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Jerry Y. Y. Heng
Imperial College London
104Publications
22H-index
1,767Citations
Publications 106
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As a purification technology, crystallisation is advantageous over chromatography and precipitation in terms of purity, cost and scalability. In general, proteins have slow crystallisation kinetics due to their complex configurations, but this problem can be overcome with heterogeneous nucleants. High protein-loading mesoporous silica is a promising nucleant due to its favourable interaction with protein. The current study employs such a template in the batch crystallisation of lysozyme and thau...
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With the increase in applications of protein-based medicines approved and developed, downstream manufacturing of biopharmaceuticals has challenges of finding cost-effective and reliable routes. Biocrystallization and centrifugation have both been used to isolate and purify macromolecules, such as therapeutic protein and other functional proteins. However, the centrifugation cannot perfectly separate biomolecules, and the biocrystallization mainly focuses on structure determination on a scale of ...
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#1Jinbo Ouyang (CUTe: China University of Technology)H-Index: 7
#2Limin Zhou (CUTe: China University of Technology)H-Index: 4
Last.Jerry Y. Y. Heng (Imperial College London)H-Index: 22
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Abstract In this paper, several common solvents including butyl acetate, methyl acetate, ethyl acetate, acetonitrile, 1-butanol, isobutanol, isopropanol, ethanol, 1-propanol, methanol, acetone, and water, were selected as solubility solvents for benzamide. The solubility of benzamide in all solvents increases with the increase of temperature from 283.15 K to 323.15 K, and generally depends on solvents in the following order: methanol > acetone > ethanol > 1-propanol > 1-butanol > isopropanol > i...
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#1Tomer Lapidot (Imperial College London)
#2Omar Matar (Imperial College London)H-Index: 30
Last.Jerry Y. Y. Heng (Imperial College London)H-Index: 22
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Abstract A population balance model is used to investigate the effect of mesoporous silica particles on calcium sulphate crystallisation in a stirred batch crystalliser. The model accounts for nucleation, growth, agglomeration, breakage, and particle-assisted nucleation, and the model equations are solved numerically using the method of classes over a logarithmic, non-uniform mesh. The crystallisation process is characterized experimentally using electrical conductivity to track the ion concentr...
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#1Martin Morgeneyer (University of Paris)H-Index: 11
#2Álvaro Ramírez Gómez (UPM: Technical University of Madrid)H-Index: 16
Last.Christophe BressotH-Index: 10
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Abstract "Can particle technology, in spite of its multiplicity, be regarded, and scientifically taught, as a uniform discipline?" wondered H. Rumpf, one of the founding fathers of particle science, in a programmatic speech which he gave 40 years ago. Given his passion for particle science, he of course answered his question with "yes". Now, 40 years later, working parties of the European Federation of Chemical Engineering, all working on particle technology, managed to organize their second joi...
7 CitationsSource
#1Jiayun Zou (JLU: Jilin University)
#2Juntao Wu (Ministry of Education)
Last.Jerry Y. Y. Heng (Imperial College London)H-Index: 22
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This study tests the effect of using the combination of graphene oxide (GO) with different valence cations as a heterogeneous nucleant on promoting catalase crystallization. By using GO and three types of salts with different valences, NaCl, MgCl2, and YCl3, the addition of GO with all three salts resulted in an increase in the percentage of crystal drops and a decrease in induction time. The experimental results further verified that there is a synergistic effect of GO and cations as the percen...
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#1Huaiyu YangH-Index: 8
Last.Jerry Y. Y. HengH-Index: 22
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The crystal form is one of the preferred formulations for biotherapeutics, especially thanks to its ability to ensure high stability of the active ingredient. In addition, crystallization allows the recovery of a very pure drug, thus facilitating the manufacturing process. However, in many cases, crystallization is not trivial, and other formulations, such as the concentrate solution, represent the only choice. This is the case of anti-cluster of differentiation 20 (anti-CD20), which is one of t...
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#1Bo ZhangH-Index: 1
Last.Jerry Y. Y. HengH-Index: 22
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This study reports the first experimental evidence of using DNA as a polymeric additive to enhance protein crystallization. Using three kinds of DNA with different molecular weights—calf DNA, salmon DNA, and herring DNA—this study showed an improvement in the success rate of lysozyme crystallization, as compared to control experiments, especially at low lysozyme concentration. The improvement of crystallization is particularly significant in the presence of calf DNA with the highest molecular we...
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#1Yen Wan Ngeow (Malaysian Rubber Board)H-Index: 1
#2Andrew V. Chapman (Tun Abdul Razak Research Centre)H-Index: 3
Last.Colin D. Hull (Tun Abdul Razak Research Centre)H-Index: 2
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ABSTRACT Tire treads with reduced rolling resistance and increased wet grip can be achieved by coupling hydrophilic silica to hydrocarbon rubber by using an alkoxysilane. The silica surface was mod...
2 CitationsSource
#1Y. W. Ngeow (Malaysian Rubber Board)H-Index: 1
#2Jerry Y. Y. Heng (Imperial College London)H-Index: 22
Last.Andrew V. Chapman (Tun Abdul Razak Research Centre)H-Index: 3
view all 6 authors...
The microdispersion of silica in filled elastomer vulcanisates was evaluated by transmission electron microscopy (TEM)—network visualisation analysis. In this study, the silica is modified with a wide range of coupling and non-coupling silanes. The silica-filled elastomer samples were microtomed using a glass knife. The resulting TEM micrographs were interpreted and characterised based on aggregates with a cross-sectional area larger than 100 nm2. The study showed that silica surface modificatio...
1 CitationsSource
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