An in vitro study of the effect of the optimal irrigation solution conditions during canine articular surgery

To establish whether chondrocyte viability, matrix degradation and the induction of proteolytic gene expression in canine cartilage is independent of irrigation fluid osmolality and time following exposure to the irrigation fluid. Canine cartilage explants were exposed to one of three different solution types i) Culture medium (270–280 mOsmol/kg) ii) NaCl 0.9% (302 mOsmol/kg) iii) NaCl 0.9% with sucrose (600 mOsmol/kg). Chondrocyte viability and...