Match!

Mountain pine beetle (Dendroctonus ponderosae) CYP4Gs convert long and short chain alcohols and aldehydes to hydrocarbons

Published on Nov 1, 2018in Insect Biochemistry and Molecular Biology3.62
· DOI :10.1016/j.ibmb.2018.09.005
Marina MacLean3
Estimated H-index: 3
(UNR: University of Nevada, Reno),
Jeffrey Nadeau3
Estimated H-index: 3
(UNR: University of Nevada, Reno)
+ 2 AuthorsGary J. Blomquist49
Estimated H-index: 49
(UNR: University of Nevada, Reno)
Cite
Abstract
Abstract Hydrocarbon biosynthesis in insects involves the elongation of fatty acyl-CoAs to very-long chain fatty acyl-CoAs that are then reduced and converted to hydrocarbon, with the last step involving the oxidative decarbonylation of an aldehyde to hydrocarbon and carbon dioxide. Cytochromes P450 in the 4G family decarbonylate aldehydes to hydrocarbon. All insect acyl-CoA reductases studied to date reduce fatty acyl-CoAs to alcohols. The results of the work reported herein demonstrate that CYP4G55 and CYP4G56 from the mountain pine beetle, Dendroctonus ponderosae , expressed as fusion proteins with house fly cytochrome P450 reductase (CPR), convert both long chain aldehydes and long chain alcohols to hydrocarbons. CYP4G55 and CYP4G56 appear to prefer primary alcohols to aldehydes as substrates. These data strongly suggest that hydrocarbon biosynthesis in insects occurs by the two-step reduction of very long chain fatty acyl-CoAs to alcohols, which are then oxidized to aldehydes and then oxidatively decarbonylated to hydrocarbon by CYP4G enzymes. In addition, both CYP4G55 and CYP4G56 fusion proteins convert C10 alcohols and aldehydes to hydrocarbons, including the conversion of ( Z )-7-decenal, a putative intermediate in the exo -brevicomin pheromone biosynthetic pathway, to ( Z )-3-nonene. These data demonstrate that the highly conserved CYP4G enzymes accept a broad range of carbon chain lengths, including C10 and C18, and have evolved to function in cuticular hydrocarbon biosynthesis and pheromone production.
  • References (44)
  • Citations (6)
Cite
References44
Newest
Published on Oct 1, 2018in Insect Molecular Biology2.44
Bernarda Calla11
Estimated H-index: 11
(UIUC: University of Illinois at Urbana–Champaign),
Marina MacLean3
Estimated H-index: 3
(UNR: University of Nevada, Reno)
+ 4 AuthorsMay R. Berenbaum67
Estimated H-index: 67
(UIUC: University of Illinois at Urbana–Champaign)
Published on Dec 1, 2017in BMC Genomics3.50
Jeffrey Nadeau3
Estimated H-index: 3
(UNR: University of Nevada, Reno),
Juli Petereit5
Estimated H-index: 5
(UNR: University of Nevada, Reno)
+ 7 AuthorsClaus Tittiger22
Estimated H-index: 22
(UNR: University of Nevada, Reno)
Abstract Background The mountain pine beetle (MPB, Dendroctonus ponderosae Hopkins) is a highly destructive pest of pine forests in western North America. During flight to a new host tree and initiation of feeding, mountain pine beetles release aggregation pheromones. The biosynthetic pathways of these pheromones are sex-specific and localized in the midgut and fat body, but the enzymes involved have not all been identified or characterized. Results We used a comparative RNA-Seq analysis between...
Published on Dec 1, 2016in Current Opinion in Chemical Biology8.54
Nicolaus A. Herman3
Estimated H-index: 3
(University of California, Berkeley),
Wenjun Zhang32
Estimated H-index: 32
(University of California, Berkeley)
Surging energy consumption and environmental concerns have stimulated interest in the production of chemicals and fuels through sustainable and renewable approaches. Fatty acid-based hydrocarbons, such as alkanes and alkenes, are of particular interest to directly replace fossil fuels. Towards this effort, understanding of hydrocarbon-producing enzymes is the first indispensable step to bio-production of hydrocarbons. Here, we review recent advances in the discovery and mechanistic study of enzy...
Published on Sep 1, 2016in Insect Biochemistry and Molecular Biology3.62
Nan Chen3
Estimated H-index: 3
(NWAFU: Northwest A&F University),
Yongliang Fan15
Estimated H-index: 15
(NWAFU: Northwest A&F University)
+ 3 AuthorsTong-Xian Liu1
Estimated H-index: 1
(NWAFU: Northwest A&F University)
Terrestrial insects deposit a layer of hydrocarbons (HCs) as waterproofing agents on their epicuticle. The insect-specific CYP4G genes, subfamily members of P450, have been found in all insects with sequenced genomes to date. They are critical for HC biosynthesis in Drosophila; however, their functional roles in other insects including the piercing-sucking hemipterous aphids remain unknown. In this study, we presented the molecular characterization and a functional study of the CYP4G51 gene in t...
Vasileia Balabanidou4
Estimated H-index: 4
(FORTH: Foundation for Research & Technology – Hellas),
Anastasia Kampouraki4
Estimated H-index: 4
(UoC: University of Crete)
+ 12 AuthorsAmy Lynd11
Estimated H-index: 11
(LSTM: Liverpool School of Tropical Medicine)
The role of cuticle changes in insecticide resistance in the major malaria vector Anopheles gambiae was assessed. The rate of internalization of (14)C deltamethrin was significantly slower in a resistant strain than in a susceptible strain. Topical application of an acetone insecticide formulation to circumvent lipid-based uptake barriers decreased the resistance ratio by ∼50%. Cuticle analysis by electron microscopy and characterization of lipid extracts indicated that resistant mosquitoes had ...
Published on Aug 8, 2016in PLOS Genetics5.22
Einat Cinnamon2
Estimated H-index: 2
(Francis Crick Institute),
Rami Makki2
Estimated H-index: 2
(Francis Crick Institute)
+ 5 AuthorsAlex P. Gould5
Estimated H-index: 5
(Francis Crick Institute)
Cell growth and proliferation depend upon many different aspects of lipid metabolism. One key signaling pathway that is utilized in many different anabolic contexts involves Phosphatidylinositide 3-kinase (PI3K) and its membrane lipid products, the Phosphatidylinositol (3,4,5)-trisphosphates. It remains unclear, however, which other branches of lipid metabolism interact with the PI3K signaling pathway. Here, we focus on specialized fat metabolizing cells in Drosophila called larval oenocytes. In...
Published on Jul 1, 2016in Scientific Reports4.01
Zhitao Yu4
Estimated H-index: 4
,
Xueyao Zhang8
Estimated H-index: 8
+ 5 AuthorsJianzhen Zhang21
Estimated H-index: 21
Citation: Yu, Z. T., Zhang, X. Y., Wang, Y. W., Moussian, B., Zhu, K. Y., Li, S., . . . Zhang, J. Z. (2016). LmCYP4G102: An oenocyte-specific cytochrome P450 gene required for cuticular waterproofing in the migratory locust, Locusta migratoria. Scientific Reports, 6, 11. doi:10.1038/srep29980
Published on Jan 1, 2005
Russell A. Jurenka31
Estimated H-index: 31
(UNR: University of Nevada, Reno),
Gary J. Blomquist49
Estimated H-index: 49
(UNR: University of Nevada, Reno)
+ 1 AuthorsClaus Tittiger22
Estimated H-index: 22
The elucidation of the structure of the first insect sex pheromone, bombykol (( E , Z )-10,12-hexadecadien-1-ol) from the silkworm moth, Bombyx mori , spanned more than 20 years and required a half million female abdomens. A few years later, ( Z )-7-dodecenyl acetate was identified as the sex pheromone of the cabbage looper, Trichoplusia ni . At about the same time three terpenoid alcohols, ipsenol, ipsdienol, and verbenol were identified as the pheromone of the bark beetle, Ips paraconfusus . T...
Published on Jan 1, 2016
Matthew D. Ginzel4
Estimated H-index: 4
(Purdue University),
Gary J. Blomquist49
Estimated H-index: 49
(UNR: University of Nevada, Reno)
Cuticular hydrocarbons of insects often consist of complex mixtures of straight chain, unsaturated and methyl-branched components with 21 to 40+ carbons. They function to restrict water loss, to prevent a lethal rate of desiccation and serve in chemical communication in many species. This chapter describes the chemistry and chemical ecology of insect hydrocarbons with an emphasis on their role as close range or contact pheromones. Hydrocarbons are formed in oenocytes and their biosynthetic pathw...
Cited By6
Newest
Published on 2020in Current Opinion in Biotechnology8.08
Kun Liu (SDU: Shandong University), Shengying Li (SDU: Shandong University)
Enormous fuel consumption and growing environmental concerns have been spurring development of renewable biofuels. Among various biofuels, fatty acid-derived biohydrocarbons are ideal alternatives to non-renewable fossil fuels due to their closest properties to petroleum-based fuels. In the past decade, novel hydrocarbon-producing enzymes have continuously been discovered and engineered. Here, we review the recent advances in biosynthesis of fatty acid-derived hydrocarbons with emphasis on enzym...
Published on Feb 6, 2019in Scientific Reports4.01
Christine C. Chiu3
Estimated H-index: 3
(UBC: University of British Columbia),
Christopher I. Keeling26
Estimated H-index: 26
(UBC: University of British Columbia),
Joerg Bohlmann34
Estimated H-index: 34
(UBC: University of British Columbia)
The recent outbreak of the mountain pine beetle (Dendroctonus ponderosae; MPB) has affected over 20 M hectares of pine forests in western North America. During the colonization of host trees, female MPB release the aggregation pheromone (−)-trans-verbenol. (−)-trans-Verbenol is thought to be produced from the pine defense compound (−)-α-pinene by cytochrome P450 (P450) dependent hydroxylation. MPB may also use P450s for the detoxification of other monoterpenes of the pine defense system. Here we...
Published on 2019in Insect Biochemistry and Molecular Biology3.62
Xiao‐Jin Pei (NWAFU: Northwest A&F University), Nan Chen3
Estimated H-index: 3
(NWAFU: Northwest A&F University)
+ 4 AuthorsTong-Xian Liu34
Estimated H-index: 34
(NWAFU: Northwest A&F University)
Abstract Insect cuticular hydrocarbons (CHCs), the evolutionary products of aquatic hexapod ancestors expanding to terrestrial environment, are deposited on the surface of insect integument and originally functioned primarily as waterproofing agents. CHCs are derived from the conserved fatty acid synthesis pathway in insects. However, the pivotal fatty acid synthase (FAS) involved in hydrocarbon (HC) biosynthesis remains unknown in many insect orders including the primitive Blattodea. Here, we i...
Published on Aug 21, 2019in bioRxiv
Amy Lynd11
Estimated H-index: 11
(LSTM: Liverpool School of Tropical Medicine),
Vasileia Balabanidou4
Estimated H-index: 4
(FORTH: Foundation for Research & Technology – Hellas)
+ 4 AuthorsGareth Lycett14
Estimated H-index: 14
(LSTM: Liverpool School of Tropical Medicine)
Oenocytes are an insect cell type having diverse physiological functions ranging from cuticular hydrocarbon (CHC) production to insecticide detoxification that may impact their capacity to transmit pathogens. To develop functional genetic tools to study Anopheles gambiae oenocytes, we have trapped an oenocyte enhancer to create a transgenic mosquito Gal4 driver line that mediates tissue-specific expression. After crossing with UAS-reporter lines, An. gambiae oenocytes are fluorescently tagged th...
Published on Jul 1, 2019in Insect Biochemistry and Molecular Biology3.62
Mary Kefi1
Estimated H-index: 1
,
Vasileia Balabanidou4
Estimated H-index: 4
+ 3 AuthorsJohn Vontas39
Estimated H-index: 39
Abstract Cuticular hydrocarbon (CHC) biosynthesis is a major pathway of insect physiology. In Drosophila melanogaster the cytochrome P450 CYP4G1 catalyses the insect-specific oxidative decarbonylation step, while in the malaria vector Anopheles gambiae , two CYP4G paralogues, CYP4G16 and CYP4G17 are present. Analysis of the subcellular localization of CYP4G17 and CYP4G16 in larval and pupal stages revealed that CYP4G16 preserves its PM localization across developmental stages analyzed; however C...
Published on 2019in Genome Biology and Evolution3.73
Cédric Finet11
Estimated H-index: 11
(École normale supérieure de Lyon),
Kailey Slavik (Harvard University)+ 2 AuthorsHenry Chung13
Estimated H-index: 13
(MSU: Michigan State University)
Published on May 1, 2019in Insect Biochemistry and Molecular Biology3.62
Dan-Ting Li1
Estimated H-index: 1
(ZJU: Zhejiang University),
Xuan Chen3
Estimated H-index: 3
(ZJU: Zhejiang University)
+ 2 AuthorsChuan-Xi Zhang28
Estimated H-index: 28
(ZJU: Zhejiang University)
Abstract The cuticular hydrocarbon (CHC) biosynthetic pathways branches off from the synthesis of fatty acids. Fatty acid elongases (ELOs) are enzymes catalyzing the synthesis of long-chain fatty acids and thereby contribute to the diversification of CHCs. Based on bioinformatics analyses we identified 20 ELO genes in the brown planthopper, Nilaparvata lugens . RNA interference against these genes demonstrated that 9 NlELO genes were essential for the survival of N. lugens nymphs and adults. Ind...