Impact of sequencing depth and technology on de novo RNA-Seq assembly
Abstract
RNA-Seq data is inherently nonuniform for different transcripts because of differences in gene expression. This makes it challenging to decide how much data should be generated from each sample. How much should one spend to recover the less expressed transcripts? The sequencing technology used is another consideration, as there are inevitably always biases against certain sequences. To investigate these effects, we first looked at high-depth...
Paper Details
Title
Impact of sequencing depth and technology on de novo RNA-Seq assembly
Published Date
Jul 23, 2019
Journal
Volume
20
Issue
1
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