IGFBP-3 plays an important role in senescence as an aging marker.

Published on Apr 1, 2018in Environmental Toxicology and Pharmacology3.061
· DOI :10.1016/j.etap.2018.03.014
Sugyeong Hong2
Estimated H-index: 2
(Dong-eui University),
Moon-Moo Kim20
Estimated H-index: 20
(Dong-eui University)
Abstract Aging study requires aging markers to measure the degree of aging process. The aging markers such as senescence associated-β-galactosidase (SA-β-gal), lipofuscin, telomere, p53 and p16 have been known in aging study until now. Therefore, we investigated the role of genes and proteins related to aging in young, senescent and H 2 O 2 -induced old cells to develop a novel aging marker involved in aging mechanism. After cellular aging was compared in young, senescent and H 2 O 2 -induced old cells using SA-β-galactosidase staining assay, the expression level of genes and proteins in senescent and H 2 O 2 -induced old cells were compared and analyzed with those of young cells using RT-PCR, western blot and immunofluorescence staining. First of all, the senescent cells and the cells aged by H 2 O 2 showed higher level of SA-β-galactosidase staining than young cells. In particular, the expression level of IGFBP-3 was decreased in senescent and H 2 O 2 -induced old cells compared with young cells. Moreover, the senescent and H2O2-induced old cells showed higher expression levels of p -PI3K, Akt-1, p -mTOR, p -FoxO1 and FoxO1 than young cells. Furthermore, the expression levels of p300, Ac-p53, p53, p -p21 and p16 were significantly increased in senescent and H 2 O 2 -induced cells compared to young cells. However, the expression level of SIRT-1 was decreased in senescent and H 2 O 2 -induced old cells compared to young cells. In conclusion, IGFBP-3 up-regulates PI3K/Akt/mTOR signaling pathway and down-regulates autophagy during cell aging. These results suggest that IGFBP-3 could play a key role in aging study as an important aging marker.
  • References (28)
  • Citations (2)
📖 Papers frequently viewed together
45 Citations
62 Citations
1 Citations
78% of Scinapse members use related papers. After signing in, all features are FREE.
6 CitationsSource
#1Karl-Heinz Wagner (University of Vienna)H-Index: 36
#2David Cameron-Smith (University of Vienna)H-Index: 48
Last. Bernhard Franzke (University of Vienna)H-Index: 9
view all 4 authors...
Aging is a major risk factor for most chronic diseases and functional impairments. Within a homogeneous age sample there is a considerable variation in the extent of disease and functional impairment risk, revealing a need for valid biomarkers to aid in characterizing the complex aging processes. The identification of biomarkers is further complicated by the diversity of biological living situations, lifestyle activities and medical treatments. Thus, there has been no identification of a single ...
44 CitationsSource
#1Pierpaola Davalli (University of Modena and Reggio Emilia)H-Index: 18
#2Tijana Mitic (Edin.: University of Edinburgh)H-Index: 9
Last. Domenico D'Arca (University of Modena and Reggio Emilia)H-Index: 13
view all 5 authors...
The aging process worsens the human body functions at multiple levels, thus causing its gradual decrease to resist stress, damage, and disease. Besides changes in gene expression and metabolic control, the aging rate has been associated with the production of high levels of Reactive Oxygen Species (ROS) and/or Reactive Nitrosative Species (RNS). Specific increases of ROS level have been demonstrated as potentially critical for induction and maintenance of cell senescence process. Causal connecti...
130 CitationsSource
#1Peter Bang (Linköping University)H-Index: 15
#2Anders Thorell (KI: Karolinska Institutet)H-Index: 47
Last. Jonas Nygren (KI: Karolinska Institutet)H-Index: 49
view all 6 authors...
Background: Patients receiving a carbohydrate drink (CHO) before major abdominal surgery display improved insulin sensitivity postoperatively and increased proteolysis of IGFBP-3 (IGFBP-3-PA) compa ...
1 CitationsSource
#1A. BernadotteH-Index: 3
Last. Spivak ImH-Index: 7
view all 3 authors...
The cellular senescence definition comes to the fact of cells irreversible proliferation disability. Besides the cell cycle arrest, senescent cells go through some morphological, biochemical, and functional changes which are the signs of cellular senescence. The senescent cells (including replicative senescence and stress-induced premature senescence) of all the tissues look alike. They are metabolically active and possess the set of characteristics in vitro and in vivo, which are known as bioma...
75 CitationsSource
#1David M. Dolivo (WPI: Worcester Polytechnic Institute)H-Index: 5
#2Sarah Hernandez (WPI: Worcester Polytechnic Institute)H-Index: 4
Last. Tanja Dominko (WPI: Worcester Polytechnic Institute)H-Index: 8
view all 3 authors...
The study of cellular senescence and proliferative lifespan is becoming increasingly important because of the promises of autologous cell therapy, the need for model systems for tissue disease and the implication of senescent cell phenotypes in organismal disease states such as sarcopenia, diabetes and various cancers, among others. Here, we explain the concepts of proliferative cellular lifespan and cellular senescence, and we present factors that have been shown to mediate cellular lifespan po...
11 CitationsSource
#1Bennett G. Childs (Mayo Clinic)H-Index: 8
#2Matej Durik (Mayo Clinic)H-Index: 3
Last. J. M. Van DeursenH-Index: 69
view all 4 authors...
Cellular senescence, a process that imposes permanent proliferative arrest on cells in response to various stressors, has emerged as a potentially important contributor to aging and age-related disease, and it is an attractive target for therapeutic exploitation. A wealth of information about senescence in cultured cells has been acquired over the past half century; however, senescence in living organisms is poorly understood, largely because of technical limitations relating to the identificati...
384 CitationsSource
view all 7 authors...
3 CitationsSource
#1Hamid Saeed (Panjab University, Chandigarh)H-Index: 7
#2Weimin Qiu (University of Southern Denmark)H-Index: 13
Last. Moustapha Kassem (KSU: King Saud University)H-Index: 69
view all 6 authors...
The contribution of deficient telomerase activity to age-related decline in osteoblast functions and bone formation is poorly studied. We have previously demonstrated that telomerase over-expression led to enhanced osteoblast differentiation of human bone marrow skeletal (stromal) stem cells (hMSC) in vitro and in vivo. Here, we investigated the signaling pathways underlying the regulatory functions of telomerase in osteoblastic cells. Comparative microarray analysis and Western blot analysis of...
9 CitationsSource
#1Nicole M. Ashpole (University of Oklahoma Health Sciences Center)H-Index: 17
#2Jessica E. Sanders (University of Oklahoma Health Sciences Center)H-Index: 1
Last. William E. SonntagH-Index: 60
view all 5 authors...
Abstract Growth hormone (GH) and insulin-like growth factor (IGF)-1 regulate the development and function of cells throughout the body. Several clinical diseases that result in a decline in physical and mental functions are marked by mutations that disrupt GH or IGF-1 signaling. During the lifespan there is a robust decrease in both GH and IGF-1. Because GH and IGF-1 are master regulators of cellular function, impaired GH and IGF-1 signaling in aging/disease states leads to significant alteratio...
54 CitationsSource
Cited By2
The insulin-like growth factor (IGF) family plays key roles in growth and development. In the cornea, IGF family members have been implicated in proliferation, differentiation, and migration, critical events that maintain a smooth refracting surface that is essential for vision. The IGF family is composed of multiple ligands, receptors, and ligand binding proteins. Expression of IGF type 1 receptor (IGF-1R), IGF type 2 receptor (IGF-2R), and insulin receptor (INSR) in the cornea has been well ch...
#1Won Kyong ChoH-Index: 19
Last. Jeong-Hun LeeH-Index: 2
view all 11 authors...
Edelweiss (Leontopodium Alpinum) in the family Asteraceae is a wildflower that grows in rocky limestone places. Here, we investigated the efficacy of edelweiss callus culture extract (Leontopodium Alpinum callus culture extract; LACCE) using multiple assays from in vitro to in vivo as well as transcriptome profiling. Several in vitro assay results showed the strong antioxidant activity of LACCE in response to UVB treatment. Moreover, LACCE suppressed inflammation and wrinkling; however, moisturi...
#1Hyeju Ko (Dong-eui University)
#2Moon-Moo Kim (Dong-eui University)H-Index: 20
The purpose of this study is to investigate the effect of H2O2 on the aging of melanogenesis in human melanocytes. The staining of SA-β-galactosidase, an aging marker, was remarkably increased in the cells aged with H2O2 at 62.5 µM or more compared with young cells. The intracellular H2O2 level and melanin synthesis were also reduced in both H2O2-treated cells and senescent cells compared with young cells in DCFH-DA assay. Both the senescent cells and the H2O2-treated cells showed higher express...
#1Jianguo Hu (CQMU: Chongqing Medical University)H-Index: 8
#2Li Liu (CQMU: Chongqing Medical University)
Last. Xiaojing Dong (CQMU: Chongqing Medical University)H-Index: 3
view all 12 authors...
LncRNA plays a crucial role in human disease. However, the expression and function of LncRNA in ICP(Intrahepatic cholestasis of pregnancy) is still not fully elucidated. In this study, we found Linc02527 was increased expression in placenta and serum of ICP patients. Ectopically expression of Linc02527 promoted autophagy and proliferate in HTR8 cells. Silencing Linc02527 suppressed the autophagy and proliferate in HTR8 cells. Mechanically study revealed that Linc02527 regulated the expression of...