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Veterinary Research Communications
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Post-mortem surveillance in Ireland discloses skin-test negative cattle with presumptive evidence of infection of Mycobacterium bovis (lesions at routine slaughter (LRS)), the causative agent of bovine tuberculosis (bTB). Laboratory confirmation of lesions has impacts on trade restrictions for herds, therefore if laboratory capacity was diminished, how herds are treated would require an informed risk policy. Here we report the proportion of herds with subsequent evidence of within-herd transmiss...
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#1Mizuki Ogawa (Nippon Veterinary and Life Science University)
#2Mio Ishizaka (Nippon Veterinary and Life Science University)
Last. Naoyuki Takemura (Nippon Veterinary and Life Science University)H-Index: 11
view all 8 authors...
BACKGROUND Sildenafil improves autonomic dysfunction caused by pulmonary hypertension (PH) in humans, but its effect is unknown in dogs with PH. This prospective study aimed to evaluate the autonomic nervous system function of a canine model of chronic embolic PH (CEPH) and the autonomic nervous system function of a canine model of CEPH in which sildenafil was administered. METHODS This study used five clinically healthy female beagle dogs. Evaluation parameters included hemodynamic parameters, ...
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#1Zhengyi Lian (Tianjin Agricultural University)H-Index: 1
#2Jie Bai (Tianjin Agricultural University)
Last. Yajiao Song (Tianjin Agricultural University)H-Index: 1
view all 7 authors...
Aeromonas salmonicida is one of the most important pathogens in salmonids and non-salmonids species. Nevertheless, very little was reported in cyprinids about A. salmonicida infection. Hence, a pathogenic A. salmonicida subsp. salmonicida, namely isolate GCA-518, was isolated from diseased crucian carp Carassius auratus. Its optimal growth conditions were at 28 °C, pH 7.0 and 1.5% NaCl. Furthermore, the quantitative real-time PCR (qPCR) targeting serine protease (aspA) gene was established for r...
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#1Malena Boylan (Teagasc)
#2Megan B. O’Brien (Teagasc)
Last. Kieran G. Meade (Teagasc)H-Index: 22
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OBJECTIVES: The faecal-oral route is a predominant mode of infectious disease transmission and yet the immunology of the bovine oral cavity is poorly understood. The objectives of this study were to develop an in vitro cell model of bovine salivary gland cells and to characterize the role of vitamin D on the expression of innate immune genes induced by stimulation with bacterial and viral pathogen-associated molecular patterns (PAMPs). METHODS: Submandibular glandular tissue was excised post-mor...
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#1Huijeong Ahn (Kangwon National University)H-Index: 10
#2Jeongeun Kim (Kangwon National University)H-Index: 3
Last. Geun-Shik Lee (Kangwon National University)H-Index: 23
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Inflammasome, a cytosolic multi-protein complex, assembly is a response to sensing intracellular pathogenic and endogenic danger signals followed by caspase-1 activation, which maturates precursor cytokines such as interleukin (IL)-1beta. Most inflammasome research has been undertaken in humans and rodents, and inflammasomes in veterinary species have not been well-characterized. In this study, we observed the effects of well-known inflammasome activators on equine peripheral blood monocytes (PB...
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#1Luiza Lopes da Silva (UFPEL: Universidade Federal de Pelotas)
#2Maiele Dornelles Silveira (ULBRA: Universidade Luterana do Brasil)H-Index: 2
Last. Nance Beyer Nardi (ULBRA: Universidade Luterana do Brasil)H-Index: 24
view all 6 authors...
Mesenchymal stromal cells (MSCs) have attracted great attention for therapeutic applications. Since cells derived from different tissues have different properties, using the right tissue source may impact their efficiency in regenerative medicine. This study describes for the first time the isolation and characterization of MSCs derived from the equine coronary corium, which may be useful for treating diseases such as laminitis. Seven coronary corium samples were used for isolation of cells (ccM...
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#1Paul Kelly (Teagasc)H-Index: 2
#2Anne Barry-Reidy (Teagasc)H-Index: 1
Last. Cliona O' Farrelly (Trinity College, Dublin)H-Index: 54
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Isolation and culture of distinct primary endometrial cells are key to reliable in-vitro models to investigate the uterine immune response and optimse new disease interventions. Details on the isolation method and purity of distinct cell populations is lacking in currently available protocols leading to inconsistent results across laboratories. Bovine endometrial tissue from non-pregnant bovine uteri were collected immediately post-mortem and separated using differential size filtering. Isolatio...
1 CitationsSource
Sub-acute ruminal acidosis is a type of metabolic disorder in which affected cattle show a considerable depression of rumen pH. This leads to a dramatic decline in productivity and consequent loss of income for many dairy farms. The objective of the present study is to identify and characterize novel long non-coding RNAs (lncRNAs) in Holstein cattle affected by sub-acute ruminal acidosis. Two replicates from six animals were sequenced that bioinformatically analyzed. Results showed 6679 novel ln...
1 CitationsSource
#2Dylan N. Clements (Edin.: University of Edinburgh)H-Index: 19
Last. Craig Johnson (UoB: University of Bristol)H-Index: 2
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Objectives To establish whether chondrocyte viability, matrix degradation and the induction of proteolytic gene expression in canine cartilage is independent of irrigation fluid osmolality and time following exposure to the irrigation fluid.
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Abundance and antibiotic resistance of bacteria of the genus Aeromonas isolated from the water of three carp ponds were studied. The number of those bacteria differed between the studied ponds, sites and season. The results of the present study showed that planktonic Aeromonas inhabiting those ponds strongly differed in the resistance level to tested antibiotics. These microorganisms were the most resistant to amoxicillin, ampicillin, clindamycin and penicillin. However, all isolates Aeromonas w...
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