Transcriptomic response of primary human airway epithelial cells to flavoring chemicals in electronic cigarettes.

Published on Feb 1, 2019in Scientific Reports4.01
· DOI :10.1038/s41598-018-37913-9
Hongkun Park49
Estimated H-index: 49
(Harvard University),
Michael J. O'Sullivan1
Estimated H-index: 1
(Harvard University)
+ 6 AuthorsQuan Lu19
Estimated H-index: 19
(Harvard University)
The widespread use of electronic cigarettes (e-cigarettes or e-cig) is a growing public health concern. Diacetyl and its chemical cousin 2,3-pentanedione are commonly used to add flavors to e-cig; however, little is known about how the flavoring chemicals may impair lung function. Here we report that the flavoring chemicals induce transcriptomic changes and perturb cilia function in the airway epithelium. Using RNA-Seq, we identified a total of 163 and 568 differentially expressed genes in primary normal human bronchial epithelial (NHBE) cells that were exposed to diacetyl and 2,3-pentanedione, respectively. DAVID pathway analysis revealed an enrichment of cellular pathways involved in cytoskeletal and cilia processes among the set of common genes (142 genes) perturbed by both diacetyl and 2,3-pentanedione. Consistent with this, qRT-PCR confirmed that the expression of multiple genes involved in cilia biogenesis was significantly downregulated by diacetyl and 2,3-pentanedione in NHBE cells. Furthermore, immunofluorescence staining showed that the number of ciliated cells was significantly decreased by the flavoring chemicals. Our study indicates that the two widely used e-cig flavoring chemicals impair the cilia function in airway epithelium and likely contribute to the adverse effects of e-cig in the lung.
  • References (76)
  • Citations (2)
#1Eric J. Zaccone (WVU: West Virginia University)H-Index: 3
#2W. Travis Goldsmith (NIOSH: National Institute for Occupational Safety and Health)H-Index: 7
Last.Jeffrey S. Fedan (WVU: West Virginia University)H-Index: 26
view all 9 authors...
#1Xuefei Cao (FDA: Food and Drug Administration)H-Index: 6
#2Haixia Lin (FDA: Food and Drug Administration)H-Index: 1
Last.Robert H. HeflichH-Index: 41
view all 6 authors...
#1Robert McMillen (American Academy of Pediatrics)H-Index: 17
#2Mark A. Gottlieb (NU: Northeastern University)H-Index: 10
Last.Jonathan D. Klein (American Academy of Pediatrics)H-Index: 54
view all 5 authors...
#1Monique Williams (UCR: University of California, Riverside)H-Index: 8
#2An To (UCR: University of California, Riverside)H-Index: 1
Last.Prue Talbot (UCR: University of California, Riverside)H-Index: 28
view all 4 authors...
Cited By2
#1Grzegorz Brożek (University of Silesia in Katowice)H-Index: 4
#2Mateusz Jankowski (University of Silesia in Katowice)H-Index: 2
Last.Jan E. Zejda (University of Silesia in Katowice)H-Index: 11
view all 3 authors...
#1Jeffrey E. Gotts (UCSF: University of California, San Francisco)H-Index: 13
#2Sven-Eric Jordt (Yale University)H-Index: 27
Last.Robert Tarran (UNC: University of North Carolina at Chapel Hill)H-Index: 31
view all 0 authors...
View next paperInflammatory Response and Barrier Dysfunction by Different e-Cigarette Flavoring Chemicals Identified by Gas Chromatography–Mass Spectrometry in e-Liquids and e-Vapors on Human Lung Epithelial Cells and Fibroblasts