Quartz-Seq2: a high-throughput single-cell RNA-sequencing method that effectively uses limited sequence reads
Abstract
High-throughput single-cell RNA-seq methods assign limited unique molecular identifier (UMI) counts as gene expression values to single cells from shallow sequence reads and detect limited gene counts. We thus developed a high-throughput single-cell RNA-seq method, Quartz-Seq2, to overcome these issues. Our improvements in the reaction steps make it possible to effectively convert initial reads to UMI counts, at a rate of 30–50%, and detect more...
Paper Details
Title
Quartz-Seq2: a high-throughput single-cell RNA-sequencing method that effectively uses limited sequence reads
Published Date
Mar 9, 2018
Journal
Volume
19
Issue
1
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