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Ralf Seidel
Leipzig University
138Publications
37H-index
3,840Citations
Publications 139
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#2M. RutkauskasH-Index: 9
Last.Ralf SeidelH-Index: 37
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#1Rosevalentine Bosire (University of Debrecen)
#2Péter P. Nánási (University of Debrecen)H-Index: 28
Last.Gábor Szabó (University of Debrecen)H-Index: 36
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The restricted access of regulatory factors to their binding sites on DNA wrapped around the nucleosomes is generally interpreted in terms of molecular shielding exerted by nucleosomal structure and internucleosomal interactions. Binding of proteins to DNA often includes intercalation of hydrophobic amino acids into the DNA. To assess the role of constrained superhelicity in limiting these interactions, we studied the binding of small molecule intercalators to chromatin in close to native condit...
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#1Ilaria Ceppi (USI: University of Lugano)
#2Sean Michael Howard (USI: University of Lugano)H-Index: 3
Last.Petr Cejka (USI: University of Lugano)H-Index: 28
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BLM or WRN helicases function with the DNA2 helicase-nuclease to resect DNA double-strand breaks and initiate homologous recombination. Upon DNA unwinding by BLM/WRN, RPA directs the DNA2 nuclease to degrade the 59-strand, revealing the 39 overhang needed for recombination. RPA bound to ssDNA also represents a barrier, explaining the need for the motor activity of DNA2 to displace RPA prior to resection. Using ensemble and single molecule biochemistry, we show that phosphorylated CtIP dramatical...
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#1Inga Songailiene (Vilnius University)H-Index: 2
#2M. Rutkauskas (Leipzig University)H-Index: 9
Last.Ralf Seidel (Leipzig University)H-Index: 37
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Summary The multi-subunit type I CRISPR-Cas surveillance complex Cascade uses its crRNA to recognize dsDNA targets. Recognition involves DNA unwinding and base-pairing between the crRNA spacer region and a complementary DNA strand, resulting in formation of an R-loop structure. The modular Cascade architecture allows assembly of complexes containing crRNAs with altered spacer lengths that promise increased target specificity in emerging biotechnological applications. Here we produce type I-E Cas...
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#1Richard Weichelt (TUD: Dresden University of Technology)H-Index: 2
#2Jingjing Ye (Leipzig University)H-Index: 2
Last.Ralf Seidel (Leipzig University)H-Index: 37
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#1Kristina Kasaciunaite (Leipzig University)H-Index: 2
#2Fergus Fettes (Leipzig University)H-Index: 1
Last.Ralf Seidel (WWU: University of Münster)H-Index: 37
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1 CitationsSource
#1Jingjing Ye (TUD: Dresden University of Technology)H-Index: 2
#2Seham Helmi (Leipzig University)H-Index: 2
Last.Ralf Seidel (TUD: Dresden University of Technology)H-Index: 37
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Recently introduced DNA nanomolds allow the shape-controlled growth of metallic nanoparticles. Here we demonstrate that this approach can be used to fabricate longer linear metal nanostructures of controlled lengths and patterns. To this end, we establish a set of different interfaces that enable mold interactions with high affinity and specificity. These interfaces enable and control the modular assembly of mold monomers into larger mold superstructure with programmable dimension in which each ...
2 CitationsSource
#1Jasmina Dikic (Leipzig University)H-Index: 1
#2Ralf Seidel (Leipzig University)H-Index: 37
Abstract DNA intercalators bind nucleic acids by stacking between adjacent basepairs. This causes a considerable elongation of the DNA backbone as well as untwisting of the double helix. In the past few years, single-molecule mechanical experiments have become a common tool to characterize these deformations and to quantify important parameters of the intercalation process. Parameter extraction typically relies on the neighbor-exclusion model, in which a bound intercalator prevents intercalation...
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#1Florian Scheffler (Leipzig University)
#2Mandy Brueckner (Leipzig University)H-Index: 1
Last.Uta Reibetanz (Leipzig University)H-Index: 11
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