Yao Wang
Ministry of Education
Protein crystallizationValence (chemistry)ChemistryCrystallizationLysozyme
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Publications 2
#1Bo ZhangH-Index: 2
#2Yao WangH-Index: 1
Last. Jerry Y. Y. HengH-Index: 23
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This study reports the first experimental evidence of using DNA as a polymeric additive to enhance protein crystallization. Using three kinds of DNA with different molecular weights—calf DNA, salmon DNA, and herring DNA—this study showed an improvement in the success rate of lysozyme crystallization, as compared to control experiments, especially at low lysozyme concentration. The improvement of crystallization is particularly significant in the presence of calf DNA with the highest molecular we...
2 CitationsSource
#1Jiayun Zou (JLU: Jilin University)H-Index: 1
#2Juntao Wu (Ministry of Education)H-Index: 1
Last. Jerry Y. Y. Heng (Imperial College London)H-Index: 23
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This study tests the effect of using the combination of graphene oxide (GO) with different valence cations as a heterogeneous nucleant on promoting catalase crystallization. By using GO and three types of salts with different valences, NaCl, MgCl2, and YCl3, the addition of GO with all three salts resulted in an increase in the percentage of crystal drops and a decrease in induction time. The experimental results further verified that there is a synergistic effect of GO and cations as the percen...
1 CitationsSource