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Pathogenicity and molecular characteristics of infectious bronchitis virus (IBV) strains isolated from broilers showing diarrhoea and respiratory disease

Published on May 4, 2014in British Poultry Science1.42
· DOI :10.1080/00071668.2014.903558
Jorge Luis Chacón10
Estimated H-index: 10
(USP: University of São Paulo),
M. S. Assayag3
Estimated H-index: 3
(USP: University of São Paulo)
+ 4 AuthorsA. J. Piantino Ferreira6
Estimated H-index: 6
(USP: University of São Paulo)
Cite
Abstract
Abstract1. The possibility that infectious bronchitis virus (IBV) variants isolated from broilers with enteric and respiratory problems have a different tropism and pathological outcome from those IBV strains causing classical respiratory disease was investigated.2. IBV variants were isolated from broiler flocks with enteric and respiratory problems in two regions of Brazil. The USP-10 isolate, of enteric origin, was inoculated via the oral oroculonasal routes into IBV-antibody-free broilers and specific pathogen-free (SPF) chickens to determine tissue tropism and pathogenicity and compared with an IBV variant (USP-50) isolated from chickens showing signs of respiratory disease only.3. Both USP-10 and USP-50 strains caused similar pathological patterns by either route of inoculation. Both variants were detected in respiratory and non-respiratory tissues, including the kidney, intestine and testis.4. Broilers were more susceptible to infection than SPF chickens, and seroconversion was detected in all of th...
  • References (43)
  • Citations (10)
Cite
References43
Newest
Published on Jan 1, 2015
Radha1
Estimated H-index: 1
Demography-hitherto a problem area for India due to over population has become a storehouse of opportunities as its age pyramid bulges in the age group of 15–59 years with 64% of its population in this group by the year 2022. India is expected to have 28% of world's workforce, a billion person labour force out of approximate population of 1.4 billion citizens, with average age of 29 years. This demographic advantage has to be transformed into economic advantage by absorbing the young population ...
Published on Jun 1, 2011in Avian Pathology1.96
J. J. de Wit16
Estimated H-index: 16
,
Jane K. A. Cook31
Estimated H-index: 31
,
Harold M. J. F. van der Heijden10
Estimated H-index: 10
The history, current situation and control measures for infectious bronchitis virus (IBV) variants are reviewed. A large number of IBV variants exist worldwide; some being unique to a particular area, others having a more general distribution. The possible reasons why some strains spread readily over major parts of the world, whereas other strains stay more localized are discussed. The advantages and disadvantages of strain classification by protectotyping, serotyping and genotyping are discusse...
Published on Jun 1, 2011in Avian Diseases1.31
Rodrigo A. Gallardo8
Estimated H-index: 8
(AU: Auburn University),
F. J. Hoerr13
Estimated H-index: 13
+ 2 AuthorsHaroldo Toro18
Estimated H-index: 18
(AU: Auburn University)
Abstract Even though males represent only 8%–12% of the birds of a breeder flock, their role in infectious bronchitis virus (IBV) dissemination is largely unknown. We first assessed the effect of IBV replication in the chicken testes. Ten-week-old males were inoculated with Arkansas (Ark) or Massachusetts (Mass) IBV virulent strains. Seven days postinoculation (DPI) IBV RNA was detected in testicles of 100% of M41- and in 96% of Ark-infected males. Marginal nonsynonymous variation was detected i...
Published on Apr 1, 2011in Avian Pathology1.96
Jorge Luis Chacón10
Estimated H-index: 10
(USP: University of São Paulo),
Juliana Nogueira Rodrigues2
Estimated H-index: 2
(USP: University of São Paulo)
+ 3 AuthorsAntonio José Piantino Ferreira16
Estimated H-index: 16
(USP: University of São Paulo)
As part of an epidemiological study of infectious bronchitis virus (IBV) in Brazil, 252 samples from IBV-suspect flocks were tested and the IBV-positive samples were analysed by sequencing of hypervariable regions 1 and 2 of the S1 gene. A high prevalence of IBV variants was found and the sequence analysis of 41 samples revealed a high molecular similarity among the Brazilian isolates (from 90.2 to 100% and from 85.3 to 100% nucleotide and amino acid identity, respectively). The Brazilian isolat...
Published on Dec 1, 2010in Avian Diseases1.31
P. A. N. Felippe7
Estimated H-index: 7
(State University of Campinas),
L. H. A. da Silva1
Estimated H-index: 1
(State University of Campinas)
+ 2 AuthorsClarice Weiss Arns4
Estimated H-index: 4
(State University of Campinas)
Abstract To detect the presence of infectious bronchitis virus or avian coronavirus, a nested reverse transcriptase PCR (RT-PCR) method was developed with the aim of amplifying a fragment of 530 bases, comprising the gene coding S1 protein. In the first step, all samples were submitted to RNA extraction, RT-PCR, and nested PCR. Next, only the positive nested-PCR samples were propagated in specific-pathogen-free (SPF) embryonated chicken eggs for virus isolation. Positive samples were then sequen...
Published on Nov 1, 2010in Journal of Comparative Pathology1.25
Zs. Benyeda1
Estimated H-index: 1
(Szent István University),
L. Szeredi1
Estimated H-index: 1
+ 5 AuthorsVilmos Palya21
Estimated H-index: 21
(CEVA Logistics)
Summary The aim of this study was to compare experimentally the pathogenicity and tissue distribution of the recently emerged QX-like strain of infectious bronchitis virus (IBV) with the widespread M41 and 793/B serotypes of the virus. Histopathological and immunohistochemical methods were employed to define the main sites of virus replication. One-day-old specific pathogen free chickens were inoculated with five different QX-like strains, or with the M41 and 793/B IBV strains and monitored for ...
Published on Dec 1, 2009in Avian Pathology1.96
Zsófia Benyeda4
Estimated H-index: 4
(Szent István University),
Tamás Mató14
Estimated H-index: 14
(CEVA Logistics)
+ 5 AuthorsVilmos Palya21
Estimated H-index: 21
(CEVA Logistics)
Five QX-like infectious bronchitis virus (IBV) strains, isolated from different field outbreaks and two reference IBV strains of known serotypes (M41 and 793/B), were used in the present study to investigate and compare their pathogenicity for 1-day-old specific pathogen free chickens. The ability of the strains to inhibit trachea epithelium ciliary activity, to induce immune response, to replicate and to cause histopathological lesions in designated organs was followed by repeated samplings dur...
Published on Jun 1, 2008in Avian Pathology1.96
K. J. Worthington10
Estimated H-index: 10
(University of Liverpool),
R. J. W. Currie1
Estimated H-index: 1
,
R. C. Jones35
Estimated H-index: 35
(University of Liverpool)
A survey of infectious bronchitis virus (IBV) genotypes in poultry flocks in selected countries in Western Europe was carried out between March 2002 and December 2006. Identification of IBV was by reverse transcriptase-polymerase chain reaction of RNA extracted from oropharyngeal swabs taken from poultry flocks exhibiting signs of clinical disease thought to be attributable to IBV. Part of the hypervariable S1 gene of IBV was sequenced to differentiate between the various genotypes. During the s...
Published on Dec 1, 2007in Avian Diseases1.31
L. Y. B. Villarreal7
Estimated H-index: 7
,
Paulo Eduardo Brandão18
Estimated H-index: 18
(USP: University of São Paulo)
+ 6 AuthorsAntonio José Piantino Ferreira16
Estimated H-index: 16
Abstract The pathogenesis of infection involving both infectious bronchitis virus (IBV) and avian metapneumovirus (aMPV) causes reproductive damage in hens after viral replication in the epithelium of the oviduct, resulting in loss of cilia and degeneration and necrosis of the epithelial and glandular cells. Although IBV has been indicated as a possible cause of the formation of calcium stones in the epididymus of roosters, a definitive association has not been confirmed. This report describes t...
Published on Dec 1, 2007in Avian Diseases1.31
L. Y. B. Villarreal7
Estimated H-index: 7
,
Paulo Eduardo Brandão18
Estimated H-index: 18
(USP: University of São Paulo)
+ 4 AuthorsA. J. P. Ferreira13
Estimated H-index: 13
Abstract Infectious bronchitis virus (IBV) is the causative agent of avian infectious bronchitis, which is characterized by respiratory, reproductive, and renal signs. However, the role of IBV as an enteric pathogen in still controversial. In Brazil, antigenic groups of IBV divergent from the Massachusetts serotype used for vaccination schedules in that country have already been demonstrated. The present study aimed to assess the different genotypes of IBV in Brazilian commercial poultry flocks ...
Cited By10
Newest
Abdelfattah H. Eladl3
Estimated H-index: 3
,
Nagah Arafat2
Estimated H-index: 2
+ 3 AuthorsWalaa Awadin5
Estimated H-index: 5
Abstract Avian influenza vaccines are commonly used in the poultry industry, and some medicinal plants can increase the efficacy of such vaccines. The objective of this study was to evaluate the effect of Immulant ® (IMU) (a commercial product based on Echinacea and Nigella sativa ) on stress induced by dexamethasone (DEX) in chickens vaccinated (VAC) against the H9N2 avian influenza virus (AIV-H9N2). Seven experimental groups were included: the negative control, VAC, DEX, VAC + DEX, VAC + DEX +...
Published on Aug 30, 2018
Paulo Eduardo Brandão18
Estimated H-index: 18
(USP: University of São Paulo),
Aline Santana da Hora4
Estimated H-index: 4
+ 2 AuthorsSueli Akemi Taniwaki5
Estimated H-index: 5
(USP: University of São Paulo)
ABSTRACT Avian coronavirus, the causative agent of avian infectious bronchitis, occurs as multiple genotypes and lineages, and full genomes are not available for the majority of them. This paper reports the (previously unknown) complete genome sequence of strain D274 of this virus (27,599 nucleotides), isolated from chickens in The Netherlands in 1979.
Published on May 1, 2018in Journal of Comparative Pathology1.25
Nguyen Phuc Khanh1
Estimated H-index: 1
(UPM: Universiti Putra Malaysia),
Sheau Wei Tan12
Estimated H-index: 12
(UPM: Universiti Putra Malaysia)
+ 5 AuthorsAbdul Rahman Omar26
Estimated H-index: 26
(UPM: Universiti Putra Malaysia)
Summary Infectious bronchitis viruses (IBVs) circulating in Malaysia are classified into two groups as Malaysian QX-like and variant strains. In this study, the pathogenicity of IBS130/2015 (QX-like) and IBS037A/2014 (variant) IBVs in 1-day-old and 30-day-old specific pathogen free (SPF) chickens was characterized. Both strains caused respiratory and kidney infections based on immunohistochemistry (IHC), real-time quantitative polymerase chain reaction (qPCR) and a ciliostasis study; however, th...
Published on Nov 2, 2017in British Poultry Science1.42
Claudia Carranza3
Estimated H-index: 3
(USP: University of São Paulo),
Claudete S. Astolfi-Ferreira7
Estimated H-index: 7
(USP: University of São Paulo)
+ 6 AuthorsAntonio José Piantino Ferreira16
Estimated H-index: 16
(USP: University of São Paulo)
1. Infectious bronchitis virus (IBV) variants in Brazil were isolated during 2010-2015 for epidemiological and molecular analysis to characterise the different variants and perform a bioinformatic analysis to compare with sequences of variants collected over the previous 40 years. 2. Of the 453 samples examined, 61.4% were positive for IBV and 75.9% of these were considered to have the BR-I genotype and were detected in birds of all ages distributed in all five Brazilian regions. 3. The ratio of...
Published on Sep 22, 2017in International Journal of Molecular Sciences4.18
Shu-Yi Lin3
Estimated H-index: 3
,
Hui-Wen Chen8
Estimated H-index: 8
Infectious bronchitis virus (IBV) variants constantly emerge and pose economic threats to poultry farms worldwide. Numerous studies on the molecular and pathogenic characterization of IBV variants have been performed between 2007 and 2017, which we have reviewed herein. We noted that viral genetic mutations and recombination events commonly gave rise to distinct IBV genotypes, serotypes and pathotypes. In addition to characterizing the S1 genes, full viral genomic sequencing, comprehensive antig...
Published on Dec 1, 2016in BMC Veterinary Research1.79
Khadija Khataby4
Estimated H-index: 4
,
Faouzi Kichou1
Estimated H-index: 1
+ 1 AuthorsMy Mustapha Ennaji14
Estimated H-index: 14
Background Avian infectious bronchitis (IB) is one of the most important viral diseases of poultry, affecting chickens of all ages and causing major economic losses in poultry flocks. Mass vaccination is conducted in Morocco using a vaccine against Massachusetts, which is the most dominant serotype; however no information about the pathogenesis and tissue distribution of the Moroccan Italy 02 genotype was reported. 40 one-day-old specific pathogen free chickens were divided randomly into four gr...
Published on Jun 1, 2016in Avian Diseases1.31
Rüdiger Hauck3
Estimated H-index: 3
(UC Davis: University of California, Davis),
Rodrigo A. Gallardo8
Estimated H-index: 8
(UC Davis: University of California, Davis)
+ 1 AuthorsH. L. Shivaprasad2
Estimated H-index: 2
(University of California, Berkeley)
SUMMARY Runting stunting syndrome (RSS) is a disease condition that affects broilers and causes impaired growth and poor feed conversion because of enteritis characterized by pale and distended small intestines with watery contents. The etiology of the disease is multifactorial, and a large variety of viral agents have been implicated. Here we describe the detection and isolation of an infectious bronchitis virus (IBV) -like coronavirus from the intestines of a flock of 60,000 14-day-old brown/r...
Published on Apr 1, 2016in Research in Veterinary Science1.75
Faez Awad6
Estimated H-index: 6
(University of Liverpool),
Rajesh Chhabra5
Estimated H-index: 5
(University of Liverpool)
+ 5 AuthorsKannan Ganapathy12
Estimated H-index: 12
(University of Liverpool)
Pathogenesis of an IS/885/00-like (IS/885) strain of variant infectious bronchitis virus (IBV) was examined in one day old specific pathogen free (SPF) and commercial broiler chicks. Chicks were humanely euthanized at 3, 6, 9, 12, 15, 21 and 28 days post infection (dpi) for necropsy examination, and tissues were collected for histopathology and virus detection by reverse transcription polymerase chain reaction (RT-PCR). Respiratory clinical signs and gross lesions consisting of tracheal caseous ...
Published on Apr 1, 2016in Infection, Genetics and Evolution2.61
Viviana Valastro9
Estimated H-index: 9
(UNIPD: University of Padua),
Edward C. Holmes109
Estimated H-index: 109
(USYD: University of Sydney)
+ 4 AuthorsIsabella Monne24
Estimated H-index: 24
Infectious bronchitis virus (IBV) is the causative agent of a highly contagious disease that results in severe economic losses to the global poultry industry. The virus exists in a wide variety of genetically distinct viral types, and both phylogenetic analysis and measures of pairwise similarity among nucleotide or amino acid sequences have been used to classify IBV strains. However, there is currently no consensus on the method by which IBV sequences should be compared, and heterogeneous genet...
Published on Jan 30, 2015in PLOS ONE2.78
J. Michael Day10
Estimated H-index: 10
(USDA: United States Department of Agriculture),
Brian B. Oakley24
Estimated H-index: 24
(USDA: United States Department of Agriculture)
+ 1 AuthorsL. Zsak28
Estimated H-index: 28
(USDA: United States Department of Agriculture)
There is a great deal of interest in characterizing the complex microbial communities in the poultry gut, and in understanding the effects of these dynamic communities on poultry performance, disease status, animal welfare, and microbes with human health significance. Investigations characterizing the poultry enteric virome have identified novel poultry viruses, but the roles these viruses play in disease and performance problems have yet to be fully characterized. The complex bacterial communit...