All-Optical Analysis of TRPC3/6 Signalling in Mast Cells

Transient receptor potential canonical channels TRPC3/6 are expressed in various cell types of the immune system. These Ca2+ permeable channels are typically activated downstream of the phospholipase C (PLC) pathway, but their role in immune cell Ca2+ handling is still elusive. We set out to explore the impact of TRPC3 and TRPC6 function in RBL mast cells at the level of local, sub-plasmalemmal and global cellular Ca2+ signalling, independently...