Biophysical Journal
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#1Besian I. Sejdiu (U of C: University of Calgary)H-Index: 2
#2D. Peter Tieleman (U of C: University of Calgary)H-Index: 59
Abstract G Protein-Coupled Receptors (GPCRs) are membrane-bound proteins that depend on their lipid environment to carry out their physiological function. Combined efforts from many theoretical and experimental studies on the lipid-protein interaction profile of several GPCRs hint at an intricate relationship of these receptors with their surrounding membrane environment, with several lipids emerging as particularly important. Using coarse-grained molecular dynamics simulations, we explore the l...
#1Andrea C. Mendoza (McGill University)
#2Dilson E. Rassier (McGill University)H-Index: 25
Abstract It has been accepted that the force produced by a skeletal muscle myofibril depends on its cross-sectional area, but not on the number of active sarcomeres since they are arranged in series. However, a previous study performed by our group showed that blocking actomyosin interactions within an activated myofibril and depleting the thick filaments in one sarcomere unexpectedly reduced force production. In this study we examined in detail how consecutive depletion of thick filaments in in...
#1Davide Tavella (UMMS: University of Massachusetts Medical School)H-Index: 1
#2Asli Ertekin (UMMS: University of Massachusetts Medical School)
Last. Francesca Massi (UMMS: University of Massachusetts Medical School)H-Index: 15
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Abstract CCCH-type tandem zinc finger (TZF) domains are found in many RNA-binding proteins (RBPs) that regulate the essential processes of post-transcriptional gene expression and splicing through direct protein-RNA interactions. In Caenorhabditis elegans, RBPs control the translation, stability, or localization of maternal mRNAs required for patterning decisions prior to zygotic gene activation. MEX-5 is a C. elegans protein that leads a cascade of RBP localization events that is essential for ...
#1Maximilian Jakobs (University of Cambridge)H-Index: 3
#2Kristian Franze (University of Cambridge)H-Index: 28
Last. Assaf Zemel (HUJI: Hebrew University of Jerusalem)H-Index: 16
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Abstract The densely packed microtubule (MT) array found in neuronal cell projections (neurites) serves two fundamental functions simultaneously: It provides a mechanically stable track for molecular-motor based transport, and produces forces which drive neurite growth. The local pattern of MT polarity along the neurite shaft has been found to differ between axons and dendrites. In axons, the neurons’ dominating long projections, roughly 90% of the MTs orient with their rapidly growing plus-end ...
#1Aleander Rohrbach (University of Freiburg)
#2Tim Meyer (University of Freiburg)
Last. Holger Kress (University of Bayreuth)H-Index: 12
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Abstract Thermal motions enable a particle to probe the optimal interaction state when binding to a cell membrane. However, especially on the scale of microseconds and nanometers, position and orientation fluctuations are difficult to observe with common measurement technologies. Here we show that it is possible to detect single binding events of IgG-coated polystyrene beads, which are held in an optical trap nearby the cell membrane of a macrophage. Changes in the spatial and temporal thermal f...
#1Michael Bugiel (University of Tübingen)H-Index: 5
#2Mayank Chugh (University of Tübingen)H-Index: 2
Last. Anita Jannasch (University of Tübingen)H-Index: 8
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Abstract Microtubules are highly dynamic filaments with dramatic structural rearrangements and length changes during the cell cycle. An accurate control of the microtubule length is essential for many cellular processes, in particular during cell division. Motor proteins from the kinesin-8 family depolymerize microtubules by interacting with their ends in a collective and length-dependent manner. However, it is still unclear how kinesin-8 depolymerizes microtubules. Here, we tracked the microtub...
#1Bibiana Onoa (University of California, Berkeley)H-Index: 5
#2Shingo Fukuda (University of California, Berkeley)H-Index: 1
Last. Krishna K. Niyogi (University of California, Berkeley)H-Index: 60
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Abstract Thylakoid membranes in chloroplasts contain photosynthetic protein complexes that convert light energy into chemical energy. Photosynthetic protein complexes are considered to undergo structural reorganization to maintain the efficiency of photochemical reactions. A detailed description of the mobility of photosynthetic complexes in real-time is necessary to understand how macromolecular organization of the membrane is altered by environmental fluctuations. Here, we used high-speed atom...
#1Noah R. Cohen (UMMS: University of Massachusetts Medical School)H-Index: 1
#2Can Kayatekin (UMMS: University of Massachusetts Medical School)H-Index: 13
Last. C. R. Matthews (UMMS: University of Massachusetts Medical School)H-Index: 4
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Abstract The folding reaction of a stable monomeric variant of Cu/Zn superoxide dismutase (mSOD1), an enzyme responsible for the conversion of superoxide free radicals into hydrogen peroxide and oxygen, is among the slowest known folding processes that adhere to two-state behavior. The long lifetime, ∼10 s, of the unfolded state presents ample opportunities for the polypeptide chain to transiently sample nonnative structures prior to formation of the productive folding transition state. We recen...
#1Christopher A. Reissaus (IU: Indiana University)H-Index: 2
#2Kathleen H. Day (IU: Indiana University)H-Index: 3
Last. Richard N. Day (IU: Indiana University)H-Index: 36
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Abstract We report the use of pulsed interleaved excitation-fluorescence lifetime imaging microscopy (PIE-FLIM) to measure the activities of two different biosensor probes simultaneously in single living cells. Many genetically encoded biosensors rely on the measurement of Forster resonance energy transfer (FRET) to detect changes in biosensor conformation that accompany the targeted cell signaling event. One of the most robust ways of quantifying FRET is to measure changes in the fluorescence l...
#1Haipei Liu (HKU: University of Hong Kong)H-Index: 1
#2Chao Fang (HKU: University of Hong Kong)H-Index: 2
Last. Yuan Lin (HKU: University of Hong Kong)H-Index: 15
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Abstract A current bottleneck in the advance of neurophysics is the lack of reliable methods to quantitatively measure the interactions between neural cells and their microenvironment. Here, we present an experimental technique to probe the fundamental characteristics of neuron adhesion through repeated peeling of well-developed neurite branches on a substrate with an AFM cantilever. At the same time, a total internal reflection fluorescence microscope (TIRFM) is also used to monitor the activit...
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