Original paper
Tild-CRISPR Allows for Efficient and Precise Gene Knockin in Mouse and Human Cells
Abstract
The targeting efficiency of knockin sequences via homologous recombination (HR) is generally low. Here we describe a method we call Tild-CRISPR (targeted integration with linearized dsDNA-CRISPR), a targeting strategy in which a PCR-amplified or precisely enzyme-cut transgene donor with 800-bp homology arms is injected with Cas9 mRNA and single guide RNA into mouse zygotes. Compared with existing targeting strategies, this method achieved much...
Paper Details
Title
Tild-CRISPR Allows for Efficient and Precise Gene Knockin in Mouse and Human Cells
Published Date
May 1, 2018
Journal
Volume
45
Issue
4
Pages
526 - 536.e5
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Notes
History