Correlated Single Molecule Twist and Fluorescence Measurements on CRISPR/Cas Systems

CRISPR/Cas systems are RNA-guided ribonucleoprotein (RNP) complexes with nuclease activity that provide prokaryotes with an adaptive defense mechanism against foreign nucleic acids. The RNP complex recognizes complementary target sites by base-pairing its RNA component with one of the strands of the target DNA while displacing the other one forming a so-called R-loop structure. After completion of the R-loop formation, the target DNA is cleaved...