Electrical Stimulation Prevents Preferential Skeletal Muscle Myosin Loss in Steroid-Denervation Rats

Published on Aug 10, 2018in Frontiers in Physiology3.20
· DOI :10.3389/fphys.2018.01111
Takashi Yamada23
Estimated H-index: 23
(RMIT: RMIT University),
Koichi Himori4
Estimated H-index: 4
(RMIT: RMIT University)
+ 10 AuthorsJohanna T. Lanner18
Estimated H-index: 18
(KI: Karolinska Institutet)
Severe muscle weakness concomitant with preferential depletion of myosin has been observed in several pathological conditions. Here we used the steroid-denervation (S-D) rat model, which shows dramatic decrease in myosin content and force production, to test whether electrical stimulation (ES) treatment can prevent these deleterious changes. S-D was induced by cutting the sciatic nerve and subsequent daily injection of dexamethasone for 7 days. For ES treatment, plantarflexor muscles were electrically stimulated to produce four sets of five isometric contractions each day. Plantarflexor in situ isometric torque, muscle weight, skinned muscle fiber force, and protein and mRNA expression were measured after the intervention period. ES treatment partly prevented the S-D-induced decreases in plantarflexor in situ isometric torque and muscle weight. ES treatment fully prevented S-D-induced decreases in skinned fiber force and ratio of myosin heavy chain (MyHC) to actin, as well as increases in the reactive oxygen/nitrogen species-generating enzymes NADPH oxidase (NOX) 2 and 4, phosphorylation of p38 MAPK, mRNA expression of the muscle-specific ubiquitin ligases muscle ring finger-1 (MuRF-1) and atrogin-1, and autolyzed active calpain-1. Thus, ES treatment is an effective way to prevent muscle impairments associated with loss of myosin.
  • References (47)
  • Citations (2)
#1Oliver Friedrich (FAU: University of Erlangen-Nuremberg)H-Index: 28
#2S. Diermeier (FAU: University of Erlangen-Nuremberg)H-Index: 1
Last.Lars Larsson (KI: Karolinska Institutet)H-Index: 46
view all 3 authors...
#1Koichi Himori (RMIT: RMIT University)H-Index: 4
#2Daisuke Tatebayashi (RMIT: RMIT University)H-Index: 4
Last.Takashi Yamada (RMIT: RMIT University)H-Index: 23
view all 7 authors...
#1Monica Llano-Diez (KI: Karolinska Institutet)H-Index: 2
#2Arthur J. Cheng (KI: Karolinska Institutet)H-Index: 15
Last.Joseph D. Bruton (KI: Karolinska Institutet)H-Index: 37
view all 9 authors...
#1Scott K. Powers (UF: University of Florida)H-Index: 74
#2Aaron B. Morton (UF: University of Florida)H-Index: 8
Last.Ashley J. Smuder (UF: University of Florida)H-Index: 29
view all 4 authors...
#1Toshinori Yoshihara (Juntendo University)H-Index: 10
#2Shuichi Machida (Juntendo University)H-Index: 10
Last.Hisashi Naito (Juntendo University)H-Index: 18
view all 6 authors...
#1Marco Alessandro Minetto (UNITO: University of Turin)H-Index: 23
#2Rizwan Qaisar (UNIPV: University of Pavia)H-Index: 10
Last.Roberto Bottinelli (UNIPV: University of Pavia)H-Index: 52
view all 8 authors...
#1Keita Kanzaki (Kurashiki Sakuyo University)H-Index: 5
#2Mai Kuratani (Hiroshima University)H-Index: 4
Last.Masanobu Wada (Hiroshima University)H-Index: 15
view all 5 authors...
Cited By2
#1Takashi Yamada (RMIT: RMIT University)H-Index: 23
#2Yuki Ashida (RMIT: RMIT University)H-Index: 2
Last.Koichi Himori (RMIT: RMIT University)H-Index: 4
view all 4 authors...
#1Maarten M. Steinz (KI: Karolinska Institutet)H-Index: 2
#2Malin Persson (McGill University)H-Index: 7
Last.Johanna T. Lanner (KI: Karolinska Institutet)H-Index: 18
view all 23 authors...