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Potential reservoirs and routes of Cronobacter transmission during cereal growing, processing and consumption

Published on Jun 1, 2019in Food Microbiology 4.09
· DOI :10.1016/j.fm.2018.12.004
Xiuqin Lou2
Estimated H-index: 2
(CDC: Centers for Disease Control and Prevention),
Hua Yu2
Estimated H-index: 2
(CDC: Centers for Disease Control and Prevention)
+ 9 AuthorsZhiguo Fang2
Estimated H-index: 2
(ZJSU: Zhejiang Gongshang University)
Cite
Abstract
Abstract Cronobacter are opportunistic bacterial pathogens of both infants and adults. We investigated the incidence and distribution of Cronobacter in 1245 samples of cereal and related environments. 39.1% (101/258) rice-related and 46.9% (98/209) wheat-related samples tested positive for Cronobacter , and the positive rate differed notably according to processing method. Cronobacter was found in rice and wheat plants at the tillering, filling and mature stages. Soil, water and swab samples from nearby milling plants were assayed, and results revealed that 6.3% (7/122) of water from paddy fields, 49.1% (28/57) and 62.1% (41/67) of swab samples from rice and wheat flour milling plants were Cronobacter positive. Pulsed-field gel electrophoresis (PFGE) subtyping indicated that some strains had a common profile, which suggested their persistence in the environment, potential transmission routes and cross-contamination in processing. Finally, we surveyed 18 families to evaluate potential risks. None of the families who primarily ate rice cooked with water tested positive for Cronobacter , though of 66.7% families (6/9) whose food staples were produced from wheat flour tested positive. Taken together, our results are important for understanding Cronobacter transmission and will aid in the development of additional control measures to reduce the risk of infection by these opportunistic pathogenic bacteria.
  • References (32)
  • Citations (1)
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References32
Newest
Published on May 1, 2018in Food Research International 3.58
Luiza Vasconcellos2
Estimated H-index: 2
(FIOCRUZ: Oswaldo Cruz Foundation),
Carla Trece Carvalho2
Estimated H-index: 2
+ 6 AuthorsMarcelo Luiz Lima Brandão5
Estimated H-index: 5
(FIOCRUZ: Oswaldo Cruz Foundation)
Abstract The aim of this study was to detect Cronobacter from 30 samples of ready-to-eat (RTE) salads and 30 foods from Japanese cuisine as commercially available in Brazil. The detection of Cronobacter was as according to the ISO standard 22964:2017. The isolates were phenotypically characterized by Vitek 2.0 and the antibiotic susceptibility profile was determined using the standardized agar disc diffusion method. Molecular characterization was accomplished by real-time PCR targeting dnaG gene...
Published on Aug 1, 2017in Food Microbiology 4.09
Anna Berthold-Pluta4
Estimated H-index: 4
(Warsaw University of Life Sciences),
Monika Garbowska4
Estimated H-index: 4
+ 1 AuthorsAntoni Pluta3
Estimated H-index: 3
(Warsaw University of Life Sciences)
Bacteria of the genus Cronobacter are emerging food-borne pathogens. Foods contaminated with Cronobacter spp. may pose a risk to infants or adults with suppressed immunity. This study was aimed at determining the microbiological quality of ready-to-eat (RTE) plant-origin food products available on the Polish market with special emphasis on the prevalence of Cronobacter genus bacteria. Analyses were carried out on 60 samples of commercial RTE type plant-origin food products, including: leaf veget...
Published on May 1, 2017in Food Microbiology 4.09
Marcelo Luiz Lima Brandão5
Estimated H-index: 5
(FIOCRUZ: Oswaldo Cruz Foundation),
Natália Scudeller Umeda3
Estimated H-index: 3
(FIOCRUZ: Oswaldo Cruz Foundation)
+ 2 AuthorsIvano de Filippis9
Estimated H-index: 9
(FIOCRUZ: Oswaldo Cruz Foundation)
Abstract Several Cronobacter species are opportunistic pathogens that cause infections in humans. The aim of this study was to detect Cronobacter spp. from 90 samples of retail foods in Brazil, and characterize the strains by phenotypic tests, molecular assays and antibiotic susceptibility. Three isolation methodologies were evaluated using different selective enrichments and the isolates were identified using Vitek 2.0, PCRs protocols, fusA allele sequencing and multilocus sequence typing (MLST...
Published on Jan 1, 2017in Biocontrol Science 0.81
Shigeko Ueda9
Estimated H-index: 9
(Kagawa Nutrition University)
Published on Jun 1, 2016in World Journal of Microbiology & Biotechnology 2.65
Wanyi Chen1
Estimated H-index: 1
,
Jielin Yang1
Estimated H-index: 1
+ 1 AuthorsZhenmin Liu1
Estimated H-index: 1
Cronobacter spp. has caused life-threatening neonatal infections mainly resulted from consumption of contaminated powdered infant formula. A total of 102 vegetable samples from retail markets were evaluated for the presence of Cronobacter spp. Thirty-five presumptive Cronobacter isolates were isolated and identified using API 20E and 16S rDNA sequencing analyses. All isolates and type strains were characterized using enterobacterial repetitive intergenic consensus sequence PCR (ERIC–PCR), and ge...
Published on Feb 1, 2016in Biomedical and Environmental Sciences 1.92
Pei Xy1
Estimated H-index: 1
(CFS: Center for Food Safety),
Yan L1
Estimated H-index: 1
(CFS: Center for Food Safety)
+ 7 AuthorsYang da J1
Estimated H-index: 1
(CFS: Center for Food Safety)
Abstract Objective To determine Cronobacter spp. contamination in infant and follow-up powdered formula in China. Methods All of 2282 samples were collected from the retail markets in China from January 2012 to December 2012, and analyzed for Cronobacter spp . by the Chinese National Food Safety Standard . Characterization of the isolates was analyzed by pulsed-field gel electrophoresis (PFGE) with Xba I and Spe I restriction enzymes. Results Cronobacter spp. strains were isolated from 25 sample...
Published on Jan 1, 2016in International Journal of Food Microbiology 4.01
Vojkovská H2
Estimated H-index: 2
,
Renata Karpíšková16
Estimated H-index: 16
+ 1 AuthorsHana Drahovská15
Estimated H-index: 15
(Comenius University in Bratislava)
Abstract The Cronobacter genus (previously known as Enterobacter sakazakii ) comprises seven species ( Cronobacter sakazakii , Cronobacter malonaticus , Cronobacter muytjensii , Cronobacter turicensis , Cronobacter dublinensis , Cronobacter universalis and Cronobacter condimenti ) which cause serious infections in neonates and immunocompromised people. Most of the documented outbreaks of these bacteria have been associated with consumption of contaminated powdered infant formula. The plant envir...
Published on Dec 1, 2015in SpringerPlus
Norrakiah Abdullah Sani8
Estimated H-index: 8
(UKM: National University of Malaysia),
Olumide A. Odeyemi6
Estimated H-index: 6
(UTAS: University of Tasmania)
Cronobacter species are motile, non-spore forming, Gram negative emerging opportunistic pathogens mostly associated with bacteremia, meningitis, septicemia, brain abscesses and necrotizing enterocolitis in infected neonates, infants and immunocompromised adults. Members of the genus Cronobacter are previously associated with powdered infant formula although the main reservoir and routes of contamination are yet to be ascertained. This study therefore aim to summarize occurrence and prevalence of...
Abdlrhman Alsonosi2
Estimated H-index: 2
(NTU: Nottingham Trent University),
Sumyya Hariri8
Estimated H-index: 8
(NTU: Nottingham Trent University)
+ 8 AuthorsStephen J. Forsythe44
Estimated H-index: 44
(NTU: Nottingham Trent University)
The World Health Organization (WHO) has recognised all Cronobacter species as human pathogens. Among premature neonates and immunocompromised infants, these infections can be life-threatening, with clinical presentations of septicaemia, meningitis and necrotising enterocolitis. The neurological sequelae can be permanent and the mortality rate as high as 40–80 %. Despite the highlighted issues of neonatal infections, the majority of Cronobacter infections are in the elderly population suffering f...
Cited By1
Newest
Published on Jul 1, 2019in Food Microbiology 4.09
Xiuqin Lou2
Estimated H-index: 2
(CDC: Centers for Disease Control and Prevention),
Tao Liu2
Estimated H-index: 2
(CDC: Centers for Disease Control and Prevention)
+ 5 AuthorsZhiguo Fang2
Estimated H-index: 2
(ZJSU: Zhejiang Gongshang University)
Abstract Cronobacter was positive in cereals at a relatively high rate. In the present study, we investigated the occurrence and characteristics of this pathogen systematically in diverse cereals. All sampled food (N = 467) contained Cronobacter with a high positive rate of 54.0%. The enumeration experiment showed the concentration ranged from 0.3 to more than 110 MPN/100 g, and 87.9% of 127 samples were less than 10 MPN/100 g. There were significant differences ( p Cronobacter among cereal kern...