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Perspectives on protein crystallisation

Published on Nov 1, 2009in Progress in Biophysics & Molecular Biology2.703
· DOI :10.1016/j.pbiomolbio.2009.12.001
Takashi Ochi13
Estimated H-index: 13
(University of Cambridge),
Victor M. Bolanos-Garcia24
Estimated H-index: 24
(University of Cambridge)
+ 1 AuthorsAbel Moreno3
Estimated H-index: 3
(University of Cambridge)
Abstract
This final part on 'perspectives' is focused on new strategies that can be used to crystallise proteins and improve the crystal quality of macromolecular complexes using any of the methods reviewed in this focused issue. Some advantages and disadvantages, limitations, and plausible applications to high-resolution X-ray crystallography are discussed.
  • References (96)
  • Citations (13)
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The relative merits and domains of application of three observation techniques (atomic force microscopy, Michelson interferometry, and laser confocal microscopy with differential interference contrast microscopy) for the investigation of crystal growth kinetics are discussed in the context of protein crystallization. Growth rate measurements on the same system under identical experimental conditions using different techniques show differences up to 5-fold in growth rate and a different behavior ...
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