Identification and mechanism of effective components from rape (Brassica napus L.) bee pollen on serum uric acid level and xanthine oxidase activity

This study was aimed at evaluating the hypouricemic effect of rape bee pollen from Qinghai province in China. Rape bee pollen crude extracts (RPCE) was obtained by n-butanol extraction and purified by polyamide resin and AB-8 resin respectively to generate five fractions (fraction 1–5). In vitro, fraction 5 had strongest inhibition on xanthine oxidase (XO) (IC50 = 0.21 ± 0.02 mg mL−1) among five fractions and had reversible and mixed inhibitory...