Effect of Functional Groups of Self-Assembled Monolayers on Protein Adsorption and Initial Cell Adhesion

Published on Sep 10, 2018in ACS Biomaterials Science & Engineering4.511
· DOI :10.1021/acsbiomaterials.8b00795
Abshar Hasan9
Estimated H-index: 9
(IITG: Indian Institute of Technology Guwahati),
Sudip K. Pattanayek11
Estimated H-index: 11
(IITD: Indian Institute of Technology Delhi),
Lalit M. Pandey13
Estimated H-index: 13
(IITG: Indian Institute of Technology Guwahati)
Surface modification plays a vital role in regulating protein adsorption and subsequently cell adhesion. In the present work, we prepared nanoscaled modified surfaces using silanization and characterized them using Fourier-transform infrared spectroscopy (FTIR), water contact angle (WCA), and atomic force microscopy (AFM). Five different (amine, octyl, mixed, hybrid, and COOH) surfaces were prepared based on their functionality and varying wettability and their effect on protein adsorption and initial cell adhesion was investigated. AFM analysis revealed nanoscale roughness on all modified surfaces. Fetal bovine serum (FBS) was used for protein adsorption experiment and effect of FBS was analyzed on initial cell adhesion kinetics (up to 6 h) under three different experimental conditions: (a) with FBS in media, (b) with preadsorbed FBS on surfaces, and (c) incomplete media, i.e., without FBS. Various cell features such as cell morphology/circularity, cell area and nuclei size were also studied for the abov...
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