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Xuan Yao
Chinese Academy of Sciences
10Publications
8H-index
267Citations
Publications 10
Newest
#1Xinde Hu (CAS: Chinese Academy of Sciences)H-Index: 9
#2Jinghan Wang (Fudan University)
Last.Hui Yang (CAS: Chinese Academy of Sciences)H-Index: 19
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Source
#1Xuan Yao (CAS: Chinese Academy of Sciences)H-Index: 8
#2Meiling Zhang (SJTU: Shanghai Jiao Tong University)H-Index: 5
Last.Hui Yang (CAS: Chinese Academy of Sciences)H-Index: 19
view all 16 authors...
Summary The targeting efficiency of knockin sequences via homologous recombination (HR) is generally low. Here we describe a method we call Tild-CRISPR (targeted integration with linearized dsDNA-CRISPR), a targeting strategy in which a PCR-amplified or precisely enzyme-cut transgene donor with 800-bp homology arms is injected with Cas9 mRNA and single guide RNA into mouse zygotes. Compared with existing targeting strategies, this method achieved much higher knockin efficiency in mouse embryos, ...
19 CitationsSource
#1Xuan YaoH-Index: 8
#2Xing WangH-Index: 10
Last.Hui YangH-Index: 19
view all 6 authors...
As a promising genome editing platform, the CRISPR/Cas9 system has great potential for efficient genetic manipulation, especially for targeted integration of transgenes. However, due to the low efficiency of homologous recombination (HR) and various indel mutations of non-homologous end joining (NHEJ)-based strategies in non-dividing cells, in vivo genome editing remains a great challenge. Here, we describe a homology-mediated end joining (HMEJ)-based CRISPR/Cas9 system for efficient in vivo pre...
3 CitationsSource
#1Xuan Yao (CAS: Chinese Academy of Sciences)H-Index: 8
#2Zhen Liu (CAS: Chinese Academy of Sciences)H-Index: 12
Last.Hui Yang (CAS: Chinese Academy of Sciences)H-Index: 19
view all 10 authors...
14 CitationsSource
#1Haibo Zhou (CAS: Chinese Academy of Sciences)H-Index: 3
#2J.Q. Liu (CAS: Chinese Academy of Sciences)H-Index: 4
Last.Hui Yang (CAS: Chinese Academy of Sciences)H-Index: 19
view all 22 authors...
Despite rapid progresses in the genome-editing field, in vivo simultaneous overexpression of multiple genes remains challenging. We generated a transgenic mouse using an improved dCas9 system that enables simultaneous and precise in vivo transcriptional activation of multiple genes and long noncoding RNAs in the nervous system. As proof of concept, we were able to use targeted activation of endogenous neurogenic genes in these transgenic mice to directly and efficiently convert astrocytes into f...
44 CitationsSource
#1Erwei Zuo (CAS: Chinese Academy of Sciences)H-Index: 6
#2Xiaona Huo (CAS: Chinese Academy of Sciences)H-Index: 2
Last.Hui Yang (CAS: Chinese Academy of Sciences)H-Index: 19
view all 18 authors...
The CRISPR/Cas9 system has become an efficient gene editing method for generating cells carrying precise gene mutations, including the rearrangement and deletion of chromosomal segments. However, whether an entire chromosome could be eliminated by this technology is still unknown. Here we demonstrate the use of the CRISPR/Cas9 system to eliminate targeted chromosomes. Using either multiple cleavages induced by a single-guide RNA (sgRNA) that targets multiple chromosome-specific sites or a cockta...
34 CitationsSource
#1Erwei Zuo (CAS: Chinese Academy of Sciences)H-Index: 6
#2Yijun Cai (CAS: Chinese Academy of Sciences)H-Index: 8
Last.Hui Yang (CAS: Chinese Academy of Sciences)H-Index: 19
view all 29 authors...
One-step generation of complete gene knockout mice and monkeys by CRISPR/Cas9-mediated gene editing with multiple sgRNAs
47 CitationsSource
#1Xuan Yao (CAS: Chinese Academy of Sciences)H-Index: 8
#2Xing Wang (CAS: Chinese Academy of Sciences)H-Index: 10
Last.Hui Yang (CAS: Chinese Academy of Sciences)H-Index: 19
view all 11 authors...
Abstract Precisely targeted genome editing is highly desired for clinical applications. However, the widely used homology-directed repair (HDR)-based genome editing strategies remain inefficient for certain in vivo applications. We here demonstrate a microhomology-mediated end-joining (MMEJ)-based strategy for precisely targeted gene integration in transfected neurons and hepatocytes in vivo with efficiencies up to 20%, much higher (up to 10 fold) than HDR-based strategy in adult mouse tissues. ...
21 CitationsSource
#1Xuan Yao (CAS: Chinese Academy of Sciences)H-Index: 8
#2Xing Wang (CAS: Chinese Academy of Sciences)H-Index: 10
Last.Hui Yang (CAS: Chinese Academy of Sciences)H-Index: 19
view all 21 authors...
Abstract Targeted integration of transgenes can be achieved by strategies based on homologous recombination (HR), microhomology-mediated end joining (MMEJ) or non-homologous end joining (NHEJ). The more generally used HR is inefficient for achieving gene integration in animal embryos and tissues, because it occurs only during cell division, although MMEJ and NHEJ can elevate the efficiency in some systems. Here we devise a homology-mediated end joining (HMEJ)-based strategy, using CRISPR/Cas9-me...
58 CitationsSource
#1Lingling Miao (CAS: Chinese Academy of Sciences)H-Index: 9
#2Hailan Yao (CAS: Chinese Academy of Sciences)H-Index: 1
Last.Yizheng WangH-Index: 7
view all 9 authors...
MicroRNAs (miRNAs) can direct post-transcriptional or transcriptional gene silencing. Here, we report that miR-552 is in the nucleus and cytosol and inhibits human cytochrome P450 (CYP) 2E1 expression at both transcriptional and post-transcriptional levels. MiR-552 via its non-seed sequence forms hybrids with a loop hairpin of the cruciform structure in CYP2E1 promoter region to inhibit SMARCE1 and RNA polymerase II binding to the promoter and CYP2E1 transcription. Expressing SMARCE1 reverses th...
27 CitationsSource
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