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Enhancement of Lysozyme Crystallization Using DNA as a Polymeric Additive

Published on Apr 1, 2019
· DOI :10.3390/cryst9040186
Bo Zhang1
Estimated H-index: 1
,
Yao Wang + 6 AuthorsJerry Y. Y. Heng21
Estimated H-index: 21
Abstract
This study reports the first experimental evidence of using DNA as a polymeric additive to enhance protein crystallization. Using three kinds of DNA with different molecular weights—calf DNA, salmon DNA, and herring DNA—this study showed an improvement in the success rate of lysozyme crystallization, as compared to control experiments, especially at low lysozyme concentration. The improvement of crystallization is particularly significant in the presence of calf DNA with the highest molecular weight. Calf DNA also speeds up the induction time of lysozyme crystallization and increases the number of crystals per drop. We hypothesized the effect of DNA on protein crystallization may be due to the combination of excluded volume effect, change of water’s surface tension, and the water competition effect. This work confirms predications of the potential use of DNA as a polymeric additive to enhance protein crystallization, potentially applied to systems with limited protein available or difficult to crystallize.
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References41
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#1Bo Zhang (RUC: Renmin University of China)H-Index: 1
#2Andy Ran Mei (Imperial College London)H-Index: 1
Last.Jerry Y. Y. Heng (Imperial College London)H-Index: 21
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#1Xiaoguo Liu (CAS: Chinese Academy of Sciences)H-Index: 11
#2Fei Zhang (ASU: Arizona State University)H-Index: 13
Last.Lihua Wang (CAS: Chinese Academy of Sciences)H-Index: 35
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#1Xinmeng Tong (TJU: Tianjin University)H-Index: 1
#2Junjie Kang (Shihezi University)H-Index: 2
Last.Wei Li (TJU: Tianjin University)H-Index: 28
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#1Ren-Bin Zhou (NPU: Northwestern Polytechnical University)H-Index: 5
#2Hui-Ling CaoH-Index: 6
Last.Da-Chuan Yin (NPU: Northwestern Polytechnical University)H-Index: 16
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#1Umang V. Shah (Imperial College London)H-Index: 10
#2Niklas H. Jahn (Imperial College London)H-Index: 1
Last.Jerry Y. Y. Heng (Imperial College London)H-Index: 21
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#1Yong-Ming Liu (NPU: Northwestern Polytechnical University)H-Index: 7
#2Hai-Sheng Li (NPU: Northwestern Polytechnical University)H-Index: 5
Last.Da-Chuan Yin (NPU: Northwestern Polytechnical University)H-Index: 16
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#1Umang V. Shah (Imperial College London)H-Index: 10
#2Christian Amberg (Syngenta)H-Index: 1
Last.Jerry Y. Y. Heng (Imperial College London)H-Index: 21
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