Original paper
Quantitation of the monoclonal antibody Denosumab by bioassay and validated LC methods
Volume: 119, Pages: 96 - 104
Published: Nov 1, 2018
Abstract
The monoclonal antibody Denosumab (DmAb) is clinically used to treat osteoporosis and bone loss. We developed a bioassay based on the ability of DmAb to inhibit the effect of human receptor activator of nuclear factor-κB ligand (RANKL) to stimulate the formation of osteoclasts derived from RAW 264.7 cells. This bioassay was applied in conjunction with size exclusion high-performance liquid chromatography (SE–HPLC) and reversed-phase...
Paper Details
Title
Quantitation of the monoclonal antibody Denosumab by bioassay and validated LC methods
Published Date
Nov 1, 2018
Volume
119
Pages
96 - 104
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History