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Insect Biochemistry and Molecular Biology
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3.62
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3534
Papers 3534
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#1Petra Sumova (Charles University in Prague)H-Index: 5
#2Michal Sima (Charles University in Prague)H-Index: 4
Last.Petr Volf (Charles University in Prague)H-Index: 42
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Abstract The amine-binding properties of sand fly salivary yellow-related proteins (YRPs) were described only in Lutzomyia longipalpis sand flies. Here, we experimentally confirmed the kratagonist function of YRPs in the genus Phlebotomus. We utilized microscale thermophoresis technique to determine the amine-binding properties of YRPs in saliva of Phlebotomus perniciosus and P. orientalis, the Old-World vectors of visceral leishmaniases causative agents. Expressed and purified YRPs from three d...
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#1Rose Waldron (MU: Maynooth University)H-Index: 2
#2Jamie McGowan (MU: Maynooth University)H-Index: 3
Last.Sean Doyle (MU: Maynooth University)H-Index: 31
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ABSTRACT The European house dust mite, Dermatophagoides pteronyssinus is a major source of airborne allergens worldwide and is found in half of European homes. Interactions between microbes and house dust mites (HDM) are considered important factors that allow them to persist in the home. Laboratory studies indicate the European HDM, D. pteronyssinus is a mycophagous mite, capable of utilising a variety of fungi for nutrients, however specific mycolytic digestive enzymes are unknown. Our previou...
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#1Justina Koubová (Sewanee: The University of the South)
#2Tomáš Jehlík (Sewanee: The University of the South)
Last.Radmila Čapková Frydrychová (Sewanee: The University of the South)H-Index: 9
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Abstract The attrition of telomeres, the ends of eukaryote chromosomes, and activity of telomerase, the enzyme that restores telomere length, play a role in the ageing process and act as indicators of biological age. A notable feature of advanced eusocial insects is the longevity of reproductive individuals (queens and kings) compared to those from non-reproductive castes (workers and soldiers) within a given species, with a proposed link towards upregulation of telomerase activity in the somati...
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#1Yu Zhao (SYSU: Sun Yat-sen University)
#2Gang Huang (SYSU: Sun Yat-sen University)
Last.Wenqing Zhang (SYSU: Sun Yat-sen University)H-Index: 13
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Abstract The brown planthopper (BPH) Nilaparvata lugens contains two insulin receptor homologues, designated NlInR1 and NlInR2. NlInR1 is strikingly homologous to the typical InR in insects and vertebrates, containing a ligand-activated intracellular tyrosine kinase catalytic domain. Herein, we report an optimized CRISPR/Cas9 system to induce mutations in the NlInR1 locus in BPH, consisting of a Cas9 plasmid that is specifically expressed in the germline via the Nlvasa promoter and versatile sgR...
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#1Mark Mallott (University of Exeter)
#2Sarah Hamm (University of Exeter)
Last.Chris Bass (University of Exeter)H-Index: 38
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Abstract The diamondback moth, Plutella xylostella, is a damaging pest of cruciferous crops, and has evolved resistance to many of the insecticides used for control, including members of the diamide class. Previous work on the molecular basis of resistance to diamides has documented mutations in the target-site, the ryanodine receptor, in resistant populations of P. xylostella worldwide. In contrast the role of metabolic resistance to this insecticide class is significantly less clear. Here we s...
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#1Guan-Heng Zhu (NAU: Nanjing Agricultural University)
#2Mei-Yan Zheng (NAU: Nanjing Agricultural University)H-Index: 2
Last.Shuang-Lin Dong (NAU: Nanjing Agricultural University)H-Index: 25
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Abstract Three different pheromone binding proteins (PBPs) can typically be found in the sensilla lymph of noctuid moth antennae, but their relative contributions in perception of the sex pheromone is rarely verified in vivo. Previously, we demonstrated that SlitPBP3 plays a minor role in the sex pheromone detection in Spodoptera litura using the CRISPR/Cas9 system. In the present study, the roles of two other SlitPBPs (SlitPBP1 and SlitPBP2) are further verified using the same system. First, by...
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#1Pingyang Wang (Jiangsu University)H-Index: 2
#2Qiaoling Zhao (Jiangsu University)H-Index: 6
Last.Xingjia Shen (Jiangsu University)H-Index: 6
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Abstract The natural colorful cuticles of insects play important roles in many physiological processes. Pigmentation is a physiological process with a complex regulatory network whose regulatory mechanism remains unclear. Bombyx mori pigmentation mutants are ideal materials for research on pigmentation mechanisms. The purple quail-like (q-lp) and brown quail-like (q-lb) mutants originated from plain silkworm breeds 932VR and 0223JH respectively exhibit similar cuticle pigmentation to that of the...
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#1Sooa Lim (Hoseo University)
#2Hwa-Kyung Yun (Hanseo University)H-Index: 3
Last.In Hee Lee (Hoseo University)H-Index: 14
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Abstract Mycoplasmas, the smallest self-replicating organisms, are unique in that they lack cell walls but possess distinctive plasma membranes containing sterol acquired from their growth environment. Although mycoplasmas are known to be successful pathogens in a wide range of animal hosts, including humans, the molecular basis for their virulence and interaction with the host immune systems remains largely unknown. This study was conducted to elucidate the biochemical relationship between myco...
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#1Alessandro Gaviraghi (UFRJ: Federal University of Rio de Janeiro)H-Index: 2
#2Juliana B. R. Correa Soares (UFRJ: Federal University of Rio de Janeiro)H-Index: 7
Last.Marcus F. Oliveira (UFRJ: Federal University of Rio de Janeiro)H-Index: 30
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Abstract The huge energy demand posed by insect flight activity is met by an efficient oxidative phosphorylation process that takes place within flight muscle mitochondria. In the major arbovirus vector Aedes aegypti, mitochondrial oxidation of pyruvate, proline and glycerol 3-phosphate (G3P) represent the major energy sources of ATP to sustain flight muscle energy demand. Although adenylates exert critical regulatory effects on several mitochondrial enzyme activities, the potential consequences...
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