Characterization and development of SSR markers of Pinctada maxima by RNA-Seq approach

Volume: 15, Pages: 100230 - 100230
Published: Nov 1, 2019
Abstract
In this study, we developed microsatellite markers, analyzed their distribution and frequency and verified polymorphism based on the transcriptome data of pearl oyster Pinctada maxima. Totally, 58,121 potential EST-SSRs from 145,877 sequences were identified. We randomly selected 100 of these primer pairs for further validation in the stock, 76 primer pairs were successfully amplified and 25 primer pairs were found to be polymorphic. The number...
Paper Details
Title
Characterization and development of SSR markers of Pinctada maxima by RNA-Seq approach
Published Date
Nov 1, 2019
Volume
15
Pages
100230 - 100230
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