Meiling Zhang
Shanghai Jiao Tong University
Publications 2
#1Meiling Zhang (SJTU: Shanghai Jiao Tong University)H-Index: 2
#2Changyang Zhou (CAS: Chinese Academy of Sciences)H-Index: 3
Last.Ning Yao (SJTU: Shanghai Jiao Tong University)H-Index: 2
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Base editing installs a precise nucleotide change in specific gene loci without causing a double-strand break. Its efficiency in human embryos is generally low, limiting its utility in functional genetic studies. Here, we report that injecting base editors into human cleaving two-cell and four-cell embryos results in much higher (up to 13-fold) homozygotic nucleotide substitution efficiency as opposed to MII oocytes or zygotes. Furthermore, as a proof-of-principle study, a point mutation can be ...
#1Yang Wang (SJTU: Shanghai Jiao Tong University)H-Index: 2
#2Meiling Zhang (SJTU: Shanghai Jiao Tong University)H-Index: 2
Last.Yanzhi Du (SJTU: Shanghai Jiao Tong University)H-Index: 2
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Vitrification of oocytes is closely associated with the lower embryonic developmental potential, which involves the cryopreservation injury occurred during vitrification. It indicates that vitrification may need to be further optimized. Therefore, we studied the effects of resveratrol, an antioxidant, on the developmental potential of vitrified mouse oocytes after in vitro fertilization. After adding a series of concentrations of resveratrol (0, 1, 10, 25, and 50 μM) into vitrification, warming,...
#1Xuan Yao (CAS: Chinese Academy of Sciences)H-Index: 7
#2Meiling Zhang (SJTU: Shanghai Jiao Tong University)H-Index: 2
Last.Yun Sun (SJTU: Shanghai Jiao Tong University)H-Index: 9
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Summary The targeting efficiency of knockin sequences via homologous recombination (HR) is generally low. Here we describe a method we call Tild-CRISPR (targeted integration with linearized dsDNA-CRISPR), a targeting strategy in which a PCR-amplified or precisely enzyme-cut transgene donor with 800-bp homology arms is injected with Cas9 mRNA and single guide RNA into mouse zygotes. Compared with existing targeting strategies, this method achieved much higher knockin efficiency in mouse embryos, ...
#1Changyang Zhou (CAS: Chinese Academy of Sciences)H-Index: 3
#2Meiling Zhang (SJTU: Shanghai Jiao Tong University)H-Index: 2
Last.Weiping Li (SJTU: Shanghai Jiao Tong University)H-Index: 7
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#1Cuiqing ZhongH-Index: 5
#2Meiling ZhangH-Index: 1
Last.Jinsong LiH-Index: 27
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Generation of human haploid embryonic stem cells from parthenogenetic embryos obtained by microsurgical removal of male pronucleus
#1Qiaoli Zhang (SJTU: Shanghai Jiao Tong University)H-Index: 1
#2Dong Liu (Tianjin Medical University)H-Index: 1
Last.Zi-Jiang Chen (SDU: Shandong University)H-Index: 36
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Brain-derived neurotrophic factor (BDNF) is expressed extensively in the mammalian female reproductive system and has been implicated in the development of follicles and oocytes. However, BDNF expression patterns in the ovary and its effects on oocyte maturation and embryonic development in polycystic ovary syndrome (PCOS) have not been established. In the present study, we established a PCOS model by treating the rats with insulin and human chorionic gonadotropin (hCG). Rats treated with insuli...