CBP) development can rapidly degrade pure cellulose and then ferment the resulting sugars into fuels. To identify and screen for novel microbes for CBP, we have developed a piezoresistive cantilever array sensor which is capable of simultaneous monitoring of glucose and ethanol concentration changes in a phosphate buffer solution. 4-merca...
10 CitationsSource
#1Ta-Feng Tseng (UCSD: University of California, San Diego)H-Index: 6
#2Yang-Li Yang (UCSD: University of California, San Diego)H-Index: 5
Last. Joseph Wang (UCSD: University of California, San Diego)H-Index: 128
view all 7 authors...
We illustrate how the use of heated electrodes enhances the performance of glucose biosensors based on amperometric detection of the glucose-oxidase generated hydrogen peroxide. Nafion is shown to be an excellent matrix to protect glucose-oxidase from thermal inactivation during the heating pulses. The influence of the electrode temperature upon the amperometric response is examined. Temperature pulse amperometry (TPA) has been used to obtain convenient peak-shaped analytical signals. Surprising...
23 CitationsSource
#1Madalina M. Barsan (UC: University of Coimbra)H-Index: 19
#2Christopher M.A. Brett (UC: University of Coimbra)H-Index: 47
article i nfo A new carbon-based conducting composite has been developed as electrochemical sensor and biosensor for the amperometric detection of ascorbate and glucose. Electrocatalytic oxidation of ascorbate has been done successfully at unmodified cellulose acetate-graphite composite electrodes, the sensor being highly sensitive, selective and with a low detection limit at 0.0 V vs. SCE and was successfully applied for ascorbate determination in commercial fruit juice samples. An interference...
28 CitationsSource
: This work presents a novel approach to design an amperometric transducer capable of detecting glucose and hydrogen peroxide. The biosensor is based on a bienzyme-channelling configuration, employing the enzymes glucose oxidase and horseradish peroxidase, which were immobilized with Toluidine blue (TB) functionalized multiwalled carbon nanotubes (MWNTs). TB was covalently immobilized with carboxylic acid groups of the carbon nanotubes via carbodiimide reaction. The MWNT/TB/GOD/HRP/Nf nanobiocom...
4 CitationsSource
#1Xiaomei Chen (Ministry of Education)H-Index: 28
#2Zhi-min Cai (Ministry of Education)H-Index: 12
Last. Xi Chen (Ha Tai: Xiamen University)H-Index: 51
view all 7 authors...
Abstract A novel non-enzymatic electrochemiluminescence (ECL) sensor based on palladium nanoparticles (PdNPs)–functional carbon nanotubes (FCNTs) was discovered for glucose detection. PdNPs were homogeneously modified on FCNTs using a facile spontaneous redox reaction method. Their morphologies were characterized by transmission electron microscopy (TEM). Based on ECL experimental results, the PdNPs–FCNTs–Nafion film modified electrode displayed high electrocatalytic activity towards the oxidati...
53 CitationsSource
#1Bin Qiu (FZU: Fuzhou University)H-Index: 36
#2Zhenyu Lin (FZU: Fuzhou University)H-Index: 37
Last. Guonan Chen (FZU: Fuzhou University)H-Index: 63
view all 6 authors...
Abstract A poly(nickel(II) tetrasulfophthalocyanine)/multi-walled carbon nanotubes composite modified electrode (polyNiTSPc/MWNTs) was fabricated by electropolymerization of NiTSPc on MWNTs-modified glassy carbon electrode (GCE). The modified electrode was found to be able to greatly improve the emission of luminol electrochemiluminescence (ECL) in a solution containing hydrogen peroxide. Glucose oxidase (GOD) was immobilized on the surface of polyNiTSPc/MWNTs modified GC electrode by Nafion to ...
50 CitationsSource
Cited By5
Newest
#1Qasim Khan (SEU: Southeast University)H-Index: 6
#2Alagesan Subramanian (SEU: Southeast University)H-Index: 1
Last. Qiaoliang Bao (Monash University, Clayton campus)H-Index: 58
view all 10 authors...
2 CitationsSource
#1Volker Schmitt (University of Mainz)H-Index: 10
#2Andreas Mamilos (European Institute)H-Index: 2
Last. Christoph BrochhausenH-Index: 24
view all 11 authors...
: Separating wounded serosa by physical barriers is the only clinically approved adjunct for postoperative adhesion prevention. Since the optimal adhesion barrier has not been found, it is essential to improve our pathogenic understanding of adhesion formation and to compare the effects of different barrier materials on tissue and cells. Wistar rats underwent standardized peritoneal damage and were treated either with Seprafilm, Adept, Intercoat, Spraygel, SupraSeal or remained untreated as a co...
3 CitationsSource
#1Cédric Labay (UPC: Polytechnic University of Catalonia)H-Index: 6
#2J.M. Canal (UPC: Polytechnic University of Catalonia)H-Index: 6
Last. Cristina CanalH-Index: 13
view all 9 authors...
Hernia repair is one of the most common operations in general surgery, and its associated complications typically relate to infections, among others. The loading of antibiotics to surgical meshes to deliver them locally in the abdominal hernia repair site can be one way to manage infections associated with surgical implants. However, the amount of drug loaded is restricted by the low wettability of polypropylene (PP). In this work, plasma has been used to tailor the surface properties of PP mesh...
39 CitationsSource
#1Kim S. Jones (McMaster University)H-Index: 12
The host response to biomaterials is almost always the formation of a fibrous capsule. After implantation of a biomaterial, the body tries to heal the wound by sequentially causing acute inflammation, granulation tissue and the foreign body response, encapsulation by fibrous tissue, and capsular contracture. These responses are detrimental to many applications of biomaterials. Fibrosis is initiated by surface properties, mechanical signals, and changes in microenvironment. In fibrotic disease, t...
5 CitationsSource
#2Yan WangH-Index: 16
view all 5 authors...
Biofouling and tissue inflammation present major challenges toward the realization of long-term implantable glucose sensors. Following sensor implantation, proteins and cells adsorb on sensor surfaces to not only inhibit glucose flux but also signal a cascade of inflammatory events that eventually lead to permeability-reducing fibrotic encapsulation. The use of drug-eluting hydrogels as outer sensor coatings has shown considerable promise to mitigate these problems via the localized delivery of ...
22 CitationsSource