Biophysical Journal
Papers 62160
1 page of 6,216 pages (62.2k results)
Published on 2019in Biophysical Journal3.67
J.A. Mosier (Vandy: Vanderbilt University), Aniqua Rahman-Zaman (Vandy: Vanderbilt University)+ 4 AuthorsCynthia A. Reinhart-King36
Estimated H-index: 36
(Vandy: Vanderbilt University)
Abstract During metastasis, cancer cells navigate through a spatially heterogeneous extracellular matrix (ECM). Physical properties of ECM, including the degree of confinement, influence cell migration behavior. Here, utilizing in vitro three-dimensional (3D) collagen microtracks, we demonstrate that cell-ECM interactions, specifically the degree of spatial confinement, regulate migratory behavior. We found that cells migrate faster when they are fully confined, contacting all four walls (top, b...
Published on 2019in Biophysical Journal3.67
Anagha Datar1
Estimated H-index: 1
(RRI: Raman Research Institute),
Jaishabanu Ameeramja1
Estimated H-index: 1
(RRI: Raman Research Institute)
+ 6 AuthorsPramod A. Pullarkat12
Estimated H-index: 12
(RRI: Raman Research Institute)
Abstract Axonal beading—formation of a series of swellings along the axon—and retraction are commonly observed shape transformations that precede axonal atrophy in Alzheimer’s, Parkinson, and other neurodegenerative conditions. The mechanisms driving these morphological transformations are poorly understood. Here we report controlled experiments which can induce either beading or retraction and follow the time evolution of these responses. By making quantitative analysis of the shape modes under...
Published on 2019in Biophysical Journal3.67
Changmiao Guo4
Estimated H-index: 4
(UD: University of Delaware),
J. C. Williams (Beckman Research Institute), Tatyana Polenova31
Estimated H-index: 31
(UD: University of Delaware)
Abstract Microtubule-associated proteins (MAPs) perform diverse functions in cells. These functions are dependent on their interactions with microtubules. Dynactin, a cofactor of dynein motor, assists the binding of dynein to various organelles and is crucial to the long-distance processivity of dynein-based complexes. The largest subunit of dynactin, the p150glued, contains a N-terminus segment that is responsible for the microtubule-binding interactions and long-range processivity of dynactin....
Published on Jul 1, 2019in Biophysical Journal3.67
Amir R. Khan22
Estimated H-index: 22
(Trinity College, Dublin),
Susan James3
Estimated H-index: 3
(MU: Maynooth University)
+ 3 AuthorsJennifer J. McManus13
Estimated H-index: 13
(MU: Maynooth University)
Abstract Protein crystal production is a major bottleneck in the structural characterisation of proteins. To advance beyond large-scale screening, rational strategies for protein crystallization are crucial. Understanding how chemical anisotropy (or patchiness) of the protein surface due to the variety of amino acid side chains in contact with solvent, contributes to protein-protein contact formation in the crystal lattice is a major obstacle to predicting and optimising crystallization. The rel...
Published on 2019in Biophysical Journal3.67
Sankalp Shukla (UPenn: University of Pennsylvania), Rui Jin1
Estimated H-index: 1
(UPenn: University of Pennsylvania)
+ 2 AuthorsTobias Baumgart31
Estimated H-index: 31
(UPenn: University of Pennsylvania)
Abstract Phosphatidylinositol-4,5-bisphosphate [PIP2] is an important signaling lipid in eukaryotic cell plasma membranes, playing an essential role in diverse cellular processes. The headgroup of PIP2 is highly negatively charged and this lipid displays a high critical micellar concentration compared to housekeeping phospholipid analogs. Given the crucial role of PIP2, it is imperative to study its localization, interaction with proteins, and membrane shaping properties. Biomimetic membranes ha...
Published on Sep 1, 2019in Biophysical Journal3.67
Lindsey N Miller (UT: University of Tennessee), William T Brewer + 2 AuthorsTessa R. Calhoun12
Estimated H-index: 12
(UT: University of Tennessee)
Abstract Bacterial membranes are complex mixtures with dispersity that is dynamic over scales of both space and time. In order to capture adsorption onto and transport within these mixtures, we conduct simultaneous second harmonic generation (SHG) and two photon fluorescence measurements on two different gram-positive bacterial species as the cells uptake membrane-specific probe molecules. Our results show that SHG can not only monitor the movement of small molecules across membrane leaflets, bu...
Published on Sep 1, 2019in Biophysical Journal3.67
Fengfei Wang , Li-Zhen Sun + 2 AuthorsXiaojun Xu11
Estimated H-index: 11
Abstract RNA functions are often kinetically controlled. The folding kinetics of RNAs involves global structural changes and local nucleotide movement, such as base flipping. The most elementary step in RNA folding is the closing and opening of a base pair. By integrating molecular dynamics simulation, master equation, and kinetic Monte Carlo simulation, we investigate the kinetics mechanism of RNA helix-terminal base pairing. The study reveals a six-state folding scheme with three dominant fold...
Published on Sep 1, 2019in Biophysical Journal3.67
Michael A. Kelley (Colorado School of Mines), Karin Leiderman11
Estimated H-index: 11
(Colorado School of Mines)
Abstract Thrombin plays many important roles in the blood clotting process; it activates platelets, cleaves coagulation proteins within feedback loops, and cleaves fibrinogen into fibrin, which polymerizes into fibers to form a stabilizing gel matrix in and around growing clots. Thrombin also binds to the formed fibrin matrix but this interaction is not well understood. Thrombin-fibrin binding is typically described as two independent, single-step binding events, one high- and one low-affinity. ...
Published on 2019in Biophysical Journal3.67
Martin Oheim26
Estimated H-index: 26
(University of Paris),
Adi Salomon17
Estimated H-index: 17
(BIU: Bar-Ilan University)
+ 2 AuthorsUte Becherer17
Estimated H-index: 17
(Saarland University)
Abstract Roughly half of a cell’s proteins are located at or near the plasma membrane. In this restricted space, the cell senses its environment, signals to its neighbors and ex-changes cargo through exo- and endocytotic mechanisms. Ligands bind to receptors, ions flow across channel pores, and transmitters and metabolites are transported against con-centration gradients. Receptors, ion channels, pumps and transporters are the molecular substrates of these biological processes and they constitut...
Published on Aug 1, 2019in Biophysical Journal3.67
Thais A. Enoki2
Estimated H-index: 2
(Cornell University),
Gerald W. Feigenson40
Estimated H-index: 40
(Cornell University)
Abstract We describe a new method to prepare asymmetric giant unilamellar vesicles (aGUVs) via hemifusion. Hemifusion of giant unilamellar vesicles and a supported lipid bilayer, triggered by calcium, promotes the lipid exchange of the fused outer leaflets mediated by lipid diffusion. We used different fluorescent dyes to monitor the inner and the outer leaflets of the unsupported aGUVs. We confirmed that almost all newly exchanged lipids in the aGUVs are found in the outer leaflet of these asym...